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Systematic genome function analysis based upon intersubspecific comsomic strains

基于亚种间体株的系统基因组功能分析

基本信息

批准号：
16011258
负责人：
SHIROISHI Toshihiko
金额：
$ 15.36万
依托单位：
National Institute of Genetics
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas
财政年份：
2004
资助国家：
日本
起止时间：
2004 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16011258/
关键词：
wild mouse consomic strains frozen sample preservation male sterility QTL analysis phenotype chromosome mapping マウス MSM Ms系統

项目摘要

We had developed a series of consomic mouse strains by substituting chromosomes of C57BL/6J strain by corresponding chromosomes of MSM/Ms strain. Because C57BL/6J originated from West European subspecies, Mus musculus domesticus, and MSM/Ms from Asian subspecies, M.m. molossinus, established strains should be referred to as intersubspecific consomic strains. By using these mouse strains, we are able to map loci controlling phenotypes that are different between C57BL/6J and MSM/Ms strains. In this study, we have further established experimental systems that enable us to explore the mouse genome functions, based upon phenotyping of the consomic strains. First, we developed a system for preservation of the strains as frozen sperms and embryos. Second, we found that X-chromosome consomic strain exhibits male sterility with abnormal morphology of sperms. We conducted QTL analysis, and successfully map three loci responsible for this male sterility. Third, in order to elucidate phenotypes that can be analyzed by the consomic strains, we have collected comprehensive, phenotype data of consomic parental stains, C57BL/6j and MSM/Ms. Finally, we executed phenotyping of all consomic strains for some of morphological traits including growth curve and behavioural traits, anxiety and home-cage activity. As a result, we could map a number of intriguing phenotypes.

我们用MSM/Ms品系的相应染色体取代C57 BL/6 J品系的染色体，建立了一系列的同源小鼠品系。由于C57 BL/6 J来源于西欧亚种Mus musculus sputicus，MSM/Ms来源于亚洲亚种M.m.对于molossinus，建立的菌株应称为亚种间同源菌株。通过使用这些小鼠品系，我们能够映射控制C57 BL/6 J和MSM/Ms品系之间不同的表型的基因座。在本研究中，我们进一步建立了实验系统，使我们能够探索小鼠基因组功能，基于consomic菌株的表型。首先，我们开发了一个系统，用于保存冷冻精子和胚胎的菌株。第二，X染色体同源株系表现雄性不育，精子形态异常。我们进行了QTL分析，并成功地定位了三个基因座负责这一雄性不育。第三，为了阐明可以分析的consomic菌株的表型，我们已经收集了全面的，consomic亲本菌株，C57 BL/6 j和MSM/MS的表型数据。最后，我们执行了所有consomic菌株的一些形态特征，包括生长曲线和行为特征，焦虑和家庭笼活动的表型。因此，我们可以绘制出许多有趣的表型。

项目成果

期刊论文数量（22）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Length variation of CAG/CAA triplet repeats in 50 genes among 16 inbred mouse strains

DOI：
10.1016/j.gene.2004.11.050
发表时间：
2005-04-11
期刊：
GENE
影响因子：
3.5
作者：
Ogasawara, M;Imanishi, T;Koide, T
通讯作者：
Koide, T

Hybrid Breakdown Caused by Substitution of X Chromosome between Two Mouse Subspecies.

两个小鼠亚种之间 X 染色体替换引起的杂交破坏。

DOI：
发表时间：
2004
期刊：
Genetics 166
影响因子：
0
作者：
Yoshii;T.;Funada;Y.;Ibuki;T.;Matsumoto;A.;Tanimura;T.;Tomioka;K.;Takehiro Kusakabe;Edgar A.Otto;Reiko Yoshida;Takehiro Kusakabe;Hironori Ando;日下部岳広;Shiina T;International Chicken Genome Sequencing Consortium;Shiina T;Kulski JK;Hurt P;Shiina T;Ogasawara M.;Ogasawara M. et al.;Sakai T.;Oka A.
通讯作者：
Oka A.

Phylogenic conservation of a cis-acting regulator that controls polarized expression of Sonic hedgehog (Shh) in limb buds.

控制肢芽中音刺猬 (Shh) 极化表达的顺式作用调节因子的系统发育保守性。

DOI：
发表时间：
2004
期刊：
Mamm. Genome 15
影响因子：
0
作者：
Yoshii;T.;Funada;Y.;Ibuki;T.;Matsumoto;A.;Tanimura;T.;Tomioka;K.;Takehiro Kusakabe;Edgar A.Otto;Reiko Yoshida;Takehiro Kusakabe;Hironori Ando;日下部岳広;Shiina T;International Chicken Genome Sequencing Consortium;Shiina T;Kulski JK;Hurt P;Shiina T;Ogasawara M.;Ogasawara M. et al.;Sakai T.;Oka A.;Sagai T.
通讯作者：
Sagai T.

Multivariate analysis of temporal descriptions of open-field behavior in wild-derived mouse strains