Molecular and Cell Biological Analysis of Lipid Kinases in Relation to Signaling and Vesicle Traffic.
Molecular and Cell Biological Analysis of Lipid Kinases in Relation to Signaling and Vesicle Traffic.
批准号:
09470001
负责人:
KONDO Hisatake
金额:
$8.32万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
1997
资助国家:
日本
项目状态:
已结题
起止时间:
1997 至 1998
中文摘要
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英文摘要
The cloning and characterization of a novel class II phosphoinositide (PI) 3-kinase was succeeded from the cDNA library of regenerating rat liver. This enzyme molecule composed of 1505 amino acids contained a C2 domain and displayed a restricted substrate specificity for PtdIns and PtdIns 4-P and, thus termed PI3K-IIgamma. This molecule was localized in the juxtanuclear Golgi region in the over-expressed in vitro cells. The mRNA expression was confined to the liver throughout the development and its expression increased during liver regeneration after partial hepatectomy.The gene expression for a subtype of phosphatidate (PA) phosphatase termed Dri42 was confined to the ventricular germinal zone of embryonic brain. PAP catalyses a dephosphorylation of PA to produce diacylglycerol (DG), an reverse process of phosphorylation by DG kinase whose molecular identification has been intensively performed by us. The localization of mRNAs for synaptojanin, inositol 5-phosphatase, in the brain was also revelaed by in situ hybridization histochemistry. Synaptojanin 1 mRNA was expressed in almost all neurons of the brain throughout developing and mature stages while synaptojanin 2 mRNA was first detected in neurons on early postnatal stages and the expression was evident in the white matters presumptive oligodendrocytes as the postnatal development proceeded. All these findings on dephosphorylation were instructive for understanding the significance of phosphorylation by lipid kinases.The gene expression localization of CDP-DG synthase and phosphatidylinositol synthase, both of which are involved in the two sequential PI-metabolic processes, was analyzed and some discrepant expression patterns were found spatio-temporally in developing and mature brain. The gene expression localization of phospholipase D in the brain is also under way by in situ hybridization histochemistry.
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Kudo, M., Saito, S., Sakagami, H.& Kondo, H.: "Localization of synaptojanin mRNAs in the rat brain." Mol.Brain.Res.64. 178-185
工藤,M.,齐藤,S.,坂上,H.
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Saito, S., Sakagami, H., Tonosaki, A.& Kondo, H.: "Localization of mRNAs for CDP-diacylglycerol synthase and phosphatidylinositol synthase in the brain and retina of developing and adult rats." Dev.Brain.Res.110 (1). 21-30
Saito, S.、Sakagami, H.、Tonosaki, A.
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One, F., Nakagawa, T., Saito, S., Owada, Y., Sakagami, H., Goto, K., Suzuki, M., Matsuo, S.& Kondo, H.: "A novel class-II phospho-inositide 3-kinase prodominantly expressed in the liver and enhanced expression during liver regeneration." J.Biol.Chem.273 (
一,F.,中川,T.,斋藤,S.,小和田,Y.,坂上,H.,后藤,K.,铃木,M.,松尾,S.
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H.Kondo: "Expression of mRNA for AKT,serine-threonine protein kinase in the brain during development and its transient enhancement following axotomy of hypglossal nerve" J.Mol.Neurosei.9(1). 27-33 (1997)
H.Kondo:“发育过程中大脑中 AKT、丝氨酸-苏氨酸蛋白激酶 mRNA 的表达及其在舌下神经轴切术后的短暂增强”J.Mol.Neurosei.9(1)。
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H.Kondo: "Localization of mRNA for fatty acid transporte protein in developing and mature brain of rats" Mol. Brain Res.46(1). 217-222 (1997)
H.Kondo:“脂肪酸转运蛋白 mRNA 在大鼠发育和成熟大脑中的定位”Mol。
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共 15 条
Analysis of Novel Cellular Functions of Fatty Acid Binding Proteins
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批准号:18390056
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$10.56万
-
财政年份:2006
-
负责人:KONDO Hisatake
-
依托单位:
Functional analysis of fatty acid binding proteins in immune and neural tissues.
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批准号:14370002
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$8.83万
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财政年份:2002
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负责人:KONDO Hisatake
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依托单位:
Molecular and Celluler Biological Analysis of the functional Significance of Phosphoinositide Metabolism
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批准号:11694235
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项目类别:Grant-in-Aid for Scientific Research (A)
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资助金额:$8.51万
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财政年份:1999
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负责人:KONDO Hisatake
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依托单位:
Molecular and Cell Biological Analysis of lipid kinases and phosphatases involved in phosphoinosilide signaling
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批准号:11470001
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项目类别:Grant-in-Aid for Scientific Research (B).
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资助金额:$9.22万
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财政年份:1999
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负责人:KONDO Hisatake
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依托单位:
The regulation mechanism of lipid kinase in the signal transduction
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批准号:09044248
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项目类别:Grant-in-Aid for international Scientific Research
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资助金额:$10.11万
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财政年份:1997
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负责人:KONDO Hisatake
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依托单位:
Moleculap biological and Pustochemical study on phospholipid me tabolic enzymes inviolved in signal transduction
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批准号:07457001
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$4.93万
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财政年份:1995
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负责人:KONDO Hisatake
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依托单位:
Molecular biological and morphological analysis of signal transduction mechanism from membrane to nuchreos
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批准号:07307027
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项目类别:Grant-in-Aid for Scientific Research (A)
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资助金额:$2.75万
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财政年份:1995
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负责人:KONDO Hisatake
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依托单位:
Regulation of inositol phospholipid-pelated 2nd messengers
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批准号:07044216
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项目类别:Grant-in-Aid for international Scientific Research
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资助金额:$7.3万
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财政年份:1995
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负责人:KONDO Hisatake
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依托单位:
Histological study on the gene expression of several proteins related to the intracellular Ca-signals.
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批准号:04404020
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项目类别:Grant-in-Aid for General Scientific Research (A)
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资助金额:$16.64万
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财政年份:1992
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负责人:KONDO Hisatake
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依托单位:
Establishment of embedment-free electron microscopy and analysis of the nature of cytoplasmic matrix by this methodology
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批准号:62480092
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项目类别:Grant-in-Aid for General Scientific Research (B)
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资助金额:$4.29万
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财政年份:1987
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负责人:KONDO Hisatake
-
依托单位: