Research for the genetic mechanism of reproductive isolation between two mouse subspecies

两个小鼠亚种生殖隔离的遗传机制研究

• 批准号: 14340239
• 负责人: SHIROISHI Toshihiko
• 金额: $ 8.9万
• 依托单位: National Institute of Genetics
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14340239/
• 关键词: hybrid breakdown; X chromosome; consomic strain; male sterility; spermatogenesis; 精子形態異常; 生殖能力; QTLマッピング; Halap-X遺伝子; 遺伝子多型; コンジェニック系統

Hybrid breakdown is a type of reproductive failure that appears after the F2 generation of crosses between different species or subspecies. It is caused by incompatibility between interacting genes. Genetic analysis of hybrid breakdown, particularly in higher animals : has been hampered by its complex nature (i.e., it involves more than two genes, and the phenotype is recessive). We studied hybrid breakdown using a new consomic strain, C57BU6J-X^<MSM> in which the X chromosome of C57BL/6J (derived mostly from Mus musculus domesticus) is substituted by the X chromosome of the MSM/Ms strain (M.m.molossinus). Males of this consomic strain are sterile, whereas F_1 hybrids between C57BU6J and MSM/Ms are completely fertile. The C57BU6J-X^<MSM> males showed reduced testis weight with variable defects in spermatogenesis and abnormal sperm head morphology. We conducted quantitative trait locus (QTL) analysis for these traits to map the X-linked genetic factors responsible for the sterility. This analysis successfully detected at least three distinct loci for the sperm head morphology and one for the testis weight. This study revealed that incompatibility of interactions of X-linked gene(s) with autosomal and/or Y-linked gene(s) causes the hybrid breakdown between the genetically distant C57BL/6J and MSM/Ms strains.

混合分解是一种生殖衰竭，在F2生成不同物种或亚种之间的杂交后出现。它是由相互作用基因之间的不兼容引起的。杂种分解的遗传分析，尤其是在高等动物中：它的复杂性质受到阻碍（即，它涉及两个以上的基因，表型是隐性的）。我们使用新的综合菌株C57BU6J-X^<msm>研究了混合分解，其中C57BL/6J的X染色体（主要来自use fordies useptialus）被MSM/MS菌株的X染色体取代（M.M.M.M.M.Molossinus）。这种杂质菌株的雄性是无菌的，而C57BU6J和MSM/MS之间的F_1杂种完全肥沃。 C57BU6J-X^<msm>男性显示出睾丸的体重降低，精子发生和精子形态异常的缺陷。我们对这些性状进行了定量性状基因座（QTL）分析，以绘制导致无菌性的X连锁遗传因素。该分析成功地检测到了精子形态的至少三个不同的基因座，另一种用于睾丸重量。这项研究表明，X连锁基因与常染色体和/或Y连锁基因的相互作用不兼容会导致遗传上远处的C57BL/6J和MSM/MS菌株之间的杂种分解。

项目成果

Oka, A.et al.: "Hybrid breakdown caused by substitution of X chromosome between two mouse subspecies"Genetics. 166. 913-924 (2004)

Oka, A. 等人：“两个小鼠亚种之间 X 染色体替换引起的杂交破坏”遗传学。

Oka, A.et al., Hybrid Breakdown Caused by: "Substitution of X Chromosome between Two Mouse Subspecies"Genetics. 166. 913-924 (2004)

Oka, A. 等人，杂交故障引起的：“两个小鼠亚种之间 X 染色体的替换”遗传学。

Kikkawa, Y.et al.: "Mutations in a new scaffold protein Sans cause deafness in Jackson shaker mice. Hum."Mol.Genet.. 12. 453-461 (2003)

Kikkawa, Y.等人：“一种新的支架蛋白 Sans 的突变导致杰克逊摇床小鼠耳聋。嗯。”Mol.Genet.. 12. 453-461 (2003)

Hide, T.et al.: "Genetic modifiers of otocephalic phenotypes in Otx2 heterozygous mutant mice"Development. 129. 4347-4357 (2002)

Hide, T. 等人：“Otx2 杂合突变小鼠耳头表型的遗传修饰剂”开发。

Isobe, T.et al.: "Molecular characterization of the Pb recombination hotspot in the mouse major histocompatibility complex class II region"Genomics. 80. 229-235 (2002)

Isobe, T. 等人：“小鼠主要组织相容性复合体 II 类区域中 Pb 重组热点的分子特征”基因组学。