CHAR OF ESTROGEN BINDING PROTEIN FROM CANDIDA ALBICANS: AIDS OI

白念珠菌是最常见的机会性真菌 人体内的真菌病原体。最近的研究表明，当 将雌激素应用于白色念珠菌的培养，细胞经历 形态变化到更具传染性的有机体形式。 基于这一线索，来自白色念珠菌的一种雌激素结合蛋白 被斯坦福大学的一个团队分离和克隆。蛋白质显示 46%的序列与含有黄素的氧化还原蛋白同源性 对酿酒酵母的研究已有多年，但对于 其功能目前尚不清楚。我们已经开始合作了 表征催化剂的活性范围、氧化还原性质和 白念珠菌这种蛋白的配基结合行为 最终目标是确定其真正的功能和可能的关系 为传染性干杯。我们目前正在从我们的 斯坦福大学的合作者已经表达为一种融合蛋白 在大肠杆菌中表达，纯化后进行蛋白水解性剪裁。按顺序 将该重组蛋白与野生型进行性质比较 蛋白质，我们已经从一种结构性表达的 假丝酵母菌的菌株，并已表明它具有类似的活性和 分子量特性。在继续详细介绍之前 在定性上，我们想要确定的是，这部《野生型》 我们分离的蛋白质确实和重组的一样。在……里面 此外，同源基因至少有两个相似的基因 酵母中的蛋白质和我们的合作者已经表明 他们还克隆了念珠菌的第二个基因。因此，我们可能会有一个 纯化样品中同工酶的混杂。在这一点上我们会 想要确定我们是分离出了单一蛋白质还是 同工酶的混合物。那么另外呢，我们想要获得和比较 重组蛋白和“野生型”蛋白的序列数据验证 我们已经分离出正确的蛋白质。

Candida albicans is the most commonly occurring opportunistic fungal pathogen in humans. It has recently been shown that when estrogens are applied to cultures of C. albicans, the cells undergo a morphological change to the more infectious form of the organism. Based on this lead, an estrogen-binding protein from C. albicans has been isolated and cloned by a group at Stanford. The protein shows 46% sequence identity to a flavin containing redox protein from Saccharomyces cerevisiae that has been studied for some years, but for which the function is still not known. We have begun a collaboration to characterize the scope of catalytic activity, redox properties and ligand binding behavior of this protein from C. albicans, with the eventual goal of defining its true function and possible relationship to infectivity. We are currently receiving protein from our collaborators at Stanford that has been expressed as a fusion protein in E. coli and proteolytically clipped after purification. In order to compare the properties of this recombinant protein with wild type protein, we have purified enzyme from a constitutively expressing strain of Candida and have shown it to have similar activity and molecular weight properties. Before proceeding with detailed characterization, we would like to determine whether the "wild type" protein we have isolated is indeed the same as the recombinant. In addition, there are at least two similar genes for the homologous protein in Saccharomyces and our collaborators have indicated that they have also cloned a second gene in Candida. Thus, we may have a misture of isozymes in the purified sample. At this point we would like to determine whether we have isolated a single protein or a mixture of isozymes. Then in addition, we want to obtain and compare sequence data for both recombinant and "wild type" proteins to verify that we have isolated the correct protein.