Forest dynamics study using the methods in molecular ecological genetics

在本研究项目中，我们使用相对大面积的长期生态研究点和分子生态遗传学方法，研究了日本主要森林类型的森林群落组成的树木种群的结构和动态。具体目标是阐明种群在个体和DNA水平上的结构和动态以及生殖特征，包括生殖方式、交配系统、种子和花粉传播以及亲本的生殖成功，并解释和预测种群结构和动态，1.从个体水平上描述了思茅锥的种群结构和动态在暖温带万年青阔叶林中，温带针叶林中的钝齿Chamberparis obtuse、日本杉C.pisifera、崖柏Thujopsis dolabrata、皱叶栎Quercus crispula、倒卵叶木兰Magnolia obovata和白桦Betula grossa，以及温带针叶林中的红道云杉Picea jezoensis var.hondoensis、冷杉Abies mariesii、铁杉Tsuga diversifolia、 关于我们 2.利用微卫星标记技术，对天山冷杉、毛茛、白枝冬青和星叶冷杉进行了分子标记研究。3.描述了山茶、钝齿山茶和星叶山茶的遗传结构，这些遗传结构可能是由于有限的种子传播造成的。不同发育阶段的遗传结构程度不同。通过亲子关系分析，揭示了钝齿铁杉幼苗的半同胞结构。无性系生长对种群的维持和更新具有重要意义。5.对柳栎花粉流的亲子关系分析揭示了柳栎花粉流的模式。母树接受的花粉池存在遗传差异。6.建立了从个体水平和DNA水平解释和预测种群结构和动态以及繁殖过程的模型。对模型的适当性进行了评价。少

In this research project, we studied the structure and dynamics of tree populations composing forest communities of major forest types in Japan using long-term ecological research sites with relatively large areas and the methods in molecular ecological genetics. The specific objectives were to elucidate population structure and dynamics at individual and DNA levels and reproductive characteristics including modes of reproduction, mating system, seeds and pollen dispersals, and reproductive success of parents, and to explain and predict the population structure and dynamics, and reproductive process by modeling.1.Population structure and dynamics at the individual level were described for Castanopsis cuspidata var.sieboldii in a warm-temperate evergreen broad-leaved forest, Chamecyparis obtuse, C.pisifera, Thujopsis dolabrata, Quercus crispula, Magnolia obovata, and Betula grossa in a temperate coniferous forest, and Picea jezoensis var.hondoensis, Abies mariesii, Tsuga diversifolia, a … More nd Abies veitchii in a subalpine evergreen coniferous forest.2.Many microsatellite markers were developed for C.cuspidata var.sieboldii, Fagus crenata, Ilex leucoclada, and M.stellata. The markers were expected to be useful in molecular ecological genetic studies.3.Genetic structure within populations probably due to limited seed dispersal was described for Camellia japonica, F.crenata, and M.stellat a. The degrees of genetic structure were different between different stages. Half-sib structure of F.crenata saplings was revealed by parentage analysis.4.Clonal structure within populations was also described for I.leucoclada and C.pisifera. Clonal growth is found to be significant in maintaining and regenerating the populations.5.In Quercus salicina, the patterns of pollen flow were revealed by paternity analysis. The pollen pools accepted by mother trees were genetically different.6.Models were constructed to explain and predict the population structure and dynamics at the individual and DNA levels, and the reproductive process. The appropriateness of models was evaluated. Less