Effects of lidocaine on reperfusion injury of microcirculation -a study using the retinal microvessels-

利多卡因对微循环再灌注损伤的影响-利用视网膜微血管的研究-

基本信息

批准号：
09671592
负责人：
FUJITA Yoshihisa
金额：
$ 1.98万
依托单位：
KAWASAKI MEDICAL SCHOOL
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671592/
关键词：
microcirculation lidocaine leukocytes endothelium adhesion molecules microvessels vital microscopy fluorescence

项目摘要

The purpose of the research project was to examine the effects of lidocaine on leukocyte-endothelium interaction in retinal microcirculation. Because visualization of the retinal microvessels and blood cells flowing in these microvessels was not possible by our system consisting of a fluorescence vital microscope and an image analyzer (Argus 20, Hamamatsu Photonics), we performed the studies in hamsters where the microscopic observation chamber was implanted on the dorsal skin fold and in Sprague-Dawley rats using the scrotal striated muscle. Animals were divided into the lidocaine group (n=7) receiving lidocaine infusion 0.3mg/kg/hr and the control group (n=7) receiving the ame volume of saline. Leukocyte adhesion to the post-venular endothelium was attenuated in the animals of the lidocaine group (plasma lidocaine concentration : 6.1 *3.3 mu g/mL) as compared with those of the control group. The number of rolling leukocytes on the post-capillary venules did not differ among the contr … More ol and lidocaine groups. We concluded that attenuation of the leukocyte adhesion to the post-capillary venule may be responsible for the anti-inflammatory property of the lidocaine.In another study, we tried to visualize the retinal microcirculation in rabbits by using Scanning Laser Ophthalmoscopy (SLO, Rodenstock, Germany). Rabbits with ligation of bilateral carotid artery were anesthetized either with propofol or halothane. The effects of induced hypotension (mean arterial pressure=ca. 50 mmHg) by these anesthetics on retinal microcirculation were compared. Capillary leakage or blockage was observed in neither group, suggesting the safety of propofol anesthesia in ophthalmologic surgery.Although the system permits visualization and quantitative analysis of retinal inicrovessels and their plasma flow by using fluorescein sodium, it could not visualize leukocytes in the retinal microcirculation with rhodamine 6G, a fluorescent dye, which is used for staining leukocytes in microcirculation research. Advances in Laser technology or staining techniques are necessary for investigation of leukocyte hemodynamics in the retinal microcirculation. Less

研究项目的目的是检查利多卡因对视网膜微循环中白细胞 - 内皮相互作用的影响。 Because visualization of the retinal microvessels and blood cells flowing in these microvessels was not possible by our system consisting of a fluorescence vital microscope and an image analyzer (Argus 20, Hamamatsu Photonics), we performed the studies in hamsters where the microscopic observation chamber was implanted on the dorsal skin fold and in Sprague-Dawley rats using the scrotal strated muscle.将动物分为利多卡因组（n = 7），接受利多卡因输注0.3mg/kg/hr，对照组（n = 7）接受了AME盐水量。与对照组相比，在利多卡因组的动物（血浆利多卡因浓度：6.1 *3.3 mu g/ml）中，白细胞粘附于植物后植物的粘附于植物后植物。在con中，毛细血管后脉搏上滚动白细胞的数量没有差异……更多的OL和利多卡因组。我们得出的结论是，白细胞粘附对毛细血管后视网膜的粘附可能是利多卡因的抗炎特性的原因。在另一项研究中，我们试图通过使用扫描的视网膜微循环可视化兔子在兔子中使用扫描激光镜（Slo，Rodenstock，Rodenstock，dermany）。用提案或硫烷麻醉双侧颈动脉的兔子。比较了这些麻醉药对视网膜微循环的诱导低血压（平均动脉压=Ca。50mmHg）的影响。 Capillary leakage or blockage was observed in either group, suggesting the safety of proposal anesthesia in ophthalmologic surgery.Although the system permits visualization and quantitative analysis of retinal inicrovessels and their plasma flow by using fluorescein sodium, it could not visualize Leukocytes in the retinal microcirculation with rhodamine 6G, a fluorescent dye, which is used for staining微循环研究中的白细胞。激光技术或染色技术的进步对于在视网膜微循环中投资白细胞血液动力学是必需的。较少的