Epidemiological study on viral zoonotic diseases originating from wild rodents in Japan

日本源自野生啮齿动物的病毒性人畜共患疾病流行病学研究

• 批准号: 13660311
• 负责人: KARIWA Hiroaki
• 金额: $ 2.3万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660311/
• 关键词: zoonosis; rodent; epidemiology; hemorrhagic fever with renal syndrome; tick-borne encephalitis; ダニ媒介脳炎ウイルス; ウイルス性脳炎; 病原性

To know the epidemiology of viral zoonotic diseases such as hemorrhagic fever with renal syndrome (HFRS) and tick-borne encephalitis (TBE) in Japan, we conducted an epizootiological survey in various points of Japan. Anti-hantavirus antibodies were detected in Apodemus. speciosus (5/482), and Clethnonomys rufocanus (7/197), Rattus norvegicus (4/364), and Rattus rattus (3/45) by Indirect fluorescent antibody assay (IFA). The positive sera from A.speciosus neutralized Hantaan virus at 1:20 but did not neutralize Seoul virus. This indicate that hantavirus carried by A.speciosus is closer to Hantaan virus than Seoul virus. The partial S gene was amplified from seropositive R.rattus and sequenced. The virus sequence had 96% identitiy with SR-11 which is Seoul virus prototype strain. These results suggest that main reservoir animals of hantavirus in Japan are A.speciosus, C. rufocanus, R.norvegicus, and R rattus.The rodent sera were applied to the newly established ELISA by using recombinant E protein of TBE virus to detected anti-TBE virus antibodies. The ELISA could detect anti-TBE antibodies in sera positive by neutralization test. However, no antibodies were detected in rodent sera from Toyama prefecture, in which TBE infections has not confirmed. These results suggest that the ELISA can be a useful method to identify the endemic areas of TBE in Japan.

为了了解日本病毒性人畜共患病的流行病学，例如肾脏综合征（HFRS）和tick传播的脑炎（TBE），我们在日本各个地方进行了epizootiologicy调查。在apodemus中检测到抗汉塔病毒抗体。 Speciosus（5/482）和Clethnonomys Rufocanus（7/197），Rattus Norvegicus（4/364）和Rattus Rattus（3/45）通过间接荧光抗体分析（IFA）。 A.seciosus的阳性血清在1:20中和汉塔病毒，但没有中和汉城病毒。这表明A.seciosus携带的汉坦病毒比汉塔病毒更接近汉塔病毒。部分S基因从血清阳性r.rattus中放大并进行了测序。该病毒序列与SR-11的识别为96％，SR-11是首尔病毒原型菌株。这些结果表明，日本汉塔病毒的主要储层动物是A.seciosus，C。rufocanus，R.Norvegicus和R rattus。通过使用TBE病毒的重组E蛋白来检测抗Tbe病毒抗体。 ELISA可以通过中和测试在血清中检测抗TBE抗体。但是，在富兰县的啮齿动物血清中未检测到抗体，其中尚未确认感染。这些结果表明，ELISA可能是确定日本特有领域的有用方法。

项目成果

Kariwa, H. et al.: "Epizootiological surrey of hantavirus among rodent species in Ningxia Hui Autonomou Province, China"Japanese Journal of Veterinary Research. 49.2. 105-114 (2001)

Kariwa, H. 等人：“中国宁夏回族自治省啮齿动物中汉坦病毒的流行病学调查”日本兽医研究杂志。

Kariwa H.: "Bunyavirus-relationship between virus and host : Coevolutlon of hantavirus and rodent."Virus(In Japanese). 52. 61-67 (2002)

Kariwa H.：“布尼亚病毒-病毒与宿主之间的关系：汉坦病毒与啮齿动物的共同进化。”病毒（日语）。

Shirato K, et al.: "Discrimination of West Nile virus and Japanese encephalitis virus strains using RT-PCR RFLP analysis."Microbiol Immunol.. 47(6). 439-445 (2003)

Shirato K 等人：“使用 RT-PCR RFLP 分析区分西尼罗河病毒和日本脑炎病毒株。”微生物免疫学 47(6)。

Araki, K. et al.: "Truncated hantavirus nucleocapsid proteins for serotyping Hantaan, Seoul and Dobrav hantavirus infection"Journal of Clinical Microbiology. 39.7. 2397-2904 (2001)

Araki, K. 等人：“截短的汉坦病毒核衣壳蛋白用于汉坦、首尔和多布拉夫汉坦病毒感染的血清分型”临床微生物学杂志。

Araki K, et al.: "A new model of Hantaan virus persistence in mice : the balance between HTNV infection and CD8+ T-cell responses."Virology. 322(2). 318-327 (2004)

Araki K 等人：“汉坦病毒在小鼠体内持续存在的新模型：HTNV 感染与 CD8 T 细胞反应之间的平衡。”病毒学。