Research for the genetic mechanism of reproductive isolation between two mouse subspecies

两个小鼠亚种生殖隔离的遗传机制研究

• 批准号: 14340239
• 负责人: SHIROISHI Toshihiko
• 金额: $ 8.9万
• 依托单位: National Institute of Genetics
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14340239/
• 关键词: hybrid breakdown; X chromosome; consomic strain; male sterility; spermatogenesis; 精子形態異常; 生殖能力; QTLマッピング; Halap-X遺伝子; 遺伝子多型; コンジェニック系統

Hybrid breakdown is a type of reproductive failure that appears after the F2 generation of crosses between different species or subspecies. It is caused by incompatibility between interacting genes. Genetic analysis of hybrid breakdown, particularly in higher animals : has been hampered by its complex nature (i.e., it involves more than two genes, and the phenotype is recessive). We studied hybrid breakdown using a new consomic strain, C57BU6J-X^<MSM> in which the X chromosome of C57BL/6J (derived mostly from Mus musculus domesticus) is substituted by the X chromosome of the MSM/Ms strain (M.m.molossinus). Males of this consomic strain are sterile, whereas F_1 hybrids between C57BU6J and MSM/Ms are completely fertile. The C57BU6J-X^<MSM> males showed reduced testis weight with variable defects in spermatogenesis and abnormal sperm head morphology. We conducted quantitative trait locus (QTL) analysis for these traits to map the X-linked genetic factors responsible for the sterility. This analysis successfully detected at least three distinct loci for the sperm head morphology and one for the testis weight. This study revealed that incompatibility of interactions of X-linked gene(s) with autosomal and/or Y-linked gene(s) causes the hybrid breakdown between the genetically distant C57BL/6J and MSM/Ms strains.

杂交失败是不同物种或亚种间杂交的F2代后出现的一种繁殖失败。它是由相互作用的基因之间的不相容性引起的。杂种破坏的遗传分析，特别是在高等动物中：由于其复杂的性质（即，它涉及两个以上的基因，并且表现型是隐性的）。我们用一个新的同源品系C57 BU 6 J-X ^研究了杂种的破坏<MSM>，其中C57 BL/6 J的X染色体（主要来自小家鼠）被MSM/Ms品系（M. m. molossinus）的X染色体所取代。C57 BU 6 J与MSM/Ms的杂种F_1完全可育。C57 BU 6 J-X ^<MSM>雄性动物显示睾丸重量减轻，精子发生不同程度缺陷，精子头部形态异常。我们对这些性状进行了数量性状位点（QTL）分析，以定位与不育有关的X连锁遗传因子。该分析成功地检测到至少三个不同的位点的精子头部形态和一个睾丸重量。本研究揭示了X连锁基因与常染色体和/或Y连锁基因的相互作用的不相容性导致遗传上远缘的C57 BL/6 J和MSM/Ms株之间的杂交失败。

项目成果

Oka, A.et al.: "Hybrid breakdown caused by substitution of X chromosome between two mouse subspecies"Genetics. 166. 913-924 (2004)

Oka, A. 等人：“两个小鼠亚种之间 X 染色体替换引起的杂交破坏”遗传学。

Oka, A.et al., Hybrid Breakdown Caused by: "Substitution of X Chromosome between Two Mouse Subspecies"Genetics. 166. 913-924 (2004)

Oka, A. 等人，杂交故障引起的：“两个小鼠亚种之间 X 染色体的替换”遗传学。

Kikkawa, Y.et al.: "Mutations in a new scaffold protein Sans cause deafness in Jackson shaker mice. Hum."Mol.Genet.. 12. 453-461 (2003)

Kikkawa, Y.等人：“一种新的支架蛋白 Sans 的突变导致杰克逊摇床小鼠耳聋。嗯。”Mol.Genet.. 12. 453-461 (2003)

Isobe, T.et al.: "Molecular characterization of the Pb recombination hotspot in the mouse major histocompatibility complex class II region"Genomics. 80. 229-235 (2002)

Isobe, T. 等人：“小鼠主要组织相容性复合体 II 类区域中 Pb 重组热点的分子特征”基因组学。

Hide, T.et al.: "Genetic modifiers of otocephalic phenotypes in Otx2 heterozygous mutant mice"Development. 129. 4347-4357 (2002)

Hide, T. 等人：“Otx2 杂合突变小鼠耳头表型的遗传修饰剂”开发。