Effects of lidocaine on reperfusion injury of microcirculation -a study using the retinal microvessels-

本研究旨在探讨利多卡因对视网膜微循环中白细胞-内皮细胞相互作用的影响。由于我们的系统由荧光生命显微镜和图像分析仪(Argus 20，Hamamatsu Photonics)组成，无法显示视网膜微血管和在这些微血管中流动的血细胞，因此我们在仓鼠身上进行了研究，其中显微镜观察室被植入到背侧皮肤皱褶上，并在Spraogue-Dawley大鼠身上使用阴囊横纹肌进行了研究。动物分为利多卡因组(n=7)和对照组(n=7)，利多卡因组给予利多卡因0.3 mg/kg/h静脉滴注，对照组给予生理盐水。与对照组相比，利多卡因组(血浆利多卡因血药浓度：6.1×3.3mU·g/L)的白细胞与静脉后内皮细胞的黏附作用减弱。在对照…中，毛细血管后小静脉上滚动的白细胞数量没有差别。羟乙醇组和利多卡因组较多。在另一项研究中，我们试图使用扫描激光眼底镜(SLO，Rodenstock，德国)来显示兔的视网膜微循环。结扎双侧颈总动脉的兔用异丙酚或氟烷麻醉。比较了这些麻醉剂控制性降压(平均动脉压约50毫米汞柱)对视网膜微循环的影响。两组均未发现毛细血管渗漏或堵塞，提示异丙酚麻醉在眼科手术中是安全的。尽管该系统可以使用荧光素钠对视网膜微血管及其血浆流动进行可视化和定量分析，但它不能使用荧光染料罗丹明6G显示视网膜微循环中的白细胞，罗丹明6G用于微循环研究中的白细胞染色。激光技术或染色技术的进步对于研究视网膜微循环中的白细胞血流动力学是必要的。较少

The purpose of the research project was to examine the effects of lidocaine on leukocyte-endothelium interaction in retinal microcirculation. Because visualization of the retinal microvessels and blood cells flowing in these microvessels was not possible by our system consisting of a fluorescence vital microscope and an image analyzer (Argus 20, Hamamatsu Photonics), we performed the studies in hamsters where the microscopic observation chamber was implanted on the dorsal skin fold and in Sprague-Dawley rats using the scrotal striated muscle. Animals were divided into the lidocaine group (n=7) receiving lidocaine infusion 0.3mg/kg/hr and the control group (n=7) receiving the ame volume of saline. Leukocyte adhesion to the post-venular endothelium was attenuated in the animals of the lidocaine group (plasma lidocaine concentration : 6.1 *3.3 mu g/mL) as compared with those of the control group. The number of rolling leukocytes on the post-capillary venules did not differ among the contr … More ol and lidocaine groups. We concluded that attenuation of the leukocyte adhesion to the post-capillary venule may be responsible for the anti-inflammatory property of the lidocaine.In another study, we tried to visualize the retinal microcirculation in rabbits by using Scanning Laser Ophthalmoscopy (SLO, Rodenstock, Germany). Rabbits with ligation of bilateral carotid artery were anesthetized either with propofol or halothane. The effects of induced hypotension (mean arterial pressure=ca. 50 mmHg) by these anesthetics on retinal microcirculation were compared. Capillary leakage or blockage was observed in neither group, suggesting the safety of propofol anesthesia in ophthalmologic surgery.Although the system permits visualization and quantitative analysis of retinal inicrovessels and their plasma flow by using fluorescein sodium, it could not visualize leukocytes in the retinal microcirculation with rhodamine 6G, a fluorescent dye, which is used for staining leukocytes in microcirculation research. Advances in Laser technology or staining techniques are necessary for investigation of leukocyte hemodynamics in the retinal microcirculation. Less