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Study of heat satability mechanism of 5'-AMPdeaminase in dried Iwa-nori

Study of heat satability mechanism of 5'-AMPdeaminase in dried Iwa-nori
干岩海苔5-AMP脱氨酶热稳定性机制研究
批准号：
20580224
负责人：
MURAKAMI Masahiro
金额：
$3.0万
依托单位：
Kyoritsu Women's University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2008
资助国家：
日本
项目状态：
已结题
起止时间：
2008 至 2010
来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20580224/
关键词：
岩海苔5'-AMPデアミナーゼ酵素5'-IMP5'-AMPうま味安定性タンパク質AMPデアミナーゼ酵素5'-IMPAMP デアミナーゼ5' -IMP5' -AMP

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中文摘要
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英文摘要
DriedIwa-nori is one of the traditional foods and contains 5'-inosinic acid produced by 5'-AMPdeaminase. This enzyme in the DriedIwa-nori is stable by heat. In this study, contents of 5'-inosinic acid and free amino acids were measured. Activity of this enzyme was lost by heat under aqueous condition and Ca or Mg was necessary to express and stabilize this enzyme. Isolation and purification of this enzyme were tried by chromatography.
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