Utilization of antialgal compounds produced by lyticbacteria of cyanobacteria

蓝藻溶菌菌产生的抗藻化合物的利用

• 批准号: 12460090
• 负责人: MURAKAMI Masahiro
• 金额: $ 2.62万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12460090/
• 关键词: Water bloom; Lytic bacteria; Antialgal compounds; Cyanobacteria; Eutrophication; Microcystis; Anabaena; Oscillatoria; 溶藻細胞; Pseudomonas; β-carbolin; microginin; ペプチド

With the aim of eliminating water bloom, the screenings for the effective antialgal compounds were accomplished. The isolation and identification of the antialgal compounds from the algicidal bacteria was described.Firstly, the MeOH extracts of bacteria which grew on the nutrient media were subjected to the antialgal assay using the paper disk method. The second method is the screening of the algicidal bacteria with plaque-forming activity on algal lawn. As the result of the screenings, 30 strains of algicidal bacteria were obtained.About 1.5 kb fragment of each bacterium was amplified by the colony PCR method. Total or partial sequence of the fragment was analyzed by the DNA sequencer. Total 30 strains were classified in several groups including Pseudomonas, Bacillus, Paenibacillus, Arthrobacter, and Streptomyces based on phylogenetic analysis.From the Pesudomonas sp. K44-1harmane (1-methy1-β-carboline) was isolated as the antialgal compound. The structure elucidation of harmane was accomplished by analyzes of NMR and HRFABMS spectra data. Harmane and authentic norharmane (β-carboline) showed the antialgal activity against several cyanobacterial strains.From Paenibacillus sp. S4, YM-28160 and permetin A were isolated as the antialgal compound. The structure elucidation of YM-28160 and permetin A was accomplished by analyzes of NMR and HRFABMS spectra data. The stereochemistries of amino acid moieties of YM-28160 and permetin A were determined by Marfey's method and analyzes of OPA-derivatives. YM-28160 and permetin A showed the antialgal activity against several cyanobacterial strains.

以消除水华为目的，完成了有效抗藻化合物的筛选。介绍了从杀藻菌中分离和鉴定抗藻化合物的方法。首先，采用纸片法对生长在营养培养基上的细菌的MeOH提取物进行抑藻试验。第二种方法是筛选藻草坪上具有斑块形成活性的杀藻细菌。经筛选，共获得30株杀藻菌。集落PCR法扩增出每个细菌约1.5 kb的片段。用DNA测序仪分析该片段的全部或部分序列。根据系统发育分析，共30株菌株可分为假单胞菌、芽孢杆菌、Paenibacillus、节杆菌和链霉菌等。从假单胞菌sp.中分离到抗藻化合物K44-1harmane （1- methyl -β-carboline）。通过NMR和HRFABMS光谱数据分析，完成了哈曼的结构解析。哈曼和正品诺哈曼（β-碳碱）对几种蓝藻菌株显示出抗藻活性。从Paenibacillus sp. S4中分离到抗藻化合物YM-28160和渗透素A。通过NMR和HRFABMS数据分析，完成了YM-28160和渗透素A的结构解析。采用Marfey法和opa衍生物分析，确定了YM-28160和渗透素A的氨基酸部分的立体化学结构。YM-28160和渗透素A对几种蓝藻菌株均有抑藻活性。

项目成果

Shinya Kodani 他3名: "Isolation and identification of the antialgal compound from algicidal bacteria Pseudomonas sp. K-441"J. Applied Phycology. (印刷中).

Shinya Kodani 和其他 3 人：“从杀藻细菌 Pseudomonas sp. K-441 中分离和鉴定抗藻化合物”J. 应用藻类学（正在出版）。

Keishi Ishida 他4名: "Microginins, Zinc Metalloproteases Inhibitors from the Cyanobacterium Microcystis aeruginosa"Tetrahedron. 56. 8643-8656 (2000)

Keishi Ishida 和其他 4 人：“Microginins，来自铜绿微囊藻的锌金属蛋白酶抑制剂”Tetrahedron 56. 8643-8656 (2000)

Shinya Kodani et al.: "Isolation and identification of the antialga compound from Pseudomonas sp. K-441"J. Applied. Phycology. (in press).

Shinya Kodani 等人：“Pseudomonas sp. K-441 中抗藻化合物的分离和鉴定”J。

Keishi Ishida et al.: "Microginins, Zinc Metalloproteases Inhibitors from the Cyanobacterium Microcystis aeruginosa"Tetrahedron. 56. 8643-8656 (2000)

Keishi Ishida 等人：“来自蓝藻铜绿微囊藻的微基菌素、锌金属蛋白酶抑制剂”四面体。

Shinya Kodani他3名: "Isolation and identification of the antialgal compound from algicidal bacteria Pseudomonas sp. K-441"J.Applied Phycology. (印刷中).

Shinya Kodani 和其他 3 人：“从杀藻细菌 Pseudomonas sp. K-441 中分离和鉴定抗藻化合物”J.Applied Phycology（出版中）。