Systematic genome function analysis based upon intersubspecific comsomic strains

基于亚种间体株的系统基因组功能分析

• 批准号: 16011258
• 负责人: SHIROISHI Toshihiko
• 金额: $ 15.36万
• 依托单位: National Institute of Genetics
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16011258/
• 关键词: wild mouse; consomic strains; frozen sample preservation; male sterility; QTL analysis; phenotype; chromosome mapping; マウス; MSM; Ms系統

We had developed a series of consomic mouse strains by substituting chromosomes of C57BL/6J strain by corresponding chromosomes of MSM/Ms strain. Because C57BL/6J originated from West European subspecies, Mus musculus domesticus, and MSM/Ms from Asian subspecies, M.m. molossinus, established strains should be referred to as intersubspecific consomic strains. By using these mouse strains, we are able to map loci controlling phenotypes that are different between C57BL/6J and MSM/Ms strains. In this study, we have further established experimental systems that enable us to explore the mouse genome functions, based upon phenotyping of the consomic strains. First, we developed a system for preservation of the strains as frozen sperms and embryos. Second, we found that X-chromosome consomic strain exhibits male sterility with abnormal morphology of sperms. We conducted QTL analysis, and successfully map three loci responsible for this male sterility. Third, in order to elucidate phenotypes that can be analyzed by the consomic strains, we have collected comprehensive, phenotype data of consomic parental stains, C57BL/6j and MSM/Ms. Finally, we executed phenotyping of all consomic strains for some of morphological traits including growth curve and behavioural traits, anxiety and home-cage activity. As a result, we could map a number of intriguing phenotypes.

我们用MSM/MS株的相应染色体取代了C57BL/6J株的染色体，培育出了一系列共生小鼠品系。因为C57BL/6J起源于西欧亚种小鼠，而MSM/MS起源于亚洲亚种M.M.因此，已建立的品系应称为亚种间共生品系。通过使用这些小鼠品系，我们能够定位C57BL/6J和MSM/MS品系之间不同的表型控制基因座。在这项研究中，我们进一步建立了实验系统，使我们能够探索小鼠基因组功能，基于共体株的表型。首先，我们开发了一种保存冷冻精子和胚胎等菌株的系统。其次，我们发现X染色体共体品系表现出雄性不育，精子形态异常。我们进行了QTL分析，成功定位了3个与该雄性不育有关的基因座。第三，为了阐明可以被共生菌株分析的表型，我们收集了共生双亲菌株C57BL/6J和MSM/MS的全面的表型数据。最后，我们对所有共生菌株进行了一些形态特征的表型分析，包括生长曲线和行为特征，焦虑和在家笼子里的活动。结果，我们可以绘制出许多有趣的表型。

项目成果

Length variation of CAG/CAA triplet repeats in 50 genes among 16 inbred mouse strains

• DOI: 10.1016/j.gene.2004.11.050
• 发表时间: 2005-04-11
• 期刊: GENE
• 影响因子: 3.5
• 作者: Ogasawara, M;Imanishi, T;Koide, T
• 通讯作者: Koide, T

Hybrid Breakdown Caused by Substitution of X Chromosome between Two Mouse Subspecies.

两个小鼠亚种之间 X 染色体替换引起的杂交破坏。

• 发表时间: 2004
• 期刊: Genetics 166
• 作者: Yoshii;T.;Funada;Y.;Ibuki;T.;Matsumoto;A.;Tanimura;T.;Tomioka;K.;Takehiro Kusakabe;Edgar A.Otto;Reiko Yoshida;Takehiro Kusakabe;Hironori Ando;日下部 岳広;Shiina T;International Chicken Genome Sequencing Consortium;Shiina T;Kulski JK;Hurt P;Shiina T;Ogasawara M.;Ogasawara M. et al.;Sakai T.;Oka A.
• 通讯作者: Oka A.

Phylogenic conservation of a cis-acting regulator that controls polarized expression of Sonic hedgehog (Shh) in limb buds.

控制肢芽中音刺猬 (Shh) 极化表达的顺式作用调节因子的系统发育保守性。

• 发表时间: 2004
• 期刊: Mamm. Genome 15
• 作者: Yoshii;T.;Funada;Y.;Ibuki;T.;Matsumoto;A.;Tanimura;T.;Tomioka;K.;Takehiro Kusakabe;Edgar A.Otto;Reiko Yoshida;Takehiro Kusakabe;Hironori Ando;日下部 岳広;Shiina T;International Chicken Genome Sequencing Consortium;Shiina T;Kulski JK;Hurt P;Shiina T;Ogasawara M.;Ogasawara M. et al.;Sakai T.;Oka A.;Sagai T.
• 通讯作者: Sagai T.

Multivariate analysis of temporal descriptions of open-field behavior in wild-derived mouse strains

• DOI: 10.1007/s10519-005-9038-3
• 发表时间: 2006-09-01
• 期刊: BEHAVIOR GENETICS
• 影响因子: 2.6
• 作者: Takahashi, Aki;Kato, Katsunori;Koide, Tsuyoshi
• 通讯作者: Koide, Tsuyoshi

Origins of mouse inbred strains deduced from whole-genome scanning by polymorphic microsatellite loci

• DOI: 10.1007/s00344-004-3013-9
• 发表时间: 2005-01-01
• 期刊: MAMMALIAN GENOME
• 影响因子: 2.5
• 作者: Sakai, T;Kikkawa, Y;Yonekawa, H
• 通讯作者: Yonekawa, H