Functional and pathphysiological study in serotonin-containing chemorecptor cells

含血清素化学感受器细胞的功能和病理生理学研究

基本信息

  • 批准号:
    12460128
  • 负责人:
  • 金额:
    $ 9.09万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    2000
  • 资助国家:
    日本
  • 起止时间:
    2000 至 2001
  • 项目状态:
    已结题

项目摘要

Serotonin (5-HT) -containing chemoreceptor cells were characterized using a whole-cell patch clamp technique. (1) 5-HT-containing chemoreceptor cells isolated from chicken thoracic aorta have voltage-gated Na^+ channels, L- and N-type Ca^<2+> channels and K^+ channels including oxygen-sensitive K^+ channels. (2) α-Bungarotoxin-sensitive and insensitive nicotinic ACh receptors were present in these cells. (3) Although we tried to isolate epitherial cells containing 5-HT (neutral red-positive cells) from the guinea-pig and rat intestinal mucosae, we could not obtain the cells responding to voltage-pulses under the voltage-clamp condition. (4) Excess KCl and veratridine evoked 5-HT output from the chicken thoracic aorta, which was inhibited by Ca^<2+> channel antagonists. ACh, nicotine and DMPP also evoked 5-HT output partly inhibited by α-bungarotoxin. (5) p-Chloroamphetamine (PCA) evoked time- and concentration-dependent 5-HT output from the aortic tissues. 5-HT reuptake inhibitors, flu … More voxamine, fluoxetine and clomipramine(CLO) caused a dose-dependent increase in 5-HT in the incubation medium, which were insensitive to extracellular Ca^<2+> removal. (6) CLO and PCA caused a temperature-dependent 5-HT output. CLO-induced 5-HT output was inhibited by extracellular Na^+ removal but not PCA-induced one. (7) 5-HT evoked oscillatory changes in tension and intracellular Ca^<2+> concentration ([Ca^<2+>]i) in the rat basilar artery. In vascular smooth muscles cells isolated from the rat basilar artery, UTP elicited increases in [Ca^<2+>]i in all cells examined, but 5-HT hardly evoked the response. (8) In PC12 cells, a vesicular amine uptake inhibitor, reserpine, decreased the content of dopamine but not ATP in the secretory vesicles. A vesicular H^+-pump ATPase inhibitor, bafilomycine A1, decreased both contents. (9) In adrenal chromaffin cells, both muscarine and caffeine elicited Ca^<2+>-activated K^+ currents concomitant with increases in [Ca^<2+>]i, but caffeine was more effective in eleciting K^+ currents even when both drugs evoked the increase of [Ca^<2+>]i to the same extent. Opioid agonists inhibited voltage-gated Ca^<2+> channels through G proteins. Less
使用全细胞膜片钳技术对含有血清素 (5-HT) 的化学感受器细胞进行表征。 (1)从鸡胸主动脉分离的含5-HT的化学感受器细胞具有电压门控Na^+通道、L型和N型Ca^2+通道以及K^+通道,包括氧敏感的K^+通道。 (2)这些细胞中存在对α-金环蛇毒素敏感和不敏感的烟碱ACh受体。 (3)虽然我们尝试从豚鼠和大鼠肠粘膜中分离出含有5-HT(中性红阳性细胞)的上皮细胞,但我们无法获得在电压钳条件下对电压脉冲做出反应的细胞。 (4)过量的KCl和藜芦定引起鸡胸主动脉5-HT输出,该输出被Ca 2+ 通道拮抗剂抑制。 ACh、尼古丁和 DMPP 也会引起 5-HT 输出,而 α-银环蛇毒素部分抑制该输出。 (5) 对氯苯丙胺 (PCA) 引起主动脉组织的时间和浓度依赖性 5-HT 输出。 5-HT再摄取抑制剂、流感……更多伏沙明、氟西汀和氯米帕明(CLO)引起培养介质中5-HT的剂量依赖性增加,其对细胞外Ca^2+去除不敏感。 (6) CLO 和 PCA 引起温度依赖性 5-HT 输出。 CLO 诱导的 5-HT 输出受到细胞外 Na^+ 去除的抑制,但 PCA 诱导的 5-HT 输出不受抑制。 (7)5-HT引起大鼠基底动脉张力和细胞内Ca 2+ 浓度([Ca 2+ ]i)的振荡变化。在从大鼠基底动脉分离的血管平滑肌细胞中,UTP引起所有检查细胞中[Ca 2+ ]i的增加,但5-HT几乎不引起反应。 (8)在PC12细胞中,囊泡胺摄取抑制剂利血平降低了分泌囊泡中多巴胺的含量,但不降低ATP的含量。囊泡 H^+ 泵 ATP 酶抑制剂巴弗洛霉素 A1 可降低这两种含量。 (9)在肾上腺嗜铬细胞中,毒蕈碱和咖啡因均可引起Ca^<2+>激活的K^+电流,并伴随[Ca^<2+>]i的增加,但即使两种药物引起[Ca^<2+>]i的增加程度相同,咖啡因在引起K^+电流方面更有效。阿片类激动剂通过G蛋白抑制电压门控Ca 2+ 通道。较少的

项目成果

期刊论文数量(36)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Toshio Ohta: "Ca^<2+>-dependent K^+ current and exocytosis in response to caffeine and muscarine in voltage-clamped guinea-pig adrenal chromaffin cells"Journal of Neurochemistry. 78. 1243-1255 (2001)
Toshio Ohta:“电压钳位豚鼠肾上腺嗜铬细胞中响应咖啡因和毒蕈碱的Ca^2依赖性K^电流和胞吐作用”神经化学杂志。
  • DOI:
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  • 期刊:
  • 影响因子:
    0
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  • 通讯作者:
Ito S., Ohta T., Kasai Y., Yonekubo K., Nakazato Y.: "Heterogeneity of neuronal nicotinic acetylcholine receptors in 5-HT-containing chemoreceptor cells of the chicken aorta"British Journal of Pharmacology. 132. 1934-1940 (2001)
Ito S.、Ohta T.、Kasai Y.、Yonekubo K.、Nakazato Y.:“鸡主动脉含 5-HT 化学感受器细胞中神经元烟碱乙酰胆碱受体的异质性”英国药理学杂志。
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  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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Shigeo lto: "Heterogeneity of neuronal nicotinic acetylchollne receptors in 5-HT-containing chemoreceptor cells of the chicken aorta"British Journal of Pharmacology. 132. 1934-1940 (2001)
Shigeo lto:“鸡主动脉含 5-HT 化学感受器细胞中神经元烟碱乙酰胆碱受体的异质性”英国药理学杂志。
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  • 影响因子:
    0
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  • 通讯作者:
Kasai Y, Ohta T., Nakazato Y., Ito S.: "Release of dopamine and ATP from PC12 cells treated with dexamethasone, reserpine and bafilomycin A1"Journal of Veterinary Medical Science. 63(4). 367-372 (2001)
Kasai Y、Ohta T.、Nakazato Y.、Ito S.:“用地塞米松、利血平和巴弗洛霉素 A1 处理的 PC12 细胞释放多巴胺和 ATP”《兽医医学科学杂志》。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Eriko Karoei: "Vasopressin-induced contraction in the rat basilar artery in vitro"European Journal of Pharmacology. (In press). (2001)
Eriko Karoei:“体外加压素诱导大鼠基底动脉收缩”欧洲药理学杂志。
  • DOI:
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  • 影响因子:
    0
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ITO Shigeo其他文献

ITO Shigeo的其他文献

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{{ truncateString('ITO Shigeo', 18)}}的其他基金

Quantitative analysis of nociceptive and motor reflexes in genetically modified mice
转基因小鼠伤害性和运动反射的定量分析
  • 批准号:
    23380170
  • 财政年份:
    2011
  • 资助金额:
    $ 9.09万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Species differences in nociceptive transmissions
伤害性传播的物种差异
  • 批准号:
    23658231
  • 财政年份:
    2011
  • 资助金额:
    $ 9.09万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
The research of neuroimaging in HIV-positive
HIV阳性患者的神经影像学研究
  • 批准号:
    22791145
  • 财政年份:
    2010
  • 资助金额:
    $ 9.09万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Quantitative analysis of pain response in isolated spinal cord and cultured dorsal root ganglion cell
离体脊髓和培养背根神经节细胞疼痛反应的定量分析
  • 批准号:
    19380163
  • 财政年份:
    2007
  • 资助金额:
    $ 9.09万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Multiple scattering analysis for estimating the structure of heavy rain using by the satellite radar
利用卫星雷达估算暴雨结构的多重散射分析
  • 批准号:
    19560430
  • 财政年份:
    2007
  • 资助金额:
    $ 9.09万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Molecular mechanisms of pain signal transmission and evaluation of analgesias inn vitro
疼痛信号传递的分子机制及体外镇痛评价
  • 批准号:
    14360171
  • 财政年份:
    2002
  • 资助金额:
    $ 9.09万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
The effects of the backscattering enhancement on signals of rain radar
后向散射增强对雨雷达信号的影响
  • 批准号:
    11650434
  • 财政年份:
    1999
  • 资助金额:
    $ 9.09万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Multiple Scattering Effects of Electromagnetic Waves from Randomly Distributed Particles and Its Application to a High Precision Radar
随机分布粒子电磁波的多重散射效应及其在高精度雷达中的应用
  • 批准号:
    08650515
  • 财政年份:
    1996
  • 资助金额:
    $ 9.09万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Intracellular signal transduction of parathyroid hormone receptor activation and its pathphysiology
甲状旁腺激素受体激活的细胞内信号转导及其病理生理学
  • 批准号:
    07456129
  • 财政年份:
    1995
  • 资助金额:
    $ 9.09万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Isolation and cloning of VIP receptors.
VIP 受体的分离和克隆。
  • 批准号:
    03454101
  • 财政年份:
    1991
  • 资助金额:
    $ 9.09万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)

相似国自然基金

5-羟色胺在低雌激素致体温调节中枢异常的作用
  • 批准号:
    81070462
  • 批准年份:
    2010
  • 资助金额:
    10.0 万元
  • 项目类别:
    面上项目

相似海外基金

Sex differences in stress, neuroendocrine and serotonin (5-hydroxytryptamine) 5-HT1A receptor responses.
压力、神经内分泌和血清素(5-羟色胺)5-HT1A 受体反应的性别差异。
  • 批准号:
    304567
  • 财政年份:
    2014
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    $ 9.09万
  • 项目类别:
    Operating Grants
Sex differences in stress, neuroendocrine and serotonin (5-hydroxytryptamine) 5-HT1A receptor responses.
压力、神经内分泌和血清素(5-羟色胺)5-HT1A 受体反应的性别差异。
  • 批准号:
    299297
  • 财政年份:
    2013
  • 资助金额:
    $ 9.09万
  • 项目类别:
    Operating Grants
Mechanisms of 5-hydroxytryptamine-induced hypotension
5-羟色胺诱发低血压的机制
  • 批准号:
    8018166
  • 财政年份:
    2010
  • 资助金额:
    $ 9.09万
  • 项目类别:
Mechanisms of 5-hydroxytryptamine-induced hypotension
5-羟色胺诱发低血压的机制
  • 批准号:
    8437225
  • 财政年份:
    2010
  • 资助金额:
    $ 9.09万
  • 项目类别:
Mechanisms of 5-hydroxytryptamine-induced hypotension
5-羟色胺诱发低血压的机制
  • 批准号:
    7801860
  • 财政年份:
    2010
  • 资助金额:
    $ 9.09万
  • 项目类别:
Mechanisms of 5-hydroxytryptamine-induced hypotension
5-羟色胺诱发低血压的机制
  • 批准号:
    8209053
  • 财政年份:
    2010
  • 资助金额:
    $ 9.09万
  • 项目类别:
Pharmacological studies on responsiveness of 5-hydroxytryptamine to overcome perioperative spasm of coronary artery bypass graft in diabetes mellitus
5-羟色胺克服糖尿病冠状动脉搭桥术围手术期痉挛的药理学研究
  • 批准号:
    21790104
  • 财政年份:
    2009
  • 资助金额:
    $ 9.09万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
The nociceptive mechanism of 5-hydroxytryptamine released into the peripheral tissue in acute inflammatory pain in rats
5-羟色胺释放到外周组织对大鼠急性炎症痛的伤害机制
  • 批准号:
    19791056
  • 财政年份:
    2007
  • 资助金额:
    $ 9.09万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Potential roles of 5-hydroxytryptamine in diseases affecting the colon
5-羟色胺在结肠疾病中的潜在作用
  • 批准号:
    nhmrc : 143509
  • 财政年份:
    2001
  • 资助金额:
    $ 9.09万
  • 项目类别:
    NHMRC Project Grants
Peripheral and central involvement of 5-hydroxytryptamine in control of interdigestive migrating contractions by motilin.
5-羟色胺的外周和中枢参与胃动素控制消化间期迁移性收缩。
  • 批准号:
    04454239
  • 财政年份:
    1992
  • 资助金额:
    $ 9.09万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
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