Functional analysis of fatty acid binding proteins in immune and neural tissues.

在脂肪酸结合蛋白（FABPs）中，详细研究了脑（B）-和表皮（E）-FABPs在小鼠不同器官/组织中的定位，并制备了这两种FABPs的基因敲除小鼠，分析了它们在神经免疫系统中的表型。B-FABP在出生前主要定位于脑室生发带和放射状胶质细胞，出生后主要定位于星形胶质细胞; E-FABP在出生后主要定位于神经元和胶质细胞，出生后主要定位于星形胶质细胞。而B-FABP仅定位于肝枯否细胞，E-FABP定位于脾树突状细胞，腹腔和肺泡巨噬细胞，和胸腺上皮细胞的成年小鼠。利用E-FABP作为树突状细胞特异性标志物的新优势，首次揭示了树突状细胞对脂多糖诱导的凋亡脾细胞的吞噬作用。E-FABP纯合子小鼠的基础经皮失水量低于野生型小鼠。丙酮破坏皮肤的透水屏障后，经皮失水的恢复延迟，但表皮的超微结构和脂质含量无明显差异。从E-FABP纯合子小鼠中分离的树突状细胞显示IL-12分泌显著减少。B-FABP纯合子小鼠表现出学习记忆能力下降、焦虑。

Among fatty acid binding proteins (FABPs), the localization of brain(B)-and epidermal (E)-FABPs were examined in details in varous organs/tissues of mice, and gene knockout mice for the two FABPs were generated and their phenotypes were analysed in neuro-immune systems. While B-FABP was localized in the ventricular germinal zone and radial glial cells at prenatal stages and confined to the astrocytes in postnatal brain, E-FABP was localized in neurons and glia at the perinatal stage and confined to astrocytes in postnatal brain. While B-FABP was localized only in hepatic Kupffer cells, E-FABP was localized in splenic dendritic cells, peritoneal and alveolar macrophages, and thymic epithelical cells of adult mice. With the new advantage of E-FABP as a specific marker for the dendritc cells, the phagocytosis of lipopolysaccharide-induced apoptotic splenocytes in situ by the dendritic cells was first revealed. The basal transepidermal water loss of homozygous mice for E-FABP was lower than that of the wild mice. When the water permeability barrier of skin was disrupted by aceton application, recovery in transepidermal water loss was delayed, although no marked differences ware detected in ultrastructure and lipid contents of epidermis. Dendritic cells isolated from E-FABP-homozygous mice showed a marked decrease of IL-12 secretion. B-FABP-homozygous mice showed deterioration of learnining, memory anxiety.