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Reduction of oral carriage of periodontal bacteria by the use of the induction of beta defensin through molecular pattern recognition into saliva

通过唾液中的分子模式识别诱导β防御素，减少牙周细菌的口腔携带

基本信息

批准号：
18390516
负责人：
NIKAWA Hiroki
金额：
$ 11.07万
依托单位：
Hiroshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18390516/
关键词：
saliva beta defensin periodontal bavteria citric acid βデフェンシンガムクエン酸

项目摘要

Objective: In a preliminary study, we noted that citric acid has the potency to induce human beta defensins (hBD-2) in salivary secretions. As hBDs are thought to ameliorate periodontal disease by suppressing key periodontal pathogens we developed a citric acid-containing chewing gum (CA-gum) to test this hypothesis. Here we report, the effect of CA-gum on three major periodontal pathogens Prevotella intermedia (Pi), Tannerella forsythia (Tf), Treponema denticola (Td) and Fusobacterium species (Fb).Methods: A randomized, double-blind, placebo-controlled trial with 61 individuals, was conducted over 14 days. Each subject was allowed to chew CA-gum (31 subjects) or placebo gum (30 subjects) at irregular intervals, but was instructed to eat at least three pieces of gum a day. None had active caries, symptoms of gingivitis or periodontal disease, smoking history, or concurrent medication. To determine the levels of oral carriage of the putative pathogens, saliva was collected from each subject by paraffin chewing in a standard manner and, the number of total cultivative microorganisms (CFUs) determined. Five key periodontal pathogens, Pg, Pi, TI Td and Fb present in saliva were determined quantitatively using real-time polymerase chain reaction (PCR) (BML Inc., Saitama, JAPAN).Results: Compared with the placebo chewing gum group the CA-gum group had significantly lower levels of Prevotella intermedia, Tannerella forsythia, and Fusobacterium spp. in their saliva. (Wilcoxon t-test; p<0.01). However, chewing of either type of gum did not affect the salivary carriage of total cultivative bacteria (Wilcoxon t-test; p>0.05). Conclusion: Consumption of citric acid-laced chewing gum reduces significantly the burden of periodontal pathogens in saliva.

目的：在一项初步研究中，我们注意到柠檬酸在唾液分泌物中具有诱导人β防御素（hBD-2）的效力。由于hBDs被认为通过抑制关键的牙周病原体来改善牙周病，我们开发了一种含柠檬酸的口香糖（CA-gum）来验证这一假设。本文报道了CA-gum对三种主要牙周病原体中间普氏菌（Pi）、连翘单宁菌（Tf）、密螺旋体（Td）和梭杆菌（Fb）的影响。方法：采用随机、双盲、安慰剂对照试验，对61例患者进行为期14天的研究。每位受试者被允许不定期咀嚼ca口香糖（31名受试者）或安慰剂口香糖（30名受试者），但被要求每天至少吃三片口香糖。没有活动性龋齿、牙龈炎或牙周病症状、吸烟史或同时用药。为了确定假定病原体的口腔携带水平，以标准方式用石蜡咀嚼法收集每个受试者的唾液，并测定总培养微生物（cfu）的数量。采用实时聚合酶链反应（real-time polymerase chain reaction， PCR）技术（BML Inc., Saitama， JAPAN）定量检测唾液中5种关键牙周病原体Pg、Pi、TI、Td和Fb。结果：与安慰剂口香糖组相比，ca口香糖组唾液中普雷沃氏菌、连翘单宁菌和梭杆菌的含量明显降低。（Wilcoxon t检验；p<0.01）。然而，咀嚼任何一种口香糖都不影响唾液中总培养细菌的携带（Wilcoxon t检验；p < 0.05）。结论：食用含柠檬酸的口香糖可显著减少唾液中牙周病原体的负担。