Epidemiological investigation of protozoan diseases in China

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1)Cloning of genes encoding immunodominant antigens of protozoan parasitesIn the present study, many genes encoding irnmunodominat antigens of protozoan parasites were identified, cloned and characterized. The main genes are as follows : B.caballi P48, P134;B.equi EMA-1,EMA-2,P82;B.gibsoni P18,P29,P50;N.caninum SAG1,SRS2;T.gondii SAG1,SAG2,SRS1,ROP-1.2)Development of serodiagnostic methods for detection of antibodies to protozoan parasitesThe genes encoding immunodominant antigens were expressed in E.coli or insect cells, and then established the enzyme-linked immunosorbent assay, latex agglutination test, and immunochromatographic test with these recombinant antigens.3)Development of polymerase chain reactions for detection of protozoan parasitesThe primer sets based on genes described above were designed, and established PCR methods for detection of protozoan parasites.4)Epidemiological investigation of protozoan diseases in ChinaBlood samples collected from horses, cattle, pigs, sheep, and dogs, m Shinjiang, Jilin, Beijing, Fujian, and Tibet provinces, China, respectively, were examined for the diagnoses of babesiasis, toxoplasmosis, neosporosis, and cryptosrondiosis, and obtained many new findings.