Factors involved in the interactions of Candida albicans and oral bacteria

白色念珠菌与口腔细菌相互作用的相关因素

• 批准号: 11470415
• 负责人: NIKAWA Hiroki
• 金额: $ 9.09万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11470415/
• 关键词: Candida albicans; Candida; AIDS; Histatin 5; dental material; soft lining meterial; inflammation; gingival epithelial cell; biofilm; serum

The growth of a single isolate of C.albicans on saliva-, serum-coated or protein free (uncoated), thermocycled seven commercial resilient lining materials was investigated, by monitoring pH changes in growth media. The inhibitory effect of the tissue conditioners on fungal growth was observed using three parameters viz : i) delay in the onset of the rapid decline in pH, ii) reduction in the rate of pH change and iii) the pH minima reached. In the case of control soft liners (not thermocycled and uncoated), the antifungal effect appeared to depend upon the type of commercial soft liner used. Thus, an initial delay in pH decline, and very high pH minima were observed with fluoric and heat cured silicone materials. High pH minima were also observed with cold cured acrylic soft liners, whereas cold cured silicone materials, did not significantly differ from heat cured acrylic resin (p>0.05). However, the antifungal effect of the materials was significantly reduced both by thermal cycling ( … More ANOVA ; p<0.01) and a layer of protein coating (saliva, p<0.05 ; serum, p<0.01). These results, taken together, suggest that the ageing of the materials and the biological fluids of the host, particularly serum, promote yeast growth on soft lining materials.In the second study, the growth of a single isolate of C.albicans on saliva-, serum-coated or protein free (uncoated), thermocycled (4oC-70oC for 1min, respectively ; 0, 1000 and 10000 times) seven commercial soft lining materials were investigated, by adenosine triphosphate (ATP) analysis. In the case of control resilient liners (not thermocycled and uncoated), the fungal colonization appeared to depend upon the type of commercial resilient liner used. Thus, the lowest colonization was observed with fluoric and heat cured silicone materials, cold cure silicone materials, except for one product, and heat cured acrylic resin exhibited the highest colonization capacity, and cold cured acrylic resilient liners exhibited the intermediate. However, the fungal colonization on the materials was significantly promoted both by thermal cycling (ANOVA ; p<0.01) and a layer of protein coating (saliva, p<0.01 ; serum, p<0.01). These results, taken together, suggest that the aging of the materials and the biological fluids of the host promote yeast colonization on resilient lining materials. Less

通过监测生长介质的pH变化，研究了单株白色念珠菌在唾液、血清包被或无蛋白（未包被）热循环的七种商业弹性衬里材料上的生长情况。通过三个参数(1)延缓pH快速下降的开始，2)降低pH变化的速率，3)pH达到最小值来观察组织调理剂对真菌生长的抑制作用。在对照软衬垫（非热循环和未涂层）的情况下，抗真菌效果似乎取决于所使用的商业软衬垫的类型。因此，在氟化和热固化有机硅材料中观察到pH值下降的初始延迟和非常高的pH值。冷固化丙烯酸软衬里也有较高的pH值，而冷固化硅树脂材料与热固化丙烯酸树脂没有显著差异（p>0.05）。然而，热循环（p<0.01）和蛋白包被（唾液，p<0.05；血清，p<0.01）均显著降低了材料的抑菌效果。综上所述，这些结果表明，材料的老化和宿主的生物液体，特别是血清，促进了酵母在软衬里材料上的生长。在第二项研究中，通过三磷酸腺苷（ATP）分析，研究了单株白色念珠菌在唾液、血清包被或无蛋白（未包被）、热循环（4℃-70℃分别为1min； 0次、1000次和10000次）七种商业软衬材料上的生长情况。在对照弹性衬垫（非热循环和未涂覆）的情况下，真菌的定植似乎取决于所使用的商业弹性衬垫的类型。因此，氟和热固化有机硅材料的定殖能力最低，冷固化有机硅材料除一种产品外，热固化丙烯酸树脂的定殖能力最高，而冷固化丙烯酸弹性衬垫则表现出中间产物。热循环（ANOVA, p<0.01）和蛋白包被（唾液，p<0.01；血清，p<0.01）均显著促进真菌在材料上的定植。综上所述，这些结果表明，材料的老化和寄主的生物流体促进了酵母在弹性衬里材料上的定植。少

项目成果

Makihira,S.,Nikawa,H. et al.: "Growth of Candida species on commercial denture adhesives in vitro."Int J Prosthodont. 14. 48-52 (2001)

牧平，S.，二川，H.

Nikawa,H. et al.: "Candida albicans colonization on thermal cycled maxillofacial polymeric materials in vitro"J Oral Rehabil. 27(in press). (2001)

二川，H.

Nikawa,H. et al.: "Effect of serum concentration on Candida biofilm formation on acrylic."Mycoses. 43. 139-143 (2000)

二川，H.

Nikawa, H.et al.: "Effect of serum concentration on Candida biofilm formation on acrylic."Mycoses. 43. 139-143 (2000)

Nikawa, H.等人：“血清浓度对丙烯酸树脂上念珠菌生物膜形成的影响。”真菌病。

Makihira, S., Nikawa, H.et al.: "Growth of Candida species on commercial denture adhesives in vitro."Int J Prosthodont. 14. 48-52 (2001)

Makihira, S., Nikawa, H.等人：“体外商业假牙粘合剂上念珠菌的生长。”Int J Prosthodont。