Studies on Biosynthesis of Bioactive Compounds produced by Microalgae

微藻生物活性化合物的生物合成研究

• 批准号: 03660206
• 负责人: MURAKAMI Masahiro
• 金额: $ 1.28万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03660206/
• 关键词: Dinoflagellate; Alexandrium hiranoi; Goniodomin A; Polyether macrolide; Biosynthesis; Stable isotope; Microalgae; Goniodomin A; 抗カビ物質

Biosynthesis of polyether macrolide goniodomin A, which was produced by dinoflagellate Alexandrium hiranoi, was investigated by feeding experiments. Feeding experiments were carried out by adding [1-^<13>C_1],[2-^<13>C_1], or [1,2-^<13>C_2]-sodium acetate, Methyl- ^<13>C-methionine, and [1-^<13>C_1]-glucose of cultures of Alexandrium hiranoi. ^<13>C NMR signals of goniodomin A were assigned by analyses of 1D and 2D NMR spectra. Out of 46 carbons of goniodomin A 40 carbons(c or m in Fig. 1) were labeled by sodium acetate. Only one carbon(M in Fig. 1) was also labeled by methionine. Two carbons(A and B in Fig. 1) labeled by glucose slightly showed enhancement of signal intensities in ^<13>C NMR. The labeling patterns(Fig. 1) suggested unusual biosynthetic pathway different from polyketides pathway.

通过摄食实验研究了沟鞭藻Alexandiumhiranoi产生的聚醚大环内酯goniodomin A的生物合成。通过添加[1-^<13>C_1]、[2-^<13>C_1]或[1，2-^<13>C_2]-乙酸钠、甲基- ^<13>C-甲硫氨酸和[1-^<13>C_1]-葡萄糖对Alexandiumhiranoi培养物进行饲养实验。通过<13>对1D和2D NMR谱的分析，对goniodomin A的13 C NMR信号进行了归属。在goniodomin A的46个碳中，40个碳（图1中的c或m）被乙酸钠标记。仅一个碳（图1中的M）也被甲硫氨酸标记。葡萄糖标记的两个碳（图1中的A和B）在13 C NMR中略微显示出信号强度的增强<13>。标记模式（图1）表明不寻常的生物合成途径不同于聚酮途径。