Epidemiological study on viral zoonotic diseases originating from wild rodents in Japan

日本源自野生啮齿动物的病毒性人畜共患疾病流行病学研究

• 批准号: 13660311
• 负责人: KARIWA Hiroaki
• 金额: $ 2.3万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660311/
• 关键词: zoonosis; rodent; epidemiology; hemorrhagic fever with renal syndrome; tick-borne encephalitis; ダニ媒介脳炎ウイルス; ウイルス性脳炎; 病原性

To know the epidemiology of viral zoonotic diseases such as hemorrhagic fever with renal syndrome (HFRS) and tick-borne encephalitis (TBE) in Japan, we conducted an epizootiological survey in various points of Japan. Anti-hantavirus antibodies were detected in Apodemus. speciosus (5/482), and Clethnonomys rufocanus (7/197), Rattus norvegicus (4/364), and Rattus rattus (3/45) by Indirect fluorescent antibody assay (IFA). The positive sera from A.speciosus neutralized Hantaan virus at 1:20 but did not neutralize Seoul virus. This indicate that hantavirus carried by A.speciosus is closer to Hantaan virus than Seoul virus. The partial S gene was amplified from seropositive R.rattus and sequenced. The virus sequence had 96% identitiy with SR-11 which is Seoul virus prototype strain. These results suggest that main reservoir animals of hantavirus in Japan are A.speciosus, C. rufocanus, R.norvegicus, and R rattus.The rodent sera were applied to the newly established ELISA by using recombinant E protein of TBE virus to detected anti-TBE virus antibodies. The ELISA could detect anti-TBE antibodies in sera positive by neutralization test. However, no antibodies were detected in rodent sera from Toyama prefecture, in which TBE infections has not confirmed. These results suggest that the ELISA can be a useful method to identify the endemic areas of TBE in Japan.

为了解肾综合征出血热（HFRS）、蜱传脑炎（TBE）等病毒性人畜共患疾病在日本的流行病学情况，我们在日本各地进行了流行病学调查。在鼠体中检测到抗汉坦病毒抗体。间接荧光抗体测定法（IFA）检测到斑家鼠（5/482）、褐家鼠（7/197）、褐家鼠（4/364）和Rattus Rattus（3/45）。特异拟虫阳性血清在1:20能中和汉滩病毒，但不能中和汉城病毒。这说明拟虫携带的汉坦病毒比汉城病毒更接近汉坦病毒。从血清阳性鼠体中扩增部分S基因并测序。该病毒序列与汉城病毒原型株SR-11有96%的同源性。上述结果提示，日本汉坦病毒的主要宿主动物为特蠓、褐家鼠、褐家鼠和家鼠。将鼠血清应用于新建立的TBE病毒重组E蛋白酶联免疫吸附试验，检测TBE病毒抗体。ELISA可检测中和试验阳性血清中抗tbe抗体。然而，富山县的啮齿动物血清中未检测到抗体，该地区尚未确认TBE感染。结果表明，ELISA法可作为鉴定日本流行性乙型脑炎流行地区的有效方法。

项目成果

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Kariwa, H. et al.: "Epizootiological surrey of hantavirus among rodent species in Ningxia Hui Autonomou Province, China"Japanese Journal of Veterinary Research. 49.2. 105-114 (2001)

Kariwa, H. 等人：“中国宁夏回族自治省啮齿动物中汉坦病毒的流行病学调查”日本兽医研究杂志。

Lokugamage N, et al.: "Development of an efficient method for recovery of Puumala and Puumala-related viruses by inoculation of Mongolian gerbils"J Vet Med Sci.. 65(11). 1189-1194 (2003)

Lokugamage N 等人：“开发通过接种蒙古沙鼠来回收 Puumala 和 Puumala 相关病毒的有效方法”J Vet Med Sci. 65(11)。

Kariwa H.: "Bunyavirus-relationship between virus and host : Coevolutlon of hantavirus and rodent."Virus(In Japanese). 52. 61-67 (2002)

Kariwa H.：“布尼亚病毒-病毒与宿主之间的关系：汉坦病毒与啮齿动物的共同进化。”病毒（日语）。

Shirato K, et al.: "Discrimination of West Nile virus and Japanese encephalitis virus strains using RT-PCR RFLP analysis."Microbiol Immunol.. 47(6). 439-445 (2003)

Shirato K 等人：“使用 RT-PCR RFLP 分析区分西尼罗河病毒和日本脑炎病毒株。”微生物免疫学 47(6)。