Ion Trap mass spectrometer coupled to an HPLC including PC and nitrogen generator

离子阱质谱仪与包含 PC 和氮气发生器的 HPLC 联用

• 批准号: 456365157
• 金额: --
• 依托单位: Georg-August-Universität Göttingen
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2021
• 资助国家: 德国
• 起止时间: 2020-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/456365157?language=en
• 关键词: Ion; Trap; mass; spectrometer; coupled

The ion trap mass spectrometer will primarily be used for the de novo structural elucidation of primary and secondary plant compounds, the validation of known substances and the quantification of these plant compounds. A particular advantage of the ion trap mass spectrometer is the sequential fragmentation of the analyte into daughter ion(s). Multiple fragmentation (MSn) cannot be achieved with any other type of mass spectrometer. The ion trap mass spectrometer is coupled to a HPLC, which is suitable for high sample throughput.The ion trap mass spectrometer is used in various projects, e.g. (1) for de novo structure elucidation and quantification of complex flavonoid glycosides and hydroxycinnamic acid derivatives. Due to the lack of reference substances for complex compounds of this group of substances, a chromatographic analysis only is not possible. (2) to validate carotenoid stereoisomers for which no reference substances are available. In many cases, measurement with a mass spectrometer can provide additional information in this case, since chromatographic methods cannot provide sufficient differentiation. Their ionization is only possible with the help of the corona discharge needle of an APCI source without additives. (3) to validate the structural proposals of chlorophylls and their degradation products, which occur in very low concentrations. (4) to protect the proposed structure of glucosinolates which are precursors for health-protective isothiocyanates or other glucosinolate degradation products. (5) for de novo structure elucidation and validation of the proposed structure of sesquiterpene lactones, alkaloids and polyacetylenes, which are chemically diverse bitter substances in plants. (6) for the analysis of saponins in terms of qualification and quantification, since these substances cannot be detected by chromatography due to the lack of chromophores. (7) for the analysis of intact phospholipids, which has not yet been successful. Since the compounds are isotopically labelled, a high resolution of the isotope peaks is advantageous. (8) for the analysis of polysaccharides without derivatisation or total hydrolysis. Sequential fragmentation can produce oligosaccharides from the polysaccharides, which can be used for analysis and thus speed up the overall analysis. For these investigations, measurements up to MS11 are conceivable and will be tested.The ion trap mass spectrometer is installed in the HPLC-MS Service Unit of the Faculty of Agricultural Sciences.

离子阱质谱仪将主要用于一级和二级植物化合物的从头结构解析、已知物质的验证以及这些植物化合物的定量。离子阱质谱仪的一个特别优点是将分析物顺序碎片化为子离子。多重碎片（MSn）不能用任何其他类型的质谱仪实现。离子阱质谱仪与高效液相色谱仪耦合，可实现高样品通量。离子阱质谱仪可用于各种项目，例如：（1）复杂黄酮苷和羟基肉桂酸衍生物的从头结构解析和定量。由于缺乏这组物质的复杂化合物的参比物质，因此不可能仅进行色谱分析。(2)验证没有参考物质的类胡萝卜素立体异构体。在许多情况下，由于色谱方法无法提供足够的区分，因此使用质谱仪进行测量可以提供额外的信息。它们的电离只有在没有添加剂的APCI源的电晕放电针的帮助下才有可能。(3)以验证叶绿素及其降解产物的结构建议，这发生在非常低的浓度。(4)以保护硫代葡萄糖苷的结构，所述硫代葡萄糖苷是保护健康的异硫氰酸酯或其它硫代葡萄糖苷降解产物的前体。(5)用于倍半萜内酯、生物碱和聚乙炔的从头结构解析和拟议结构验证，这些物质是植物中化学上不同的苦味物质。(6)用于皂苷的定性和定量分析，因为这些物质由于缺乏发色团而不能通过色谱法检测。(7)用于完整磷脂的分析，这还没有成功。由于化合物是同位素标记的，因此同位素峰的高分辨率是有利的。(8)用于分析多糖而无需衍生或完全水解。连续片段化可以从多糖中产生寡糖，其可以用于分析，从而加速整体分析。对于这些调查，测量到MS 11是可以想象的，并将进行测试。离子阱质谱仪安装在农业科学学院的HPLC-MS服务单元。