Factors involved in the interactions of Candida albicans and oral bacteria

通过监测生长介质中pH值的变化，研究了单个白色念珠菌在唾液、血清涂层或无蛋白(未涂层)、热循环的七种商业弹性衬里材料上的生长情况。用三个参数观察了组织调节剂对真菌生长的抑制作用：1)pH快速下降开始的延迟，2)pH变化速度的减慢，3)pH达到极小值。在对照软衬的情况下(不是热循环和未涂覆的)，抗真菌效果似乎取决于所使用的商业软衬的类型。因此，氟化硅和热固化硅材料的初始pH下降延迟，并观察到非常高的pH最小值。冷固化的丙烯酸软衬的最低pH值也很高，而冷固化的有机硅材料与热固化的丙烯酸树脂没有显著差异(p&gt；0.05)。然而，通过热循环(…)，材料的抗真菌效果显著降低更多的方差分析；p&lt；0.01)和一层蛋白质涂层(唾液，p&lt；0.05；血清，p&lt；0.01)。在第二项研究中，通过三磷酸腺苷分析，研究了一株白色念珠菌在唾液、血清涂层或无蛋白(未涂层)、热循环(分别为4oC-70oC，0,1000和10000倍)7种商业软衬材料上的生长情况。在对照弹性衬垫的情况下(不是热循环和未涂覆的)，真菌定植似乎取决于所使用的商业弹性衬垫的类型。因此，氟化和热固化有机硅材料的定植能力最低，冷固化有机硅材料除一种产品外，热固化的丙烯酸树脂的定植能力最高，冷固化的丙烯酸酯弹性衬里的定植能力居中。然而，热循环(ANOVA；p&lt；0.01)和一层蛋白质涂层(唾液，p&lt；0.01；血清，p&lt；0.01)显著促进了真菌在材料上的定植。综上所述，这些结果表明材料的老化和宿主的生物液促进了酵母在弹性衬里材料上的定植。较少

The growth of a single isolate of C.albicans on saliva-, serum-coated or protein free (uncoated), thermocycled seven commercial resilient lining materials was investigated, by monitoring pH changes in growth media. The inhibitory effect of the tissue conditioners on fungal growth was observed using three parameters viz : i) delay in the onset of the rapid decline in pH, ii) reduction in the rate of pH change and iii) the pH minima reached. In the case of control soft liners (not thermocycled and uncoated), the antifungal effect appeared to depend upon the type of commercial soft liner used. Thus, an initial delay in pH decline, and very high pH minima were observed with fluoric and heat cured silicone materials. High pH minima were also observed with cold cured acrylic soft liners, whereas cold cured silicone materials, did not significantly differ from heat cured acrylic resin (p>0.05). However, the antifungal effect of the materials was significantly reduced both by thermal cycling ( … More ANOVA ; p<0.01) and a layer of protein coating (saliva, p<0.05 ; serum, p<0.01). These results, taken together, suggest that the ageing of the materials and the biological fluids of the host, particularly serum, promote yeast growth on soft lining materials.In the second study, the growth of a single isolate of C.albicans on saliva-, serum-coated or protein free (uncoated), thermocycled (4oC-70oC for 1min, respectively ; 0, 1000 and 10000 times) seven commercial soft lining materials were investigated, by adenosine triphosphate (ATP) analysis. In the case of control resilient liners (not thermocycled and uncoated), the fungal colonization appeared to depend upon the type of commercial resilient liner used. Thus, the lowest colonization was observed with fluoric and heat cured silicone materials, cold cure silicone materials, except for one product, and heat cured acrylic resin exhibited the highest colonization capacity, and cold cured acrylic resilient liners exhibited the intermediate. However, the fungal colonization on the materials was significantly promoted both by thermal cycling (ANOVA ; p<0.01) and a layer of protein coating (saliva, p<0.01 ; serum, p<0.01). These results, taken together, suggest that the aging of the materials and the biological fluids of the host promote yeast colonization on resilient lining materials. Less