Antibody treatment against hypertension

抗高血压抗体治疗

• 批准号: 16390246
• 负责人: TOMITA Kimio
• 金额: $ 8.77万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390246/
• 关键词: Na channel; hypertension; serine protease; antibody; kidney

Sodium regulation in the kidney is one of the most important factors for the regulation of blood pressure. Epithelial sodium channel is key protein in the regulation of Na balance. We reported that a new serine protease prostasin can activate epithelial sodium channel in cultured cells. Prostasin was stimulated by aldosterone in vitro and in vivo experiments. In human, urinary prostasin excretion was elevated in primary aldosteronism, suggesting that prostasin may have significant roles in the regulation of blood pressure. Therefore, we have investigated to elucidate the roles of prostasin in the regulation of blood pressure by use of antibody against prostasin. At first, we have isolated several antibody against prostasin. We have already isolated one clones which inhibited prostasin activity. In addition, this antibody showed significant relation between urinary aldosterone excretion and urinary prostasin excretion in human samples.This projects is now underway. We must continue further experiments.

肾脏的钠调节是血压调节最重要的因素之一。上皮钠通道是调节钠平衡的关键蛋白。我们报道了一种新的丝氨酸蛋白酶前列腺素能激活培养细胞上皮钠通道。在体外和体内实验中，用醛固酮刺激前列腺素。在人类中，原发性醛固酮增多症患者尿前列腺素排泄量增加，提示前列腺素可能在血压调节中发挥重要作用。因此，我们利用前列腺素抗体来阐明前列腺素在血压调节中的作用。首先，我们分离了几种抗前列腺素的抗体。我们已经分离出一个抑制前列腺素活性的克隆。此外，这种抗体在人体样本中显示出尿醛固酮排泄和尿前列腺素排泄之间的显著关系。这项计划目前正在进行中。我们必须继续进行进一步的试验。

项目成果

Modulation of renal apical organic anion transporter 4 function by two PDZ domain-containing proteins

• DOI: 10.1681/asn.2005030306
• 发表时间: 2005-12-01
• 期刊: JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
• 影响因子: 13.6
• 作者: Miyazaki, H;Anzai, N;Endou, H
• 通讯作者: Endou, H

Regulation of aquaporin-2 by metabolic acidosis

代谢性酸中毒对 aquaporin-2 的调节

• 发表时间: 2004
• 期刊: Am J Physiol Cell Physiol 287
• 作者: Ohkouchi S;Nonoguchi H
• 通讯作者: Nonoguchi H

The multivalent PDZ domain-containing protein PDZK 1 regulates transport activity of renal urate-anion exchanger URAT1 via its C-terminal.

含有多价 PDZ 结构域的蛋白 PDZK 1 通过其 C 末端调节肾尿酸盐阴离子交换剂 URAT1 的转运活性。

• 发表时间: 2004
• 期刊: J Biol Chem 279
• 作者: Miyazaki H;他;Anzai N他
• 通讯作者: Anzai N他

Liddle's syndrome caused by a novel mutation in the proline-rich PYmotif of the epithelial sodium channel beta-subunit.

利德尔综合征由上皮钠通道β亚基富含脯氨酸的PY基序的新突变引起。

• 发表时间: 2005
• 期刊: J Clin Endocrinol Metab. 90
• 作者: Furuhashi M;他
• 通讯作者: 他

Differential regulation of Rad18 through Radio-dependent mono-and polyubiquitination.

通过无线电依赖性单泛素化和多泛素化对 Rad18 进行差异调节。

• 发表时间: 2005
• 期刊: J Biol Chem 28
• 作者: Miyase S;et al.
• 通讯作者: et al.