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Epidemiological study on zoonotic diseases endemic in North America which are potentially introduced into Japan

北美流行的人畜共患病可能传入日本的流行病学研究

基本信息

批准号：
16405034
负责人：
KARIWA Hiroaki
金额：
$ 8.95万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16405034/
关键词：
West Nile fever West Nile virus Wild birds Hantavirus infection Hantavirus Rodents Epidemiology フラビウイルス感染症鳥類病原性診断法診断

项目摘要

In this study, new diagnostic methods were developed and the epidemiological surveys were carried out on West Nile fever and hantavirus infections which are potentially introduced into Japan.1) Development of diagnostic methods for West Nile fever: To establish the surveillance system to West Nile virus introduction to Japan, serological diagnostic methods for anti-West Nile virus antibodies were developed. The newly developed methods were the micro-neutralization test and inhibition ELISA.2) Epidemiological study of West Nile fever in wild birds in Far East Russia: Total RNA was extracted from kidneys of 98 birds captured in Far East Russia and West Nile virus RNA was tried to detect by RT-PCR. Although no West Nile virus RNA was detected, neutralizing antibodies to West Nile virus were detected in 15 bird sera out of 91 samples. Therefore, it is suggested that West Nile virus is circulating among wild birds in Far East Russia. Since a lot of wild birds migrate from Far East Russia to Japan, reinforcement of control measures to West Nile fever is highly required.3) Epidemiological study of hantavirus infections among rodents in Mexico: Virus genome detection method for hantaviruses existing in North America was developed by RT-PCR. Total RNA was extracted from lungs of 213 rodents captured in Mexico and hantavirus genome was tried to detect by RT-PCR. Among 213 samples, hantavirus RNAs were detected from 21 samples. Among these 21 virus-RNA positive rodents, 19 animals had anti-hantavirus antibodies. Therefore, it was revealed that hantaviruses are circulating among rodents in Mexico where the information of hantavirus infection is extremely limited.

在本研究中，开发了新的诊断方法，并对可能传入日本的西尼罗河热和汉坦病毒感染进行了流行病学调查。1）西尼罗河热诊断方法的开发：为了建立西尼罗河病毒传入日本的监测系统，开发了抗西尼罗河病毒抗体的血清学诊断方法。2）俄罗斯远东地区野鸟西尼罗热流行病学研究：从俄罗斯远东地区捕获的98只野鸟的肾脏中提取总RNA，尝试用RT-PCR方法检测西尼罗病毒RNA。虽然没有检测到西尼罗河病毒RNA，但在91份样品中的15份鸟类血清中检测到西尼罗河病毒的中和抗体。因此，有人认为西尼罗河病毒正在俄罗斯远东地区的野生鸟类中传播。由于大量野鸟从俄罗斯远东地区迁徙至日本，加强对西尼罗热的控制措施是非常必要的。3）墨西哥啮齿动物中汉坦病毒感染的流行病学研究：通过RT-PCR建立了北美汉坦病毒的病毒基因组检测方法。从墨西哥捕获的213只啮齿类动物的肺中提取总RNA，尝试用RT-PCR方法检测汉坦病毒基因组。在213份样本中，21份样本检测到汉坦病毒RNA。在这21只病毒-RNA阳性啮齿动物中，19只动物具有抗汉坦病毒抗体。因此，据透露，汉坦病毒是在墨西哥的啮齿动物之间传播，汉坦病毒感染的信息是非常有限的。