Comparative epidemiological study on zoonotic diseases originating from wild rodents in Japan and Far East Russia

日本与俄罗斯远东地区野生啮齿类动物源性人畜共患病流行病学比较研究

• 批准号: 13575029
• 负责人: KARIWA Hiroaki
• 金额: $ 7.36万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13575029/
• 关键词: zoonosis; rodent; epidemiology; hemorrhagic fever with renal syndrome; tick-borne encephalitis

To know the epidemiology of viral zoonotic diseases such as hemorrhagic fever with renal syndrome (HFRS) and tick-borne encephalitis (TBE) in Far East Russia, we Conducted an epizootiological survey in Khabarovsk. Anti-hantavirus antibodies were detected in Apodemus.agrarius (5/47), and Clethrionomys rufocanus (7/40) among 102 small animals captured. Partial M segments were amplified from seropositive A.agrarius. The virus nucleotide sequence in G2 region (2695-2926nt) from A agrarius had 97.0 -99.6% identities with those of FE genotypes which were identified from blood samples of HFRS patients. On the contrary, the sequence had 80.7 to 82.5% identities with those of AMR. These results suggest that hantavirus FE genotype cases severe HFRS and is carried by A agrarius. We established the ELISA to detect anti-TBE virus antibodies by using the recombinant viral E protein expressed in E.coli expression system. The ELISA could detected anti-TBE virus antibodies in C.rufocanus captured in Khabarovsk, which were also positive by authentic neutralization test. This indicate that the ELISA can be a useful method to identify the endemic areas of TBE.

为了解俄罗斯远东地区肾综合征出血热（HFRS）、蜱传脑炎（TBE）等病毒性人畜共患疾病的流行病学，在哈巴罗夫斯克市进行了流行病学调查。在鼠体中检测到抗汉坦病毒抗体。在捕获的102只小动物中，黑线鼠（5/47）和红毛鼠（7/40）。从血清阳性黑线姬鼠中扩增出部分M片段。黑线绣线鼠G2区（2695 ~ 2926nt）的病毒核苷酸序列与HFRS患者血样中FE基因型的同源性为97.0% ~ 99.6%。与AMR序列的同源性为80.7% ~ 82.5%。结果表明，汉坦病毒FE基因型可引起严重的HFRS，由黑线姬鼠携带。利用大肠杆菌表达系统中表达的重组病毒E蛋白，建立了检测抗tbe病毒抗体的ELISA方法。ELISA能检测到在哈巴罗夫斯克捕获的猪棘球蚴的抗tbe病毒抗体，经真实中和试验也呈阳性。这表明ELISA可作为一种有效的方法来确定TBE的流行地区。

项目成果

Araki K, et al.: "A new model of Hantaan virus persistence in mice : the balance between HTNV infection and CD8+ T-cell responses."Virology. 322(2). 318-327 (2004)

Araki K 等人：“汉坦病毒在小鼠体内持续存在的新模型：HTNV 感染与 CD8 T 细胞反应之间的平衡。”病毒学。

Kariwa H.: "Epizootiological of hantavirus infections."BAMSA J.. 15. 16-22 (2004)

Kariwa H.：“汉坦病毒感染的流行病学。”BAMSA J.. 15. 16-22 (2004)

Araki K, Yoshimstsu K, Ogino M, Ebihara H, Lundkvist A, Kariwa H, Takashima I, Arikawa J.: "Truncatad hantavirus nucleocapsid proteins for serotyping Hantaan, Seoul, and Dobrava hantavirus lions."J Clin Microbiol.. 39. 2397-2404 (2001)

Araki K、Yoshimstsu K、Ogino M、Ebihara H、Lundkvist A、Kariwa H、Takashima I、Arikawa J.：“Truncatad 汉坦病毒核衣壳蛋白用于汉坦、首尔和多布拉瓦汉坦病毒狮子的血清分型。”J Clin Microbiol.. 39. 2397

Miyamoto H, Kariwa H, Araki K, Lokugamage K, Hayasaka O, Cui BZ, Lokugamage N, Ivanov LI, Mizutani T, Iwasa MA, Yoshimatsu K, Arikawa J, Takashima I.: "Serological analysis of hemorrhagic fever with renal syndrome (HFRS) patients in Far Eastern Russia end

Miyamoto H, Kariwa H, Araki K, Lokugamage K, Hayasaka O, Cui BZ, Lokugamage N, Ivanov LI, Mizutani T, Iwasa MA, Yoshimatsu K, Arikawa J, Takashima I.：“肾综合征出血热的血清学分析（

Kariwa H.: "Epidemiology of hantavirus infections."BAMSAJ.(In Japanese). 15. 16-22 (2004)

Kariwa H.：“汉坦病毒感染的流行病学。”BAMSAJ.（日语）。