Utilization of antialgal compounds produced by lyticbacteria of cyanobacteria

以消除水华为目标，进行了有效抑藻化合物的筛选。本文报道了溶藻细菌中抑藻活性物质的分离和鉴定。首先，采用纸片法对生长在营养液上的溶藻细菌的甲醇提取物进行抑藻活性测定。第二种方法是在藻苔上筛选具有噬菌斑形成活性的溶藻细菌。筛选得到30株溶藻细菌，用殖民地PCR方法扩增出每株溶藻细菌约1.5kb的片段。通过DNA测序仪分析片段的全部或部分序列。通过系统发育分析，将30株菌株分为假单胞菌属（Pseudomonas）、芽孢杆菌属（Bacillus）、类芽孢杆菌属（Paenibacillus）、节杆菌属（Arthrobacter）和链霉菌属（Streptomyces），并从其中分离到一种抑藻活性化合物K44-1harmane（1-methyl-β-carboline）。通过NMR和HRFABMS数据分析，确定了哈尔烷的结构。从类芽孢杆菌（Paenibacillus sp.S4）中分离到抗藻化合物YM-28160和透皮素A（permetin A）。通过NMR和HRFABMS数据分析，确定了YM-28160和permetin A的结构。用Marvelt法和OPA衍生物分析法测定了YM-28160和permetin A的氨基酸部分的立体化学。YM-28160和permetin A对几种蓝藻菌株表现出抑藻活性。

With the aim of eliminating water bloom, the screenings for the effective antialgal compounds were accomplished. The isolation and identification of the antialgal compounds from the algicidal bacteria was described.Firstly, the MeOH extracts of bacteria which grew on the nutrient media were subjected to the antialgal assay using the paper disk method. The second method is the screening of the algicidal bacteria with plaque-forming activity on algal lawn. As the result of the screenings, 30 strains of algicidal bacteria were obtained.About 1.5 kb fragment of each bacterium was amplified by the colony PCR method. Total or partial sequence of the fragment was analyzed by the DNA sequencer. Total 30 strains were classified in several groups including Pseudomonas, Bacillus, Paenibacillus, Arthrobacter, and Streptomyces based on phylogenetic analysis.From the Pesudomonas sp. K44-1harmane (1-methy1-β-carboline) was isolated as the antialgal compound. The structure elucidation of harmane was accomplished by analyzes of NMR and HRFABMS spectra data. Harmane and authentic norharmane (β-carboline) showed the antialgal activity against several cyanobacterial strains.From Paenibacillus sp. S4, YM-28160 and permetin A were isolated as the antialgal compound. The structure elucidation of YM-28160 and permetin A was accomplished by analyzes of NMR and HRFABMS spectra data. The stereochemistries of amino acid moieties of YM-28160 and permetin A were determined by Marfey's method and analyzes of OPA-derivatives. YM-28160 and permetin A showed the antialgal activity against several cyanobacterial strains.