Microcirculatory effects of hyperglycemia on retinal ischemia

高血糖对视网膜缺血微循环的影响

• 批准号: 11470331
• 负责人: FUJITA Yoshihisa
• 金额: $ 3.65万
• 依托单位: KAWASAKI MEDICAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11470331/
• 关键词: microcirculation; retina; fluorescein microscope; ischemia; reperfusion; anesthetics; halothane; propofol; 局所麻酔薬; 高血糖; 測定法

Because propofol based anesthesia has several advantages over inhalational anesthetics for ophthalmic surgery, we decided to investigate the effects of propofol and halothane anesthesia on retinal circulation and function in a rabbit model of ocular ischemia. Methods : Incomplete retinal ischemia was induced in 17 pigmented Dutch rabbits with bilaterally ligated carotid arteries by deep anesthesia with propofol (propofol group, n=8) or by halothane (halothane group, n=9) to a mean arterial pressure of 40 mmHg. We evaluated retinal circulation by means of the dye dilution technique with a scanning laser ophthalmoscope and retinal function by examining the electroretinogram b-wave amplitude at the end of 60 min baseline, deep and recovery anesthesia. Results : Similar changes in ERG b-wave amplitudes were noted in both groups. The amplitudes in the propofol and halothane group decreased respectively to 63 % and 54 % of their baseline values during deep anesthesia, and fully recovered dur … More ing recovery anesthesia in both groups. Intraocular pressure in the propofol group decreased during deep anesthesia and remained less than the baseline value, while in the halothane group it remained unchanged. Segment retinal blood flow decreased in both groups during deep anesthesia. It increased during recovery anesthesia in the propofol group, but remained decreased in the halothane group. Conclusion : Evidence of the distinctive protective effects of propofol against ischemic injury to the retina could not be found in this study, when compared with halothane. Propofol anesthesia is characterized by a sustained lower IOP and prompt recovery of retinal blood flow. These may be suitable features for an anesthetic used for ophthalmic surgery.Methods : Incomplete retinal ischemia was induced in 17 pigmented Dutch rabbits with bilaterally ligated carotid arteries by deep anesthesia with propofol (propofol group, n=8) or by halothane (halothane group, n=9) to a mean arterial pressure of 40 mmHg. We evaluated retinal circulation by means of the dye dilution technique with a scanning laser ophthalmoscope and retinal function by examining the electroretinogram b-wave amplitude at the end of 60 min baseline, deep and recovery anesthesia. Results : Similar changes in ERG b-wave amplitudes were noted in both groups. The amplitudes in the propofol and halothane group decreased respectively to 63 % and 54 % of their baseline values during deep anesthesia, and fully recovered during recovery anesthesia in both groups. Intraocular pressure in the propofol group decreased during deep anesthesia and remained less than the baseline value, while in the halothane group it remained unchanged. Segment retinal blood flow decreased in both groups during deep anesthesia. It increased during recovery anesthesia in the propofol group, but remained decreased in the halothane group. Conclusion : Evidence of the distinctive protective effects of propofol against ischemic injury to the retina could not be found in this study, when compared with halothane. Propofol anesthesia is characterized by a sustained lower IOP and prompt recovery of retinal blood flow. These may be suitable features for an anesthetic used for ophthalmic surgery.Results : Similar changes in ERG b-wave amplitudes were noted in both groups. The amplitudes in the propofol and halothane group decreased respectively to 63 % and 54 % of their baseline values during deep anesthesia, and fully recovered during recovery anesthesia in both groups. Intraocular pressure in the propofol group decreased during deep anesthesia and remained less than the baseline value, while in the halothane group it remained unchanged. Segment retinal blood flow decreased in both groups during deep anesthesia. It increased during recovery anesthesia in the propofol group, but remained decreased in the halothane group. Conclusion : Evidence of the distinctive protective effects of propofol against ischemic injury to the retina could not be found in this study, when compared with halothane. Propofol anesthesia is characterized by a sustained lower IOP and prompt recovery of retinal blood flow. These may be suitable features for an anesthetic used for ophthalmic surgery.Conclusion : Evidence of the distinctive protective effects of propofol against ischemic injury to the retina could not be found in this study, when compared with halothane. Propofol anesthesia is characterized by a sustained lower IOP and prompt recovery of retinal blood flow. These may be suitable features for an anesthetic used for ophthalmic surgery. Less

由于异丙酚为基础的麻醉有几个优点，吸入麻醉剂的眼科手术，我们决定研究异丙酚和氟烷麻醉对视网膜循环和功能的影响，在兔眼缺血模型。研究方法：17只有色荷兰兔，双侧颈动脉结扎，用异丙酚（异丙酚组，n=8）或氟烷（氟烷组，n=9）麻醉至平均动脉压40 mmHg，诱导不完全性视网膜缺血。我们通过染料稀释技术和扫描激光检眼镜评估视网膜循环，通过检查60分钟基线、深度和恢复麻醉结束时的视网膜电图b波振幅评估视网膜功能。结果：两组的ERG b波振幅变化相似。异丙酚组和氟烷组在深麻醉时波幅分别下降至基线值的63%和54%， ...更多信息 两组均行恢复麻醉。异丙酚组的眼内压在深度麻醉期间下降，并保持低于基线值，而氟烷组保持不变。在深度麻醉期间，两组的节段视网膜血流均减少。异丙酚组在麻醉恢复期增加，氟烷组保持下降趋势。结论：与氟烷相比，在本研究中未发现丙泊酚对视网膜缺血性损伤具有独特保护作用的证据。异丙酚麻醉的特点是持续较低的IOP和迅速恢复视网膜血流。这些可能是合适的功能用于麻醉用于眼科surgery.Methods：不完全视网膜缺血诱导在17只有色荷兰兔双侧结扎颈动脉深麻醉异丙酚（异丙酚组，n=8）或氟烷（氟烷组，n=9）的平均动脉压为40毫米汞柱。我们通过染料稀释技术和扫描激光检眼镜评估视网膜循环，通过检查60分钟基线、深度和恢复麻醉结束时的视网膜电图b波振幅评估视网膜功能。结果：两组的ERG b波振幅变化相似。异丙酚组和氟烷组在深度麻醉时波幅分别下降至基线值的63%和54%，两组在恢复麻醉时均完全恢复。异丙酚组的眼内压在深度麻醉期间下降，并保持低于基线值，而氟烷组保持不变。在深度麻醉期间，两组的节段视网膜血流均减少。异丙酚组在麻醉恢复期增加，氟烷组保持下降趋势。结论：与氟烷相比，在本研究中未发现丙泊酚对视网膜缺血性损伤具有独特保护作用的证据。异丙酚麻醉的特点是持续较低的IOP和迅速恢复视网膜血流。这些可能是合适的功能用于麻醉眼科surgery.Results：在两组中注意到类似的变化，在ERG b波振幅。异丙酚组和氟烷组在深度麻醉时波幅分别下降至基线值的63%和54%，两组在恢复麻醉时均完全恢复。异丙酚组的眼内压在深度麻醉期间下降，并保持低于基线值，而氟烷组保持不变。在深度麻醉期间，两组的节段视网膜血流均减少。异丙酚组在麻醉恢复期增加，氟烷组保持下降趋势。结论：与氟烷相比，在本研究中未发现丙泊酚对视网膜缺血性损伤具有独特保护作用的证据。异丙酚麻醉的特点是持续较低的IOP和迅速恢复视网膜血流。这些可能是合适的功能用于麻醉用于眼科surgery.Conclusion：异丙酚对视网膜缺血性损伤的独特的保护作用的证据不能被发现在这项研究中，与氟烷相比。异丙酚麻醉的特点是持续较低的IOP和迅速恢复视网膜血流。这些可能是用于眼科手术的麻醉剂的合适特征。少