Effects of lidocaine on reperfusion injury of microcirculation -a study using the retinal microvessels-

本研究旨在探讨利多卡因对视网膜微循环中白细胞-内皮细胞相互作用的影响。由于通过由荧光活体显微镜和图像分析仪（Argus 20，Hamamatsu Photonics）组成的我们的系统不可能可视化视网膜微血管和在这些微血管中流动的血细胞，我们在仓鼠中进行了研究，其中显微镜观察室被植入背部皮肤褶皱上，并且在Sprague-Dawley大鼠中使用阴囊横纹肌进行了研究。实验动物分为利多卡因组（n=7）和对照组（n=7），利多卡因组以0.3mg/kg/hr的速度输注，对照组以等量生理盐水输注。与对照组相比，利多卡因组动物（血浆利多卡因浓度：6.1 *3.3 μ g/mL）中白细胞粘附到后小静脉内皮的能力减弱。毛细血管后微静脉上滚动的白细胞数量在对照组之间没有差异。 关于我们 OL和利多卡因组。在另一项研究中，我们试图用扫描激光检眼镜（SLO，Rodenstock，德国）观察兔视网膜微循环。结扎双侧颈总动脉后，用异丙酚或氟烷麻醉。控制性低血压的影响（平均动脉压=约。50 mmHg）对视网膜微循环的影响。两组均未观察到毛细血管渗漏或阻塞，提示异丙酚麻醉在眼科手术中的安全性。尽管该系统允许使用荧光素钠对视网膜微血管及其血浆流量进行可视化和定量分析，但它不能使用罗丹明6 G（一种用于微循环研究中白细胞染色的荧光染料）对视网膜微循环中的白细胞进行可视化。视网膜微循环中白细胞血流动力学的研究需要激光技术或染色技术的进步。少

The purpose of the research project was to examine the effects of lidocaine on leukocyte-endothelium interaction in retinal microcirculation. Because visualization of the retinal microvessels and blood cells flowing in these microvessels was not possible by our system consisting of a fluorescence vital microscope and an image analyzer (Argus 20, Hamamatsu Photonics), we performed the studies in hamsters where the microscopic observation chamber was implanted on the dorsal skin fold and in Sprague-Dawley rats using the scrotal striated muscle. Animals were divided into the lidocaine group (n=7) receiving lidocaine infusion 0.3mg/kg/hr and the control group (n=7) receiving the ame volume of saline. Leukocyte adhesion to the post-venular endothelium was attenuated in the animals of the lidocaine group (plasma lidocaine concentration : 6.1 *3.3 mu g/mL) as compared with those of the control group. The number of rolling leukocytes on the post-capillary venules did not differ among the contr … More ol and lidocaine groups. We concluded that attenuation of the leukocyte adhesion to the post-capillary venule may be responsible for the anti-inflammatory property of the lidocaine.In another study, we tried to visualize the retinal microcirculation in rabbits by using Scanning Laser Ophthalmoscopy (SLO, Rodenstock, Germany). Rabbits with ligation of bilateral carotid artery were anesthetized either with propofol or halothane. The effects of induced hypotension (mean arterial pressure=ca. 50 mmHg) by these anesthetics on retinal microcirculation were compared. Capillary leakage or blockage was observed in neither group, suggesting the safety of propofol anesthesia in ophthalmologic surgery.Although the system permits visualization and quantitative analysis of retinal inicrovessels and their plasma flow by using fluorescein sodium, it could not visualize leukocytes in the retinal microcirculation with rhodamine 6G, a fluorescent dye, which is used for staining leukocytes in microcirculation research. Advances in Laser technology or staining techniques are necessary for investigation of leukocyte hemodynamics in the retinal microcirculation. Less