DNA repair defect, tumorigensis, and hereditary breast cancer gene Brca2 -Studies in vitro and in vivo, and association with Rad51.

DNA 修复缺陷、肿瘤发生和遗传性乳腺癌基因 Brca2 - 体外和体内研究以及与 Rad51 的关联。

• 批准号: 11460133
• 负责人: MORIMATSU Masami
• 金额: $ 8.32万
• 依托单位: Iwate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11460133/
• 关键词: breast cancer; dog; cat; mouse; Brca2; Rad51; IkB; 標的遺伝子組換え; 遺伝子機能解析

To establish the bases of developing new methods for prevention, diagnosis, and therapy of breast cancer , we investigated in vitro and invivo functions of the Brca2 and other tumor associated genes. Especially, researches for the significance of the interaction of Brca2 and Rad51, application of Brca2 analysis in veterinary medicine, and roles IkB MAIL were carried out.1. Cloning and mutation analysis of canine Brca2Full length (about 10 kbp) of the cDNA for canine Brca2 was cloned and sequenced. For mutation analysis, DNA fragments were obtained by PCR and used for protein truncation tests.2. Cloning and expression of feline Brca2Full length (about 10 kbp) of the cDNA for feline Brca2 was cloned and sequenced. Expression of feline Brca2 was confirmed in mamnary gland.3. Interaction of Brca2 and Rad51Interaction of C-terminus of canine Brca2 with Rad51 was demonstrated by yeast two-hybrid assay. Transactivation activity of canine Brca2 was also demonstrated.4. Characterization of a novel IkB protein, MAILWe cloned, sequenced, and analyzed the expression of the MAIL gene encoding a novel IkB protein.

为了建立开发用于预防，诊断和治疗乳腺癌的新方法的基础，我们研究了BRCA2和其他肿瘤相关基因的体外和Invivo功能。特别是，对BRCA2和RAD51相互作用的重要性，BRCA2分析在兽医医学中的应用以及IKB邮件的角色进行了研究。1。犬Brca2的犬Brca2full长度（约10 kbp）的克隆和突变分析被克隆和测序。为了突变分析，通过PCR获得DNA片段，并用于蛋白质截断测试。2。猫BRCA2的猫BRCA2FULL长度（约10 kbp）的克隆和表达被克隆并测序。猫BRCA2的表达在mamnary腺体中得到了证实。3。通过酵母两杂交测定法证明了BRCA2和RAD51的C端与RAD51的C端的相互作用。还证明了犬BRCA2的反式激活活性4。新型IKB蛋白的表征，邮递，测序和分析了编码新型IKB蛋白的邮件基因的表达。

项目成果

Iwasaki, K.: "Isolation, characterization, and cDNA cloning of chicken turpentine-induced protein, a new member of the scavenger receptor cystein-rich (SRCR) family of proteins"J. Biol. Chem.. 276. 9400-9405 (2001)

Iwasaki, K.：“鸡松节油诱导蛋白的分离、表征和 cDNA 克隆，该蛋白是清道夫受体富含半胱氨酸 (SRCR) 蛋白家族的新成员”J.

Ochiai, K.: "Cloning and sequencing full length of canine Brca2 and Read51 cDNA."J. Vet. Med. Sci. 63. 1103-1108 (2001)

Ochiai, K.：“犬 Brca2 和 Read51 cDNA 全长的克隆和测序。”

Kitamura, H.: "MAIL, a novel nuclear IkB protein that potentiates LPS-induced IL-6 production"FEBS Lett.. 485. 53-56 (2000)

Kitamura, H.：“MAIL，一种新型核 IkB 蛋白，可增强 LPS 诱导的 IL-6 产生”FEBS Lett.. 485. 53-56 (2000)

Nawa,A., et al.: "Tumor metastasis-associated human MTA1 gene : its deduced protein sequence, localization, and association with breast cancer cell proliferation using antisense phosphorothioate oligonucleotides"J Cell Biochem.. 79. 202-212 (2000)

Nawa,A., et al.：“肿瘤转移相关的人 MTA1 基因：使用反义硫代磷酸寡核苷酸推导的蛋白质序列、定位以及与乳腺癌细胞增殖的关联”J Cell Biochem.. 79. 202-212 (2000)

Watamabe,Y.ら: "Botulinum C3 enzyme changes the lactate dehydrogenase isozyme patten of primary culture of neurons"Journal of Veterinary Medical Science. (印刷中). (2000)

Watamabe, Y. 等人：“肉毒杆菌 C3 酶改变了神经元原代培养物的乳酸脱氢酶同工酶模式”《兽医医学科学》杂志（2000 年出版）。