Study on their experimetation of gerbils living in China for infectious diseases.

对生活在中国的沙鼠进行传染病实验研究。

• 批准号: 13575039
• 负责人: ITO Mamoru
• 金额: $ 7.87万
• 依托单位: Central Institute for Experimental Animals
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13575039/
• 关键词: Gerbil; China; Experimentation; Model for infectious diseases; Immunology; Phylogeny

In order to investigate the potential use of Chinese gerbils as a laboratory animal model for human infectious diseases, we performed field surveys of wild rodents including gerbils living in the northwestern (Ily), southern (Quimo) and western (Kashu and Khotan) areas of the Xinjiang-Uygur autonomous regions of China three times between 2001 and 2003. In total, 340 small rodents of 10 species including gerbils were captured in the surveys, parasitological examinations of the rodents were performed, and fecal and DNA samples were also collected. PCR analysis using fecal samples revealed that Helicobacter spp. was widespread in wild rodents but H.pylori was not detected in any genera tested. Experimental infections of the captured gerbils with human pathogens, Helicobacter pylori, Trichinera spilaris, Echinococcus spp and Leishmania infantum were also performed to investigate sensitivity of infection in the gerbils. In order to clarify the phylogenic relationships among seven species of gerbils living in China, DNA sequences of parts of the mitochondrial cytochrome b and cylochrome c oxidase subunit II genes were determined and compared. These studies revealed that regional variations exist in Meriones meridianus and that M.chengi, which was considered as an independent species, was actually a synonym of M.meridianus. These DNA sequences registered in GenBank may contribute to the phylogenic study on gerbils world wide.

为了探讨中国沙鼠作为人类传染病实验动物模型的可能性，我们于2001 - 2003年对新疆维吾尔自治区西北部（伊犁）、南部（即墨）和西部（喀什和和田）的野生啮齿动物包括沙鼠进行了3次野外调查。调查共捕获沙鼠等10种340只小型啮齿动物，进行寄生虫学检查，并采集粪便和DNA样本。粪便样本PCR分析显示，幽门螺杆菌属。在野生啮齿类动物中广泛存在，但在任何测试属中均未检测到幽门螺杆菌。用幽门螺杆菌、旋毛虫、棘球蚴和婴儿利什曼原虫对捕获的沙鼠进行实验感染，以研究沙鼠感染的敏感性。为了探讨我国7种沙鼠的亲缘关系，测定并比较了线粒体细胞色素B和细胞色素c氧化酶亚基II基因部分序列。这些研究表明，沙鼠存在区域性变异，而被认为是一个独立种的程氏沙鼠实际上是沙鼠的异名。这些序列已在GenBank中登录，为进一步研究沙鼠的遗传学奠定了基础。

项目成果

Epidemiology of Hilicobacter Infection in Wild Rodents in the Xinjiang-Uygur Autonmous region of China.

中国新疆维吾尔自治区野生啮齿动物幽门螺杆菌感染流行病学。

• 发表时间: 2003
• 作者: Kazuo Goto

epidemiology of Hilicobacter Infection in Wild Rodents in the Xinjinag-Uygur Autonomous region of China

新疆维吾尔自治区野生啮齿动物幽门螺杆菌感染流行病学调查

• 发表时间: 2003
• 作者: Kazuo Goto;W.Jiang;Q.Zheng;Y.Oku;H.Kamiya;T.Itch;M.Ito
• 通讯作者: M.Ito

Characterization of SSU and LSU rRNA genes of three Trypanosoma (Herpetosoma) grosi isolates maintained in Mongolian jirds

• DOI: 10.1017/s0031182004006493
• 发表时间: 2004-10
• 期刊: Parasitology
• 影响因子: 2.4
• 作者: Hiroshi Sato;A. Osanai;H. Kamiya;Y. Obara;W. Jiang;Q. Zhen;J. Chai;Yumi Une;Mamoru Ito

Sato, H.: "Monoclonal antibodies reactive with dendritic cells of Mongolian gerbils"Comparative Medicine. 51. 234-238 (2001)

Sato, H.：“与蒙古沙鼠树突状细胞反应的单克隆抗体”比较医学。

Sato, H.: "Defect of protective immunity to Schistosoma mansoni infection in Mongolian gerbils involves limited recruitment of dendritic cells in the vaccinated skin"Parasite Immunology. 23. 627-632 (2001)

Sato, H.：“蒙古沙鼠对曼氏血吸虫感染的保护性免疫缺陷涉及接种疫苗的皮肤中树突状细胞的有限募集”寄生虫免疫学。