Quantitative analysis of pain response in isolated spinal cord and cultured dorsal root ganglion cell

离体脊髓和培养背根神经节细胞疼痛反应的定量分析

• 批准号: 19380163
• 负责人: ITO Shigeo
• 金额: $ 13.06万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19380163/
• 关键词: バニロイド受容体; TRPV1チャネル; 背根神経節細胞; 新生仔ラット脊髄; ヒスタミン; 5-ヒドロキシトリプタミン; アデノシン; 腸管神経叢細胞; 新生ラット脊髄標本; リポポリサッカライド; 腸管神経細胞; 後根神経; ブラジキニン; 低酸素刺激; TPRV1チャネル; 痛覚過敏; 後根神経節; 5-hydroxytryptamine; カプサイシンン

We found that vanilloid receptor (TRPV1) channels, being key proteins for pain generation, were activated by mustard oil or methylsalicylate in addition to a vanilloid compound such as capsaicin and that the opening of the channels was controlled by extracellular Na^+ ions. It was demonstrated that histamine and 5-hydroxy- tryptamine, which were released at inflammation sites, augmented pain due to TRPV1 channel activation through respective receptors for these amines, and that adenosine release was associated with the analgesic action in response to hypercapnia.

我们发现，辣椒素受体（TRPV 1）通道是产生疼痛的关键蛋白，除了辣椒素等辣椒素化合物外，芥子油或水杨酸甲酯也能激活TRPV 1通道，而且通道的开放受细胞外Na^+离子的控制。已经证明，在炎症部位释放的组胺和5-羟色胺通过这些胺的各自受体激活TRPV 1通道而增加疼痛，并且腺苷释放与响应于高碳酸血症的镇痛作用相关。

项目成果

新生ラット脊髄反射電位に対するメタンフェタミンの作用

甲基苯丙胺对新生大鼠脊髓反射电位的影响

• 发表时间: 2009
• 作者: 川本研介;岩崎健幸;乙黒兼一;太田利男;伊藤茂男
• 通讯作者: 伊藤茂男

Novel gating and sensitizing mechanism of capsaicin receptor (TRPV1) - Tonic inhibitory regulation of extracellular sodium through the external protonation sites on TRPV1

• DOI: 10.1074/jbc.m709377200
• 发表时间: 2008-04-04
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Ohta, Toshio;Imagawa, Toshiaki;Ito, Shigeo
• 通讯作者: Ito, Shigeo

Involvement of prostaglandin E2 derived from enteric glial cells in the action of bradykinin in cultured rat myenteric neurons

• DOI: 10.1016/j.neuroscience.2006.12.052
• 发表时间: 2007-03
• 期刊: Neuroscience
• 影响因子: 3.3
• 作者: M. Murakami;T. Ohta;K. Otsuguro;S. Ito

Amelioration of amemia in the ICGN mouse, a renal anemia model, with a subcutaneous bolus injection of erythropoietin a dsorbed to hydroxyapatite matrix.

通过皮下推注注射吸附到羟基磷灰石基质的促红细胞生成素，改善 ICGN 小鼠（肾性贫血模型）的贫血症。

• 发表时间: 2009
• 期刊: Journal of Veterinary Medical Science 71(10)
• 作者: Nagasaki k;Ikoma T;Katsuda S;Tonegawa T;Tanaka J;Ohyama M;Hayashida K;Nakamura T;Sato H;Ito S;Sasaki N;Agui T
• 通讯作者: Agui T

エリスロポエチン含有ハイドロキシアパタイトを用いた腎性貧血の持続的改善

使用含促红细胞生成素的羟基磷灰石持续改善肾性贫血

• 发表时间: 2009
• 作者: 長崎健一;生駒俊之;勝田真一;利根川亨;田中順三;中村鉄平;佐藤秀隆;伊藤茂男;佐々木宣哉;安居院高志
• 通讯作者: 安居院高志