Studies on Cyanobacterial Cyderophores
蓝藻细胞细胞的研究
基本信息
- 批准号:14360120
- 负责人:
- 金额:$ 9.47万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2002
- 资助国家:日本
- 起止时间:2002 至 2003
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Cyanobacteria are the prokaryotic phytoplankton and the representative primary producer in aquatic environment. They perform oxygenic photosynthesis using various iron-containing catalysis, and many species of them can also fix nitrogen by the iron-rich enzyme, nitrogenase. However little is known about the molecular mechanism to acquire the iron of cyanobacteria. In this report, I reported the first genuine Cyanobacterial siderophores from the freshwater cyanobacteria with nitrogen fixation ability.1. Screening for siderophore productive activity of various freshwater Cyanobacterial speciesFor large-scale screening, 41 axenic strains of freshwater cyanobacteria were selected and obtained from four culture collections, NIES, PCC, UTEX and IAM. In this screening, all strains of Microcystis aeruginosa did not show siderophore productive activity. The other noticeable result in this screening was that most of heterocystous diazotrophic species produced siderophores. This result gave me a … More hypothesis that heterocystous species had the faculty of producing siderophores to maintain their nitrogenase, an iron-rich protein, for nitrogen fixation.2. Anachelins, novel siderophores produced by Anabaena cylindrica NIES-19Novel Cyanobacterial siderophores, Anachelin-2 and Anachelin were isolated from Anabaena cylindrica NIES-19. The molecular formula of these compounds were determined to be C35H49013N6 from HRFAB-MS analysis. These structures were decided 2D NMR analyses including COSY, HMQC, 1H-13C HMBC, and 1H-15N HMBC experiments.3. Schizokinen, a siderophore produced by two strains of Anabaena variabilis M-204 and NIES-23Isolated siderophores from A. variabilis(M-204, NIES-23) were also positive in the Csaky test, indicating that this compound was a hydroxamate-type siderophore, schizokinen. The structure of schizokinen was confirmed by NMR analysis.4. Oscillabactin, a novel siderophore produced by Oscillatoria tenuis UTEX1566We isolated a novel Cyanobacterial siderophore, oscillabactin from Oscillatoria tenuis UTEX 1566. Oscillabactin is the first siderophore containing the hydroxypyridinone structure as the iron-binding group. Less
蓝藻是原核浮游植物,是水环境中具有代表性的初级生产者。它们利用各种含铁催化进行氧合光合作用,其中许多物种还可以通过富含铁酶-固氮酶来固定氮。然而,人们对蓝藻获取铁的分子机制知之甚少。在这份报告中,我报告了第一个真正的蓝藻铁载体,来自具有固氮能力的淡水蓝藻1。从NIES、PCC、UTEX和IAM 4种培养物中筛选出41株无菌淡水蓝藻进行大规模筛选。在本次筛选中,所有铜绿微囊藻菌株均未表现出产铁载体的活性。这次筛选的另一个值得注意的结果是,大多数异晶重氮营养物种都产生了铁载体。这个结果给了我一个…更多的假设是异形晶体物种有能力产生铁载体来维持它们的固氮酶,这是一种富含铁的蛋白质,用于固氮。从柱状鱼腥藻NIES-19中分离得到一种新型铁载体--鱼腥草素、鱼腥草素-2和鱼腥草素。经HRFAB-MS分析,确定其分子式为C35H49013N6。这些结构经COSY、HMQC、1H-13C HMBC和1H-15N HMBC实验等二维核磁共振分析确定。变异鱼腥藻M-204和NIES-23两株菌株产生的铁载体Schizokinen在Csaky试验中也呈阳性,表明该化合物为羟甲酸型铁载体。通过核磁共振谱确证了五味子激肽的结构。Oscillatoria tenuis UTEX1566产生的新铁载体Oscillabactin我们从Oscillatoria tenuis UTEX 1566中分离到一个新的蓝藻铁载体Oscillabactin。Oscillabactin是第一个以羟基吡啶酮为铁结合基团的铁载体。较少
项目成果
期刊论文数量(6)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
伊藤裕才, 村上昌弘: "藍藻類のユニークなシデロフォア-修飾された脂肪酸やキノリンアルカロイドを含む点に注目-"化学と生物. 41. 289-291 (2003)
Yusai Ito、Masahiro Murakami:“蓝绿藻的独特铁载体 - 以含有改性脂肪酸和喹啉生物碱而闻名”,《化学与生物学》41. 289-291 (2003)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Y.Itou etc: "Cyanobacterial siderophores : The chemical property and the physiological role"Fisheries Science. 68. 645-646 (2002)
Y.Itou等:“蓝藻铁载体:化学性质和生理作用”水产科学。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Y.Itou etc.: "Cyanobacterial siderophores : The chemical property and the physiological role"Fisheries Science. 68. 645-646 (2002)
Y.Itou等:“蓝藻铁载体:化学性质和生理作用”水产科学。
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- 发表时间:
- 期刊:
- 影响因子:0
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MURAKAMI Masahiro其他文献
MURAKAMI Masahiro的其他文献
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{{ truncateString('MURAKAMI Masahiro', 18)}}的其他基金
Organic Transformations Exploiting Solar Energy as the Driving Force
利用太阳能作为驱动力的有机转型
- 批准号:
24655077 - 财政年份:2012
- 资助金额:
$ 9.47万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Developement of New Synthetic Methods for Nitrogen-containing Compounds
含氮化合物合成新方法的开发
- 批准号:
23350041 - 财政年份:2011
- 资助金额:
$ 9.47万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Study of heat satability mechanism of 5'-AMPdeaminase in dried Iwa-nori
干岩海苔5-AMP脱氨酶热稳定性机制研究
- 批准号:
20580224 - 财政年份:2008
- 资助金额:
$ 9.47万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Activation of Carbon-Carbon Bonds by Transition Metals
过渡金属对碳-碳键的活化
- 批准号:
19205013 - 财政年份:2007
- 资助金额:
$ 9.47万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
In vivo 3 dimensional cartilage tissue engineering by adipose derived stem cells
利用脂肪干细胞进行体内 3 维软骨组织工程
- 批准号:
15591907 - 财政年份:2003
- 资助金额:
$ 9.47万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Insertion of Unsaturarel Organic Compounds into Carbon-Carbon Bonds
将不饱和有机化合物插入碳-碳键
- 批准号:
12450362 - 财政年份:2000
- 资助金额:
$ 9.47万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Utilization of antialgal compounds produced by lyticbacteria of cyanobacteria
蓝藻溶菌菌产生的抗藻化合物的利用
- 批准号:
12460090 - 财政年份:2000
- 资助金额:
$ 9.47万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
ANALYSIS OF THE LYTIC MECHANISM OF FILLAMENTOUS CYANOBACTERIA BY THE LITIC BACTERIA
细菌裂解丝状蓝藻的机理分析
- 批准号:
10460092 - 财政年份:1998
- 资助金额:
$ 9.47万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Studies on search of protease inhibitors in blue-green algae and biosynthetic pathways
蓝绿藻蛋白酶抑制剂的寻找及生物合成途径研究
- 批准号:
05453172 - 财政年份:1993
- 资助金额:
$ 9.47万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Studies on Biosynthesis of Bioactive Compounds produced by Microalgae
微藻生物活性化合物的生物合成研究
- 批准号:
03660206 - 财政年份:1991
- 资助金额:
$ 9.47万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)