Utilization of antialgal compounds produced by lyticbacteria of cyanobacteria

以消除水华为目标，完成了有效抗藻类化合物的筛选。首先，用纸片法对生长在营养基质上的细菌的甲醇提取物进行了抑藻实验。第二种方法是对藻类草坪上具有菌斑形成活性的杀藻细菌进行筛选。经筛选，共获得30株杀藻细菌，通过菌落聚合酶链式反应扩增出约1.5kb的片段。用DNA测序仪对该片段进行全序列或部分序列分析。通过系统发育分析，将30株假单胞菌分为假单胞菌、芽孢杆菌、拟杆菌、节杆菌和链霉菌。分离得到抗藻类化合物K44-1-Harmane(1-甲基-β-Caroline)。通过对核磁共振谱和高分辨原子吸收光谱数据的分析，确定了该化合物的结构。Harmane和正品去甲肾上腺素(β-Caroline)对几种蓝藻菌株均有一定的抑藻活性。分离得到抗藻类化合物S4、YM-28160和渗透素A。通过核磁共振和高分辨原子吸收光谱数据的分析，确定了YM-28160和渗透素A的结构。用马菲方法和OPA-衍生物分析测定了YM-28160和渗透素A的氨基酸组成。YM-28160和渗透素A对几株蓝藻具有一定的抑藻活性。

With the aim of eliminating water bloom, the screenings for the effective antialgal compounds were accomplished. The isolation and identification of the antialgal compounds from the algicidal bacteria was described.Firstly, the MeOH extracts of bacteria which grew on the nutrient media were subjected to the antialgal assay using the paper disk method. The second method is the screening of the algicidal bacteria with plaque-forming activity on algal lawn. As the result of the screenings, 30 strains of algicidal bacteria were obtained.About 1.5 kb fragment of each bacterium was amplified by the colony PCR method. Total or partial sequence of the fragment was analyzed by the DNA sequencer. Total 30 strains were classified in several groups including Pseudomonas, Bacillus, Paenibacillus, Arthrobacter, and Streptomyces based on phylogenetic analysis.From the Pesudomonas sp. K44-1harmane (1-methy1-β-carboline) was isolated as the antialgal compound. The structure elucidation of harmane was accomplished by analyzes of NMR and HRFABMS spectra data. Harmane and authentic norharmane (β-carboline) showed the antialgal activity against several cyanobacterial strains.From Paenibacillus sp. S4, YM-28160 and permetin A were isolated as the antialgal compound. The structure elucidation of YM-28160 and permetin A was accomplished by analyzes of NMR and HRFABMS spectra data. The stereochemistries of amino acid moieties of YM-28160 and permetin A were determined by Marfey's method and analyzes of OPA-derivatives. YM-28160 and permetin A showed the antialgal activity against several cyanobacterial strains.