ANALYSIS OF THE LYTIC MECHANISM OF FILLAMENTOUS CYANOBACTERIA BY THE LITIC BACTERIA

细菌裂解丝状蓝藻的机理分析

• 批准号: 10460092
• 负责人: MURAKAMI Masahiro
• 金额: $ 2.24万
• 依托单位: THE UNIVERSITY OF TOKYO
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10460092/
• 关键词: WATER-BLOOM; LYTIC BACTERIA; PROTEASE; PROTEASE INHIBITOR; CYANOBACTERIA; PEPTIDE

A total of 212 heterotrophic bacterial strains were isolated from the colonies of the cyanobacteria Miocrocystis spp. collected at the Benkei Pond, Kyoto, Japan. These bacterial strains were assessed the productivity of extrace11ular protease. Although extrace11ular protease-active bacteria occupied around 50% of heterotrophic bacteria before the occurrence of the bloom of Microcytis (June) and the degradation period of the bloom (October), the proportion of them were lower, Iess then 20%, during the bloom of Microcystis (July, August and September). These results suggest the possibility that some protease inhibitors produced by Microcystis spp. actively growing in summer may inhibit the growth of extrace11ular protease-active bacteria living in the Microcystis colonies. Furthermore, sulfactin was isolated from the Bacillus SP., which was collected in the lake Kasumigaura at July, according to the antialgal activity.

从日本京都本庆池塘采集的微囊蓝藻菌落中分离出212株异养细菌。对这些菌株的胞外蛋白酶产率进行了评估。在微囊藻华发生前（6月）和华降解期（10月），胞外蛋白酶活性菌占异养菌总数的50%左右，而在微囊藻华发生期间（7、8、9月），胞外蛋白酶活性菌所占比例较低，不到20%。这些结果表明，在夏季活跃生长的微囊藻产生的蛋白酶抑制剂可能会抑制微囊藻菌落中胞外蛋白酶活性菌的生长。此外，根据抑藻活性，从7月采集的Kasumigaura湖芽孢杆菌中分离到磺胺素。

项目成果

Y. Itou, Hee Jae Shin, K. Ishida, and M. Murakami: "Oscillapeptins A to F, serine protease inhibitors from the three strains of Oscillatoria agardhii"Tetrahedron. 55. 6871-6882 (1999)

Y. Itou、Hee Jae Shin、K. Ishida 和 M. Murakami：“Oscillapeptins A 至 F，来自 Oscillatoria agardhii 三种菌株的丝氨酸蛋白酶抑制剂”四面体。

Shinya Kodani: "Occurrence and identification of UDP-N-acetylmuramyl-pentapeptide from the cyanobacterium Anabaena cylindrica"FEMS Microb. Lett.. 176. 321-325 (1999)

Shinya Kodani：“来自蓝藻鱼腥藻的 UDP-N-乙酰胞壁酰五肽的出现和鉴定”FEMS Microb。

S. Kodani, S. Suzuki, K. Ishida, and M. Murakami: "Five new cyanobacterial peptides from water bloom materials of Lake Teganuma"FEMS Microb. Lett. 178. 343-348 (1999)

S. Kodani、S. Suzuki、K. Ishida 和 M. Murakami：“来自手贺沼湖水华物质的五种新蓝藻肽”FEMS Microb。

Yusai Itou: "Anabaenopeptins G and H, potent carboxypeptidase A inhibitors from the cyanobacterium Oscillatoria agardhii"Bioorg. Med. Chem. Lett.. 9. 1243-1246 (1999)

Yusai Itou：“Anabaenopeptins G 和 H，来自蓝藻 Oscillatoria agardhii 的有效羧肽酶 A 抑制剂”Bioorg。

Y. Itou, S. Suzuki, K. Ishida, and M. Murakami: "Anabaenopeptins G and H, potent carboxypeptidase A inhibitors from the cyanobacterium Oscillatoria agardhii, Bioorg."Med. Chem. Lett.. 9. 1243-1246 (1999)

Y. Itou、S. Suzuki、K. Ishida 和 M. Murakami：“Anabaenopeptins G 和 H，来自蓝藻 Oscillatoria agardhii 的有效羧肽酶 A 抑制剂，Bioorg。”Med。