Molecular mechanisms of pain signal transmission and evaluation of analgesias inn vitro
疼痛信号传递的分子机制及体外镇痛评价
基本信息
- 批准号:14360171
- 负责人:
- 金额:$ 10.94万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2002
- 资助国家:日本
- 起止时间:2002 至 2004
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1)Serotonin (5-HT) potentiated Ca^<2+> response to capsaicin in cultured rat dorsal root ganglion (DRG) cells expressing 5-HT_<2A> and 5-HT_7 receptors. The increased response was inhibited by the blocker of protein kinase C or protein kinaseA. 2)In isolated neonatal rat spinal cord, electrical stimulation of the L3 ventral root elicited a fast monosynaptic reflex potential (MSR) followed by very slow potential change (sVRP) in the ipsilateral L3 ventral root. The sVRP was inhibited by α2 adrenoceptor agonists, dexmedetomidine (DEX), xylazine (XY) and clonidine (CLO) or opiate receptor agonists, morphine (MOR) and DPDPE. The order of potency was DEX>DPDPE=MOR>CLO>XY. 3)MSR and sVRP were inhibited by perfusion of the isolated spinal cord with bicarbonate-buffeted physiological solution bubbled with 20% CO_2 (pH6.7). The inhibitory effect of hypercapnia was attenuated by adenosine A1 receptor antagonists. An adenosine kinase inhibitor evoked a similar inhibitory effect on MSR and sVRP. 4)Zinc selectively potentiated sVRP, which inhibited by glutamate NMDA receptor antagonists, ketamine and AP-5. 5)DEC, XY and MOR inhibited struggling body movement induced by formalin. DEC and MOR inhibited capsaicin-induced body movement but not XY, indicative of the difference in the site of action of XY. 6)We cloned cDNA coding porcine vanilloid receptor protein (2484 bases, porcine TRPV1), which was 84% homology to rat or human TRPV1. 7)TRPV1 were expressed in HEK293 cells. In these cells, capsaicin caused increases in intracellular Ca^<2+> concentration and non-selective cation currents. Porcine TRPV1 was activated by reducing extracellular pH or increasing temperature of the perfusing solution (>42℃), indicative of fiunctionin Ca2+g polymodal receptors. 8)Although responsiveness to vanilloid agonists in porcine TRPV1 was quite similar to that in rat TRPV1, the reduction of extracellular pH was more effective in porcine than rat TRPV1.
1)5-羟色胺(5-HT)增强培养的大鼠背根神经节(DRG)细胞表达5-羟色胺(5-羟色胺)和5-羟色胺(5-HT7)受体的钙反应。这种增强的反应可被蛋白激酶C或蛋白激酶A阻滞剂所抑制。2)在分离的新生大鼠脊髓,电刺激L3前根引起同侧L3前根的快单突触反射电位(MSR)和极慢电位改变(SVRP)。α-2肾上腺素能受体激动剂右美托咪啶、赛拉津和可乐定或阿片受体激动剂吗啡和二甲基苯丙胺均能抑制SVRP。药效顺序为DEX>;DPDPE=MOR>;CLO>;XY。3)用含20%CO_2(pH 6.7)的碳酸氢盐冲击生理溶液灌流脊髓,可抑制MSR和sVRP。腺苷A1受体拮抗剂可减弱高碳酸血症的抑制作用。腺苷激酶抑制剂对MSR和SVRP也有类似的抑制作用。4)锌能选择性增强谷氨酸NMDA受体拮抗剂氯胺酮和AP-5抑制的sVRP。5)DEC、XY和MOR可抑制福尔马林诱导的身体挣扎运动。DEC和MOR抑制辣椒素诱导的身体运动,但不抑制XY,这表明XY的作用部位不同。6)克隆了猪香草素受体蛋白编码基因(2484个碱基,猪TRPV1),与大鼠和人TRPV1有84%的同源性。7)TRPV1在HEK293细胞中表达。在这些细胞中,辣椒素引起细胞内钙离子浓度和非选择性阳离子电流增加。降低细胞外pH或升高灌流液温度(>;42℃)可激活猪TRPV1,表明其具有钙离子多模式受体的功能。8)猪TRPV1对香草素类激动剂的反应性与大鼠TRPV1相似,但降低细胞外pH的效果优于大鼠TRPV1。
项目成果
期刊论文数量(46)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kazuki Yonekubo: "Inhibitory effects of cortical steroids and adrenocorticotropic hormone on catecholamine secretion in guinea-pig perfused adrenal glands"Autonomic & Autocoid Pharmacology. 22. 93-101 (2002)
Kazuki Yonekubo:“皮质类固醇和促肾上腺皮质激素对豚鼠肾上腺灌注儿茶酚胺分泌的抑制作用”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Toshio Ohta: "Ca2+ store-independent augmentation of [Ca2+]i responses to G-protein coupled Receptor activation in recombinatly TRPC5-expressed rat pheochromocytoma (PC12) cells."Neuroscience Letters. (In Press). (2004)
Toshio Ohta:“在重组 TRPC5 表达的大鼠嗜铬细胞瘤 (PC12) 细胞中,[Ca2]i 对 G 蛋白偶联受体激活的响应与 Ca2 储存无关的增强。”《神经科学快报》。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Ca2+ store-independent augmentation of [Ca2+]i responses to G-protein coupled receptor activation in recombinantly TRPC5-expressed rat pheochromocytoma (PC12) cells
- DOI:10.1016/j.neulet.2004.01.028
- 发表时间:2004-04
- 期刊:
- 影响因子:2.5
- 作者:T. Ohta;M. Morishita;Y. Mori;S. Ito
- 通讯作者:T. Ohta;M. Morishita;Y. Mori;S. Ito
Inhibitory effects of cortical steroids and adrenocorticotropic hormone on catecholoamine secretion in guinea-pig perfused adrenal glands.
皮质类固醇和促肾上腺皮质激素对豚鼠肾上腺灌注儿茶酚胺分泌的抑制作用。
- DOI:
- 发表时间:2002
- 期刊:
- 影响因子:0
- 作者:Kazuki Yonekubo;Toshio Ohta;Yoshikazu Nakazato;Shigeo Ito
- 通讯作者:Shigeo Ito
Two distinct inhibitory actions of steroids on cholinoceptor-mediated secretion of catecholamine from guinea-pig adrenal medullary cells
- DOI:10.1016/s0304-3940(02)01313-7
- 发表时间:2003-02
- 期刊:
- 影响因子:2.5
- 作者:K. Yonekubo;T. Ohta;S. Ito
- 通讯作者:K. Yonekubo;T. Ohta;S. Ito
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ITO Shigeo其他文献
ITO Shigeo的其他文献
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{{ truncateString('ITO Shigeo', 18)}}的其他基金
Quantitative analysis of nociceptive and motor reflexes in genetically modified mice
转基因小鼠伤害性和运动反射的定量分析
- 批准号:
23380170 - 财政年份:2011
- 资助金额:
$ 10.94万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Species differences in nociceptive transmissions
伤害性传播的物种差异
- 批准号:
23658231 - 财政年份:2011
- 资助金额:
$ 10.94万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
The research of neuroimaging in HIV-positive
HIV阳性患者的神经影像学研究
- 批准号:
22791145 - 财政年份:2010
- 资助金额:
$ 10.94万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Quantitative analysis of pain response in isolated spinal cord and cultured dorsal root ganglion cell
离体脊髓和培养背根神经节细胞疼痛反应的定量分析
- 批准号:
19380163 - 财政年份:2007
- 资助金额:
$ 10.94万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Multiple scattering analysis for estimating the structure of heavy rain using by the satellite radar
利用卫星雷达估算暴雨结构的多重散射分析
- 批准号:
19560430 - 财政年份:2007
- 资助金额:
$ 10.94万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Functional and pathphysiological study in serotonin-containing chemorecptor cells
含血清素化学感受器细胞的功能和病理生理学研究
- 批准号:
12460128 - 财政年份:2000
- 资助金额:
$ 10.94万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
The effects of the backscattering enhancement on signals of rain radar
后向散射增强对雨雷达信号的影响
- 批准号:
11650434 - 财政年份:1999
- 资助金额:
$ 10.94万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Multiple Scattering Effects of Electromagnetic Waves from Randomly Distributed Particles and Its Application to a High Precision Radar
随机分布粒子电磁波的多重散射效应及其在高精度雷达中的应用
- 批准号:
08650515 - 财政年份:1996
- 资助金额:
$ 10.94万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Intracellular signal transduction of parathyroid hormone receptor activation and its pathphysiology
甲状旁腺激素受体激活的细胞内信号转导及其病理生理学
- 批准号:
07456129 - 财政年份:1995
- 资助金额:
$ 10.94万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Isolation and cloning of VIP receptors.
VIP 受体的分离和克隆。
- 批准号:
03454101 - 财政年份:1991
- 资助金额:
$ 10.94万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)