Epidemiological investigation of protozoan diseases in China

我国原虫病流行病学调查

• 批准号: 13575030
• 负责人: XUAN Xuenan
• 金额: $ 8.32万
• 依托单位: Obihiro University of Agriculture and Veterinary Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13575030/
• 关键词: Epidemiology; recombinant antigens; T.gondii; N.canunim; C.parvum; B.caballi; B.equi; B.gibsoni; クリストスポリジウム感染症; ELISA; 中国新疆地区; 中国吉林地区

1)Cloning of genes encoding immunodominant antigens of protozoan parasitesIn the present study, many genes encoding irnmunodominat antigens of protozoan parasites were identified, cloned and characterized. The main genes are as follows : B.caballi P48, P134;B.equi EMA-1,EMA-2,P82;B.gibsoni P18,P29,P50;N.caninum SAG1,SRS2;T.gondii SAG1,SAG2,SRS1,ROP-1.2)Development of serodiagnostic methods for detection of antibodies to protozoan parasitesThe genes encoding immunodominant antigens were expressed in E.coli or insect cells, and then established the enzyme-linked immunosorbent assay, latex agglutination test, and immunochromatographic test with these recombinant antigens.3)Development of polymerase chain reactions for detection of protozoan parasitesThe primer sets based on genes described above were designed, and established PCR methods for detection of protozoan parasites.4)Epidemiological investigation of protozoan diseases in ChinaBlood samples collected from horses, cattle, pigs, sheep, and dogs, m Shinjiang, Jilin, Beijing, Fujian, and Tibet provinces, China, respectively, were examined for the diagnoses of babesiasis, toxoplasmosis, neosporosis, and cryptosrondiosis, and obtained many new findings.

1)原生动物寄生虫免疫优势抗原基因的克隆本研究对原生动物寄生虫免疫优势抗原基因进行了鉴定、克隆和鉴定。主要基因如下：卡氏布氏杆菌（B.caballi）P48、P134;马布氏杆菌（B.equi）EMA-1、EMA-2、P82;吉氏布氏杆菌（B.gibsoni）P18、P29、P50;犬奈瑟氏杆菌（N.caninum）SAG 1、SRS 2;弓形虫SAG 1、SAG 2、SRS 1、ROP-1.2）弓形虫抗体血清学诊断方法的建立将免疫优势抗原基因在大肠杆菌或昆虫细胞中表达，建立酶联免疫吸附试验，根据上述基因设计引物，建立了检测原虫的PCR方法。4）中国原虫病流行病学调查采集吉林省新江县、北京市、吉林省新江县、吉林省新江县、吉林省在福建、西藏等地分别进行了巴贝虫病、弓形虫病、新孢子虫病和隐孢子虫病的诊断，获得了许多新的发现。

项目成果

Chahan, B., et al.: "Serodiagnosis of Neospora caninum infection in cattle by ELISA with recombinant truncated NcSAG1"Vet.Parasitol.. 118・3-4. 177-185 (2003)

Chahan, B., et al.：“用重组截短的 NcSAG1 通过 ELISA 进行牛新孢子虫感染的血清诊断”Vet.Parasitol.. 118・3-4 (2003)。

Huang, X., et al.: "High-level expression and purification of a truncated EMA-2 of Babesia equi in Escherichia coli and its potential for immunodiagnosis."J.Clin.Microbiol.. 41・3. 1147-1151 (2003)

Huang, X., et al.：“马巴贝虫的截短 EMA-2 在大肠杆菌中的高水平表达和纯化及其免疫诊断潜力。”J.Clin.Microbiol.. 41・3。 2003）

Hirata, H., et al.: "Identification of a specific antigenic region of P82 of Babesia equi and its potential use in serodiagnosis"J.Clin.Microbiol.. 41・1. 547-551 (2003)

Hirata, H., et al.：“马巴贝虫 P82 的特定抗原区域的鉴定及其在血清诊断中的潜在用途”J.Clin.Microbiol.. 41・1 (2003)。

Xuan, X., et al.: "Detection of antibodies to Babesia equi in horses by a latex agglutination test using recombinant EMA-1."Clin.Diagn.Lab.Immunol.. 8・3. 645-646 (2002)

Xu, X., et al.：“通过使用重组 EMA-1 的乳胶凝集试验检测马体内马巴贝虫抗体”。Clin.Diagn.Lab.Immunol.. 8・3（2002）。

Huang, X., Xuan, X., Yokoyama, N., Xu, L., Suzuki, H., Sugimoto, C., Nagasawa, H., Fujisaki, K., Igarashi, I.: "High-level expression and purification of a truncated merozote antigen-2 of Babesia equi in Escherichia coli and its potential for immunodiagno

Huang, X., Xu, X., Yokoyama, N., Xu, L., Suzuki, H., Sugimoto, C., Nagasawa, H., Fujisaki, K., Igarashi, I.：“高级表达