Analysis of the mechanisms of discrimination, recognition and response to food proteins by the immune system
免疫系统对食物蛋白的辨别、识别和反应机制分析
基本信息
- 批准号:14360070
- 负责人:
- 金额:$ 8.58万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2002
- 资助国家:日本
- 起止时间:2002 至 2004
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1.The characteristics of orally tolerized T cells and the molecular mechanisms of their hyporesponsiveness was investigated. We elucidated the following characteristics of orally tolerant T cells. As a consequence of caspase activation, orally tolerant T cells could not form normal TCR signaling complexes associated with GADS-SLP-76. Orally-tolerized T cells showed defects in the translocation of TCR, PKC-θ, and lipid rafts into the interface between T cells and APCs, as well as impairment in vav activation. Thus orally-tolerized T cells were defective in immunological synapse formation. Furthermore, the expression of the protein phosphatase SHP-1 was upregulated in orally tolerized T cells. These observations may account for the hyporesponsive state of orally-tolerized T cells. We also found that T cells from orally tolerant mice could be divided into two populations based on the expression of CD44/CD62L, CD62L^<high>CD44^<low> cells and CD62L^<low>CD44^<high> cells. These cell populations were both hyporesponsive and possessed immunoregulatory functions. Nevertheless, they differed in cytokine secreting capacity and mode of suppression. The results suggested that oral administration of antigen induced two distinct types of hyporesponsive T cells with immunoregulatory function, that could be distinguished by expression of these cell surface molecules.2.Mice were orally administered with biotinylated antigen and antigen presentation was detected by fluorescence-labeled avidin. The results suggested that B220^+ Peyer's patch dendritic cells presented oral antigen.3.Germ-free T cell receptor transgenic mice were established and analyzed. It was shown that commensal bacteria affected the T cell cytokine response in the intestinal immune system.
1.研究了口服耐受性T细胞的特性及其低反应性的分子机制。我们阐明了口服耐受性T细胞的以下特征。作为半胱天冬酶活化的结果,口服耐受性T细胞不能形成与GADS-SLP-76相关的正常TCR信号传导复合物。口服耐受的T细胞在TCR、PKC-θ和脂筏易位到T细胞与APC之间的界面中方面表现出缺陷,以及vav活化的损伤。因此,口服耐受的T细胞在免疫突触形成中有缺陷。此外,蛋白磷酸酶SHP-1的表达上调口服耐受的T细胞。这些观察结果可以解释口服耐受的T细胞的低应答状态。我们还发现口服耐受小鼠的T细胞可以根据CD 44/CD 62 L的表达分为两个群体,CD 62 L ^<high>CD 44 ^<low>细胞和CD 62 L ^<low>CD 44 ^<high>细胞。这些细胞群都是低反应的,并具有免疫调节功能。然而,它们的细胞因子分泌能力和抑制方式不同。结果表明,经口给予抗原可诱导两种不同类型的具有免疫调节功能的低反应性T细胞,这两种低反应性T细胞可以通过细胞表面分子的表达来区分。2.经口给予生物素化抗原,用荧光标记的亲和素检测抗原提呈。结果表明,B220^+派伊尔集合淋巴结树突状细胞可提呈口服抗原。3.建立了无菌T细胞受体转基因小鼠,并进行了分析。结果表明,肠道细菌影响肠道免疫系统中的T细胞细胞因子应答。
项目成果
期刊论文数量(87)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
T.Nagura, et al.: "Suppressive Effect of Dietary Raffinose on T-helper 2 cell-mediated immunity"Br.J.Nutr.. 88. 421-426 (2002)
T.Nagura 等人:“膳食棉子糖对 T 辅助细胞 2 细胞介导的免疫的抑制作用”Br.J.Nutr.. 88. 421-426 (2002)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Identification of the genes specifically expressed in orally tolerized T cells.
鉴定在口服耐受 T 细胞中特异性表达的基因。
- DOI:
- 发表时间:2003
- 期刊:
- 影响因子:0
- 作者:T.Gotoh;et al.
- 通讯作者:et al.
T cell hyporesponsiveness induced by oral administration of ovalbumin is associated with impaired NFAT nuclear translocation and p27kip1 degradation.
口服卵清蛋白引起的 T 细胞反应低下与 NFAT 核易位受损和 p27kip1 降解有关。
- DOI:
- 发表时间:2002
- 期刊:
- 影响因子:0
- 作者:K.Asai;et al.
- 通讯作者:et al.
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HACHIMURA Satoshi其他文献
米胚乳タンパク質がTh1/Th2バランスに与える影響
水稻胚乳蛋白对Th1/Th2平衡的影响
- DOI:
- 发表时间:
2015 - 期刊:
- 影响因子:0
- 作者:
KOTAKI Ryutaro;SHIOKAWA Aya;HACHIMURA Satoshi;鎌田拓也・寺尾怜史・原 崇・久保田真敏・大谷 元・加藤久典・藤井幹夫・藤村 忍・門脇基二 - 通讯作者:
鎌田拓也・寺尾怜史・原 崇・久保田真敏・大谷 元・加藤久典・藤井幹夫・藤村 忍・門脇基二
Dendritic cells in mesenteric lymph nodes are divided into four subsets based on CD11b, CD103 and PD-1 expression
肠系膜淋巴结中的树突状细胞根据 CD11b、CD103 和 PD-1 表达分为四个亚群
- DOI:
- 发表时间:
2014 - 期刊:
- 影响因子:0
- 作者:
KOTAKI Ryutaro;SHIOKAWA Aya;HACHIMURA Satoshi - 通讯作者:
HACHIMURA Satoshi
Mesenteric lymph node CD11b-CD103+PD-1+ dendritic cells highly induce Foxp3+ T cells
肠系膜淋巴结 CD11b-CD103 PD-1 树突状细胞高度诱导 Foxp3 T 细胞
- DOI:
- 发表时间:
2015 - 期刊:
- 影响因子:0
- 作者:
KOTAKI Ryutaro;SHIOKAWA Aya;HACHIMURA Satoshi - 通讯作者:
HACHIMURA Satoshi
病原性の異なるマツノザイセンチュウを接種したマツ切枝における通水阻害
接种不同致病性松树线虫对松枝断枝水流的抑制作用
- DOI:
- 发表时间:
2008 - 期刊:
- 影响因子:0
- 作者:
MURAKAMI Hitoshi;HACHIMURA Satoshi;TANABE Kosuke;ADACHI(NAKAJIMA)Haruyo;TSUDA Masato;WAKATSUKI Yoshio;SATO Ryuichiro;TAKAHASHI Kyoko;HOSONO Akira;KAMINOGAWA Shuichi;外岡遼・梅林利弘・福田健二 - 通讯作者:
外岡遼・梅林利弘・福田健二
Functional differences in two different antigen specific T cell subsets induced in oral tolerance
口服耐受诱导的两种不同抗原特异性 T 细胞亚群的功能差异
- DOI:
- 发表时间:
2015 - 期刊:
- 影响因子:0
- 作者:
SHIBAHARA Kyoko;SHIOKAWA Aya;HOSONO Akira;NAKAJIMA-ADACHI Haruyo;HACHIMURA Satoshi - 通讯作者:
HACHIMURA Satoshi
HACHIMURA Satoshi的其他文献
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{{ truncateString('HACHIMURA Satoshi', 18)}}的其他基金
Regulation of chronic inflammation in aging and adipose tissues via the intestinal immune system by food
食物通过肠道免疫系统调节衰老和脂肪组织中的慢性炎症
- 批准号:
18H02152 - 财政年份:2018
- 资助金额:
$ 8.58万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Immunomodulation by foods based on elucidation of novel interactions of intestinal immune cells
基于肠道免疫细胞新相互作用的食物免疫调节
- 批准号:
26292065 - 财政年份:2014
- 资助金额:
$ 8.58万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Establishment of the next-generation evaluation system for immunomodulating functions of food using intestinal dendritic cells
利用肠道树突状细胞建立新一代食品免疫调节功能评价体系
- 批准号:
22658041 - 财政年份:2010
- 资助金额:
$ 8.58万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Elucidation of immunoregulatory function of intestinal immunoregulatory cells and its application to anti-infectious and anti-allergic food
肠道免疫调节细胞免疫调节功能的阐明及其在抗感染、抗过敏食品中的应用
- 批准号:
20380074 - 财政年份:2008
- 资助金额:
$ 8.58万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Elucidation of functional mechanisms of intestinal immune regulatory cells and recovery of immune function by foods using these cells as targets
阐明肠道免疫调节细胞的功能机制以及以这些细胞为靶点的食品恢复免疫功能
- 批准号:
17380076 - 财政年份:2005
- 资助金额:
$ 8.58万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
ELUCIDATION OF THE MOLECULAR MECHANISMS OF THE INTESTINAL IMMUNE RESPONSE TO FOOD PROTEIN ANTIGENS
阐明肠道对食物蛋白抗原免疫反应的分子机制
- 批准号:
12660110 - 财政年份:2000
- 资助金额:
$ 8.58万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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