Systematic genome function analysis based upon intersubspecific comsomic strains
基于亚种间体株的系统基因组功能分析
基本信息
- 批准号:16011258
- 负责人:
- 金额:$ 15.36万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research on Priority Areas
- 财政年份:2004
- 资助国家:日本
- 起止时间:2004 至 无数据
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We had developed a series of consomic mouse strains by substituting chromosomes of C57BL/6J strain by corresponding chromosomes of MSM/Ms strain. Because C57BL/6J originated from West European subspecies, Mus musculus domesticus, and MSM/Ms from Asian subspecies, M.m. molossinus, established strains should be referred to as intersubspecific consomic strains. By using these mouse strains, we are able to map loci controlling phenotypes that are different between C57BL/6J and MSM/Ms strains. In this study, we have further established experimental systems that enable us to explore the mouse genome functions, based upon phenotyping of the consomic strains. First, we developed a system for preservation of the strains as frozen sperms and embryos. Second, we found that X-chromosome consomic strain exhibits male sterility with abnormal morphology of sperms. We conducted QTL analysis, and successfully map three loci responsible for this male sterility. Third, in order to elucidate phenotypes that can be analyzed by the consomic strains, we have collected comprehensive, phenotype data of consomic parental stains, C57BL/6j and MSM/Ms. Finally, we executed phenotyping of all consomic strains for some of morphological traits including growth curve and behavioural traits, anxiety and home-cage activity. As a result, we could map a number of intriguing phenotypes.
我们用MSM/Ms品系的相应染色体取代C57 BL/6 J品系的染色体,建立了一系列的同源小鼠品系。由于C57 BL/6 J来源于西欧亚种Mus musculus sputicus,MSM/Ms来源于亚洲亚种M.m.对于molossinus,建立的菌株应称为亚种间同源菌株。通过使用这些小鼠品系,我们能够映射控制C57 BL/6 J和MSM/Ms品系之间不同的表型的基因座。在本研究中,我们进一步建立了实验系统,使我们能够探索小鼠基因组功能,基于consomic菌株的表型。首先,我们开发了一个系统,用于保存冷冻精子和胚胎的菌株。第二,X染色体同源株系表现雄性不育,精子形态异常。我们进行了QTL分析,并成功地定位了三个基因座负责这一雄性不育。第三,为了阐明可以分析的consomic菌株的表型,我们已经收集了全面的,consomic亲本菌株,C57 BL/6 j和MSM/MS的表型数据。最后,我们执行了所有consomic菌株的一些形态特征,包括生长曲线和行为特征,焦虑和家庭笼活动的表型。因此,我们可以绘制出许多有趣的表型。
项目成果
期刊论文数量(22)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Length variation of CAG/CAA triplet repeats in 50 genes among 16 inbred mouse strains
- DOI:10.1016/j.gene.2004.11.050
- 发表时间:2005-04-11
- 期刊:
- 影响因子:3.5
- 作者:Ogasawara, M;Imanishi, T;Koide, T
- 通讯作者:Koide, T
Hybrid Breakdown Caused by Substitution of X Chromosome between Two Mouse Subspecies.
两个小鼠亚种之间 X 染色体替换引起的杂交破坏。
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:Yoshii;T.;Funada;Y.;Ibuki;T.;Matsumoto;A.;Tanimura;T.;Tomioka;K.;Takehiro Kusakabe;Edgar A.Otto;Reiko Yoshida;Takehiro Kusakabe;Hironori Ando;日下部 岳広;Shiina T;International Chicken Genome Sequencing Consortium;Shiina T;Kulski JK;Hurt P;Shiina T;Ogasawara M.;Ogasawara M. et al.;Sakai T.;Oka A.
- 通讯作者:Oka A.
Phylogenic conservation of a cis-acting regulator that controls polarized expression of Sonic hedgehog (Shh) in limb buds.
控制肢芽中音刺猬 (Shh) 极化表达的顺式作用调节因子的系统发育保守性。
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:Yoshii;T.;Funada;Y.;Ibuki;T.;Matsumoto;A.;Tanimura;T.;Tomioka;K.;Takehiro Kusakabe;Edgar A.Otto;Reiko Yoshida;Takehiro Kusakabe;Hironori Ando;日下部 岳広;Shiina T;International Chicken Genome Sequencing Consortium;Shiina T;Kulski JK;Hurt P;Shiina T;Ogasawara M.;Ogasawara M. et al.;Sakai T.;Oka A.;Sagai T.
- 通讯作者:Sagai T.
Multivariate analysis of temporal descriptions of open-field behavior in wild-derived mouse strains
- DOI:10.1007/s10519-005-9038-3
- 发表时间:2006-09-01
- 期刊:
- 影响因子:2.6
- 作者:Takahashi, Aki;Kato, Katsunori;Koide, Tsuyoshi
- 通讯作者:Koide, Tsuyoshi
Ahl3, a third locus on mouse chromosome 17 affecting age-related hearing loss
- DOI:10.1016/j.bbrc.2004.09.186
- 发表时间:2004-11-26
- 期刊:
- 影响因子:3.1
- 作者:Nemoto, M;Morita, Y;Kominami, R
- 通讯作者:Kominami, R
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SHIROISHI Toshihiko其他文献
KOIDE Tsuyoshi Different patterns of ultrasonic vocalizations of wild-derived mouse strains from laboratory strains.
KOIDE Tsuyoshi 野生小鼠品系与实验室品系小鼠的超声波发声模式不同。
- DOI:
- 发表时间:
2009 - 期刊:
- 影响因子:0
- 作者:
SUGIMOTO Hiroki;KIKUSUI Takefumi;SHIROISHI Toshihiko - 通讯作者:
SHIROISHI Toshihiko
SHIROISHI Toshihiko的其他文献
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{{ truncateString('SHIROISHI Toshihiko', 18)}}的其他基金
Experimental evolutionary study of limb morphogenesis by a constitutive approach
本构法肢体形态发生的实验进化研究
- 批准号:
17K19411 - 财政年份:2017
- 资助金额:
$ 15.36万 - 项目类别:
Grant-in-Aid for Challenging Research (Exploratory)
Mutation burst by conflict of two mouse subspecies genomes
两个小鼠亚种基因组冲突导致突变爆发
- 批准号:
23657009 - 财政年份:2011
- 资助金额:
$ 15.36万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Gene regulation via dynamic change of chromosomal conformation of remote enhancers
通过远程增强子染色体构象的动态变化进行基因调控
- 批准号:
21247002 - 财政年份:2009
- 资助金额:
$ 15.36万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Genetic regulation of tissue-specific Shh expression by long-range enhancers and chromosome dynamics
长程增强子和染色体动力学对组织特异性Shh表达的遗传调控
- 批准号:
19370003 - 财政年份:2007
- 资助金额:
$ 15.36万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Functional Genomics based upon mouse inter-subspecific consomic strain
基于小鼠亚种间康体菌株的功能基因组学
- 批准号:
17018033 - 财政年份:2005
- 资助金额:
$ 15.36万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
Limb bud-specific Shh enhancer that is 1Mb apart from Shh coding sequence
肢芽特异性 Shh 增强子,距 Shh 编码序列 1Mb
- 批准号:
17370003 - 财政年份:2005
- 资助金额:
$ 15.36万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Research for the genetic mechanism of reproductive isolation between two mouse subspecies
两个小鼠亚种生殖隔离的遗传机制研究
- 批准号:
14340239 - 财政年份:2002
- 资助金额:
$ 15.36万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Mous Saturation mutagenesis project:Construction of sperm bank for chromosome deletion mutants and development of screening system for the mutations
小鼠饱和诱变项目:染色体缺失突变体精子库建设及突变筛选系统开发
- 批准号:
10358019 - 财政年份:1998
- 资助金额:
$ 15.36万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Positional cloning of mouse morphological mutants
小鼠形态突变体的定位克隆
- 批准号:
09440257 - 财政年份:1997
- 资助金额:
$ 15.36万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Establishment of Mouse Consomic Lines
小鼠体系的建立
- 批准号:
07558116 - 财政年份:1995
- 资助金额:
$ 15.36万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
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