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Analysis of Functional Regions on The Sendai Virus Genome

Analysis of Functional Regions on The Sendai Virus Genome
仙台病毒基因组功能区分析
批准号:
11470077
负责人:
KATO Atsushi
金额:
$4.42万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
1999
资助国家:
日本
项目状态:
已结题
起止时间:
1999 至 2001

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中文摘要
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英文摘要
An ORF overlapping the ammo-terminal portion of the Sendai virus (SeV) P ORF in the +1 frame produces a nested set of carboxy-coterminal proteins, C,' C, Yl and Y2, which are referred to collectively as the C proteins. The C proteins are an extremely versatile triple-role player ; they counteract the anti-viral action of interferons (IFNs), inhibit viral RNA synthesis and are involved in virus assembly. Here, we established HeLa cell lines stably expressing the C, Y1 and Y2 proteins individually and examined the capacity of these cells to circumvent the anti-viral action of IFNs and to inhibit the viral transcription. The data obtained clearly indicated that the smallest Y2 protein was as active as the C and Y1 proteins in both counteracting the anti-viral action of IFNs and inhibiting viral transcription.
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A. Kato, Y. Ohnishi, M. Kohase, S. Saito, M. Tashiro and Y. Nagai: "Y2, smallest of the Sendai virus C protein, is fully capable of both counteracting the antiviral action of interferons and inhibiting viral RNA synthesis"J. Virol. 75. 3802-3810 (2001)
A. Kato、Y. Ohnishi、M. Kohase、S. Saito、M. Tashiro 和 Y. Nagai:“Y2 是仙台病毒 C 蛋白中最小的一种,完全能够抵消干扰素的抗病毒作用并抑制病毒 RNA
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A.Kato他: "Sendai virus gene start signals are not equivalent in reinitiation capacity : Moderation at the fusion protein gene"Journal of Virology. 73. 9237-9246 (1999)
A. Kato 等人:“仙台病毒基因起始信号的重新启动能力不同:融合蛋白基因的调节”Journal of Virology 73. 9237-9246 (1999)。
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H.A.Koyama 他: "Lack of apoptosis in Sendai virus-infected HEp-2 cells without participation of viral antiapoptosis gene"Microbes Infect. 3. 1115-1121 (2001)
H.A.Koyama 等人:“在没有病毒抗凋亡基因参与的情况下,仙台病毒感染的 HEp-2 细胞缺乏细胞凋亡”Microbes Infect. 3. 1115-1121 (2001)。
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