Role of renal arginase in acute renal failure

肾精氨酸酶在急性肾衰竭中的作用

• 批准号: 11470219
• 负责人: TOMITA Kimio
• 金额: $ 9.34万
• 依托单位: Kumamoto University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11470219/
• 关键词: Nitric Oxide; arginase; acute renal failure; renal tubule; 虚血性急性腎不全; 尿細管; ミトコンドリア

Nitric Oxide has dual effects on cell physiology. Low concentration of Nitric Oxide has vasodilating effects on vascular system. High concentration of Nitric Oxide has toxic effects on cell. Low concentration of Nitric Oxide is useful for protecting renal function in acute renal failure. High concentration of Nitric Oxide should not be produced in renal tissue. We have investigated the arginase which convert arginine to substrate of NO ; urea and ornithine. We raised antibody to arginase II and investigated the localization of arginase II in the kidney. Distal tubule was strongly stained by anti-arginase II antibody. Cytosol was granularly stained in the cell, which suggests anginase II may be mitochondrial enzyme. This suggests that srginase II was mainly observed where are most fragile sites in the events of acute renal failure. So far, significant changes were not clearly observed, possibly because of the small changes of snginase II.It will be necessary to identify the locations by more sensitive methods such as laser capure microdissection. For the next projects, these methods should be investigated.

一氧化氮对细胞生理具有双重影响。一氧化氮对血管系统具有血管扩张作用。一氧化氮的高浓度对细胞有毒性作用。低浓度的一氧化氮可用于保护急性肾衰竭中的肾功能。高浓度的一氧化氮不应在肾组织中产生。我们已经研究了精氨酸酶，该精氨酸将精氨酸转化为NO的底物。尿素和鸟氨酸。我们提出了精氨酸酶II的抗体，并研究了精氨酸酶II在肾脏中的定位。远端小管被抗精氨酸酶II抗体强烈染色。细胞质在细胞中颗粒状染色，这表明烷吉酶II可能是线粒体酶。这表明Srginase II主要观察到急性肾衰竭事件中最脆弱的位点。到目前为止，还没有明确观察到重大变化，这可能是因为SNGINAPE II的较小变化。对于通过更敏感的方法（例如激光胶囊显微解剖）识别位置是必要的。对于下一个项目，应研究这些方法。

项目成果

Miyoshi T., G.Ooki, M.Murata, H.Hayakawa, K.Tomita, M.Imai, M.Suzuki.: "Expression of an mNSC1 in mammalian cells."Biochem.Biophys.Res.Commun.. 265. 13-17 (1999)

Miyoshi T.、G.Ooki、M.Murata、H.Hayakawa、K.Tomita、M.Imai、M.Suzuki.：“哺乳动物细胞中 mNSC1 的表达”。Biochem.Biophys.Res.Commun. 265。

Adachi M., K.Kitamura, T.Miyoshi T.Narikiyo, K.Iwashita, N.Shiraishi, H.Nonoguchi, K.Tomita.: "Activation of epithelial sodium channels by prostasin in Xenopus Oocytes."J.Am.Soc.Nephrol.. (in press).

Adachi M.、K.Kitamura、T.Miyoshi T.Narikiyo、K.Iwashita、N.Shiraishi、H.Nonoguchi、K.Tomita.：“爪蟾卵母细胞中前列腺素激活上皮钠通道。”J.Am.Soc

Miyanaka K., T.Gotoh, A.Nagasaki, M.Takeya, M.Ozaki, K.Iwase, M.Takiguchi, K.Tomita, M.Mori.: "Immunohistological localization of arginase II and other enzymes of arginine metabiolism in rat kidney and liver."Histochem.J.. 30. 741-751 (1998)

Miyanaka K.、T.Gotoh、A.Nagasaki、M.Takeya、M.Ozaki、K.Iwase、M.Takiguchi、K.Tomita、M.Mori.：“精氨酸酶 II 和其他精氨酸代谢酶的免疫组织学定位

Miyanaka K. et al: "Immunohistological localization of arginase II and other enzymes of arginine metabolism in rat kidney and liver."Histochem.J.. 30. 741-751 (1998)

Miyanaka K. 等人：“大鼠肾脏和肝脏中精氨酸酶 II 和其他精氨酸代谢酶的免疫组织学定位。”Histochem.J.. 30. 741-751 (1998)

Tashima, Y., Kohda, Y., Nonoguchi, H., Ikebe, M., Star, R.A., Tomita, K.: "Intranephron localization and regulation of V1a vasopressin receptor in chronic metabolic acidosis and dehydration in rats."Pflugers Arch.. (in press).

Tashima, Y.、Kohda, Y.、Nonoguchi, H.、Ikebe, M.、Star, R.A.、Tomita, K.：“大鼠慢性代谢性酸中毒和脱水中 V1a 加压素受体的肾内定位和调节。”Pflugers Arch