Comparative epidemiological study on zoonotic diseases originating from wild rodents in Japan and Far East Russia

为了解俄罗斯远东地区肾综合征出血热（HFRS）和蜱传脑炎（TBE）等病毒性人兽共患疾病的流行病学情况，我们在哈巴罗夫斯克进行了动物流行病学调查。在102只捕获的小动物中，黑线姬鼠（5/47）和棕腹鼠（7/40）检出抗汉坦病毒抗体。部分M片段扩增从血清阳性A. agrarius。在G2区（2695- 2926 nt），黑线姬鼠病毒与HFRS患者血标本FE基因型的核苷酸同源性为97.0 - 99.6%。与AMR的同源性为80.7%~ 82.5%。这些结果表明，汉坦病毒FE基因型病例严重HFRS，并通过黑线姬鼠携带。利用大肠杆菌表达系统表达的重组病毒E蛋白，建立了检测TBE病毒抗体的ELISA方法。用ELISA法检测了从哈巴罗夫斯克捕获的褐口线虫中的TBE病毒抗体，真实中和试验也为阳性。说明ELISA法可作为结核杆菌病流行区的一种有效的鉴别方法。

To know the epidemiology of viral zoonotic diseases such as hemorrhagic fever with renal syndrome (HFRS) and tick-borne encephalitis (TBE) in Far East Russia, we Conducted an epizootiological survey in Khabarovsk. Anti-hantavirus antibodies were detected in Apodemus.agrarius (5/47), and Clethrionomys rufocanus (7/40) among 102 small animals captured. Partial M segments were amplified from seropositive A.agrarius. The virus nucleotide sequence in G2 region (2695-2926nt) from A agrarius had 97.0 -99.6% identities with those of FE genotypes which were identified from blood samples of HFRS patients. On the contrary, the sequence had 80.7 to 82.5% identities with those of AMR. These results suggest that hantavirus FE genotype cases severe HFRS and is carried by A agrarius. We established the ELISA to detect anti-TBE virus antibodies by using the recombinant viral E protein expressed in E.coli expression system. The ELISA could detected anti-TBE virus antibodies in C.rufocanus captured in Khabarovsk, which were also positive by authentic neutralization test. This indicate that the ELISA can be a useful method to identify the endemic areas of TBE.