Functional and pathphysiological study in serotonin-containing chemorecptor cells
含血清素化学感受器细胞的功能和病理生理学研究
基本信息
- 批准号:12460128
- 负责人:
- 金额:$ 9.09万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2000
- 资助国家:日本
- 起止时间:2000 至 2001
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Serotonin (5-HT) -containing chemoreceptor cells were characterized using a whole-cell patch clamp technique. (1) 5-HT-containing chemoreceptor cells isolated from chicken thoracic aorta have voltage-gated Na^+ channels, L- and N-type Ca^<2+> channels and K^+ channels including oxygen-sensitive K^+ channels. (2) α-Bungarotoxin-sensitive and insensitive nicotinic ACh receptors were present in these cells. (3) Although we tried to isolate epitherial cells containing 5-HT (neutral red-positive cells) from the guinea-pig and rat intestinal mucosae, we could not obtain the cells responding to voltage-pulses under the voltage-clamp condition. (4) Excess KCl and veratridine evoked 5-HT output from the chicken thoracic aorta, which was inhibited by Ca^<2+> channel antagonists. ACh, nicotine and DMPP also evoked 5-HT output partly inhibited by α-bungarotoxin. (5) p-Chloroamphetamine (PCA) evoked time- and concentration-dependent 5-HT output from the aortic tissues. 5-HT reuptake inhibitors, flu … More voxamine, fluoxetine and clomipramine(CLO) caused a dose-dependent increase in 5-HT in the incubation medium, which were insensitive to extracellular Ca^<2+> removal. (6) CLO and PCA caused a temperature-dependent 5-HT output. CLO-induced 5-HT output was inhibited by extracellular Na^+ removal but not PCA-induced one. (7) 5-HT evoked oscillatory changes in tension and intracellular Ca^<2+> concentration ([Ca^<2+>]i) in the rat basilar artery. In vascular smooth muscles cells isolated from the rat basilar artery, UTP elicited increases in [Ca^<2+>]i in all cells examined, but 5-HT hardly evoked the response. (8) In PC12 cells, a vesicular amine uptake inhibitor, reserpine, decreased the content of dopamine but not ATP in the secretory vesicles. A vesicular H^+-pump ATPase inhibitor, bafilomycine A1, decreased both contents. (9) In adrenal chromaffin cells, both muscarine and caffeine elicited Ca^<2+>-activated K^+ currents concomitant with increases in [Ca^<2+>]i, but caffeine was more effective in eleciting K^+ currents even when both drugs evoked the increase of [Ca^<2+>]i to the same extent. Opioid agonists inhibited voltage-gated Ca^<2+> channels through G proteins. Less
使用全细胞膜片钳技术对含有血清素 (5-HT) 的化学感受器细胞进行表征。 (1)从鸡胸主动脉分离的含5-HT的化学感受器细胞具有电压门控Na^+通道、L型和N型Ca^2+通道以及K^+通道,包括氧敏感的K^+通道。 (2)这些细胞中存在对α-金环蛇毒素敏感和不敏感的烟碱ACh受体。 (3)虽然我们尝试从豚鼠和大鼠肠粘膜中分离出含有5-HT(中性红阳性细胞)的上皮细胞,但我们无法获得在电压钳条件下对电压脉冲做出反应的细胞。 (4)过量的KCl和藜芦定引起鸡胸主动脉5-HT输出,该输出被Ca 2+ 通道拮抗剂抑制。 ACh、尼古丁和 DMPP 也会引起 5-HT 输出,而 α-银环蛇毒素部分抑制该输出。 (5) 对氯苯丙胺 (PCA) 引起主动脉组织的时间和浓度依赖性 5-HT 输出。 5-HT再摄取抑制剂、流感……更多伏沙明、氟西汀和氯米帕明(CLO)引起培养介质中5-HT的剂量依赖性增加,其对细胞外Ca^2+去除不敏感。 (6) CLO 和 PCA 引起温度依赖性 5-HT 输出。 CLO 诱导的 5-HT 输出受到细胞外 Na^+ 去除的抑制,但 PCA 诱导的 5-HT 输出不受抑制。 (7)5-HT引起大鼠基底动脉张力和细胞内Ca 2+ 浓度([Ca 2+ ]i)的振荡变化。在从大鼠基底动脉分离的血管平滑肌细胞中,UTP引起所有检查细胞中[Ca 2+ ]i的增加,但5-HT几乎不引起反应。 (8)在PC12细胞中,囊泡胺摄取抑制剂利血平降低了分泌囊泡中多巴胺的含量,但不降低ATP的含量。囊泡 H^+ 泵 ATP 酶抑制剂巴弗洛霉素 A1 可降低这两种含量。 (9)在肾上腺嗜铬细胞中,毒蕈碱和咖啡因均可引起Ca^<2+>激活的K^+电流,并伴随[Ca^<2+>]i的增加,但即使两种药物引起[Ca^<2+>]i的增加程度相同,咖啡因在引起K^+电流方面更有效。阿片类激动剂通过G蛋白抑制电压门控Ca 2+ 通道。较少的
项目成果
期刊论文数量(36)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Toshio Ohta: "Ca^<2+>-dependent K^+ current and exocytosis in response to caffeine and muscarine in voltage-clamped guinea-pig adrenal chromaffin cells"Journal of Neurochemistry. 78. 1243-1255 (2001)
Toshio Ohta:“电压钳位豚鼠肾上腺嗜铬细胞中响应咖啡因和毒蕈碱的Ca^2依赖性K^电流和胞吐作用”神经化学杂志。
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- 影响因子:0
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Ito S., Ohta T., Kasai Y., Yonekubo K., Nakazato Y.: "Heterogeneity of neuronal nicotinic acetylcholine receptors in 5-HT-containing chemoreceptor cells of the chicken aorta"British Journal of Pharmacology. 132. 1934-1940 (2001)
Ito S.、Ohta T.、Kasai Y.、Yonekubo K.、Nakazato Y.:“鸡主动脉含 5-HT 化学感受器细胞中神经元烟碱乙酰胆碱受体的异质性”英国药理学杂志。
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- 影响因子:0
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Shigeo lto: "Heterogeneity of neuronal nicotinic acetylchollne receptors in 5-HT-containing chemoreceptor cells of the chicken aorta"British Journal of Pharmacology. 132. 1934-1940 (2001)
Shigeo lto:“鸡主动脉含 5-HT 化学感受器细胞中神经元烟碱乙酰胆碱受体的异质性”英国药理学杂志。
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- 影响因子:0
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Kasai Y, Ohta T., Nakazato Y., Ito S.: "Release of dopamine and ATP from PC12 cells treated with dexamethasone, reserpine and bafilomycin A1"Journal of Veterinary Medical Science. 63(4). 367-372 (2001)
Kasai Y、Ohta T.、Nakazato Y.、Ito S.:“用地塞米松、利血平和巴弗洛霉素 A1 处理的 PC12 细胞释放多巴胺和 ATP”《兽医医学科学杂志》。
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- 影响因子:0
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Eriko Karoei: "Vasopressin-induced contraction in the rat basilar artery in vitro"European Journal of Pharmacology. (In press). (2001)
Eriko Karoei:“体外加压素诱导大鼠基底动脉收缩”欧洲药理学杂志。
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ITO Shigeo其他文献
ITO Shigeo的其他文献
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{{ truncateString('ITO Shigeo', 18)}}的其他基金
Quantitative analysis of nociceptive and motor reflexes in genetically modified mice
转基因小鼠伤害性和运动反射的定量分析
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23380170 - 财政年份:2011
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Species differences in nociceptive transmissions
伤害性传播的物种差异
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HIV阳性患者的神经影像学研究
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19560430 - 财政年份:2007
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Molecular mechanisms of pain signal transmission and evaluation of analgesias inn vitro
疼痛信号传递的分子机制及体外镇痛评价
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14360171 - 财政年份:2002
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$ 9.09万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
The effects of the backscattering enhancement on signals of rain radar
后向散射增强对雨雷达信号的影响
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11650434 - 财政年份:1999
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$ 9.09万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Multiple Scattering Effects of Electromagnetic Waves from Randomly Distributed Particles and Its Application to a High Precision Radar
随机分布粒子电磁波的多重散射效应及其在高精度雷达中的应用
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08650515 - 财政年份:1996
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Intracellular signal transduction of parathyroid hormone receptor activation and its pathphysiology
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07456129 - 财政年份:1995
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VIP 受体的分离和克隆。
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$ 9.09万 - 项目类别:
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