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Development of Chinese Lemming as an Experimental Animal

中国旅鼠实验动物的发展

基本信息

批准号：
10041210
负责人：
ITO Mamoru
金额：
$ 5.18万
依托单位：
Central Institute for Experimental Animals
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

Parasitological and virological surveys of wil rodents captured in the Xinjiang-Uygur autonomous region of China over 2 years from 1998 through 1999 were carried out. In order to develop new laboratory animal models for human infectious diseases, attempts were made to rear Lagurus lagurus and Meriones meridianus in the laboratory and to experimentally infect the laboratory-born animals with Echinococcus spp., Leishmania infantum and Cryptosporidium spp.A total of 569 wild small animals were captured in 17 localities in and around Mt. Tien-Shan and Mt. Altai and belonged to 2 families, 2 genera and 2 species of Insectivora, and 3 families, 18 genera, and 25 species of Rodentia. Twenty four species of acanthocephala and nematodes, and seven species of cestodes were obtained from the captured animals in parasitological examinations. Anti-Borna disease virus antibodies were detected in 4 out of 20 horses and 5 out of 20 sheep.In experimental infection of L.lagurus, M.meridianus and golden hamster with Echinococcus spp, M.meridianus was highly sensitive to both E.multilocularis and E.granulosus infections. Oocysts of Cryptosporidum purvum and C.muris were detected in feces from both L.lagurus and M.meridianus orally inoculated with Cryptosporidum spp. for 2 to 18 days after inoculation, indicating that these animals can be used as a new infection model for cryptosporidiosis. In experimental infection with L.infantum, the protozoa grew more rapidly in livers and spleens of L.lagurus than in those of hamsters.

对1998年至1999年两年间在中国新疆维吾尔自治区捕获的野生啮齿动物进行了寄生虫学和病毒学调查。为建立人类传染病新的实验动物模型，尝试在实验室饲养湖豚和子午线沙鼠，并对实验室出生的动物进行棘球绦虫、婴儿利什曼原虫、隐孢子虫感染实验。在天山、阿尔泰山及其周边17个地点共捕获野生小动物569只。食虫目2科2属2种，啮齿目3科18属25种。寄生虫学检查，从捕获的动物中获得棘头动物和线虫24种、绦虫7种。 20匹马中的4匹和20只羊中的5匹检测到抗博尔纳病病毒抗体。在用棘球绦虫感染L.lagurus、M.meridianus和金黄地鼠的实验中，M.meridianus对多房棘球绦虫和细粒棘球绦虫感染均高度敏感。在口服接种隐孢子虫属的 L.lagurus 和 M.meridianus 的粪便中检测到 Cryptosporidum purvum 和 C.muris 的卵囊。接种后持续2～18天，表明这些动物可作为隐孢子虫病新的感染模型。在婴儿乳杆菌的实验感染中，原生动物在兔乳鼠的肝脏和脾脏中的生长速度比仓鼠的更快。