Histological study on the gene expression of several proteins related to the intracellular Ca-signals.
与细胞内 Ca 信号相关的几种蛋白质的基因表达的组织学研究。
基本信息
- 批准号:04404020
- 负责人:
- 金额:$ 16.64万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (A)
- 财政年份:1992
- 资助国家:日本
- 起止时间:1992 至 1993
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The gene cloning, sequencing and expression localization of several proteins initimately related to the intracellular Ca-signal mechanism were undertaken in the present study. These proteins included calbindin calreticulin, Ca/calmodulin dependent protein kinase(CaMK) II and IV, diacylglycerol kinase(DGK), calpain, calpastatin, 14-3-3 protein (presumptive regulator for Ca-dependent protein kinase C) ; and further protein phosphatases (PP-2A, 2B, 2C), -adrenergic receptor kinase and neuronal and non-neuronal enolases. By in situ hybridization histochemistry using specific cDNA probes for each protein which were obtained by PCR amplification, spatial and temporal heterogeneity in the expression intensity was remarkable in developing and mature rat brain. For example, cerebellar granule cells expressed intensely CaMKII and IV and PP subunits and subtypes, while no significant expression for calbindin and calreticulin was detected in these cells. This heterogeneity suggests the functional heterogeneity of these Ca-signal-related proteins in different neuronal populations, and further suggests the existence of multiple isoforms for some of these proteins. The latter possibility was pursued by using DGK and CaMKIV as targets. As a result, 80 kDa- and 90 kDa-DGKs were identified by the gene cloning. They showed 58% identity to each other and contained zinc finger-like sequences, E-F hand motifs and ATP-binding sites. The gene expression for 80 kDa-DGK was confined to oligodendrocytes, suggesting the involvement of this isoform in Ca-signals related to the myelin formation. The gene of 90 kDa-DGK was expressed predominantly in neurons of the caudate putamen, suggesting its involvement in Ca-signals related the dopaminergic transmission. In addition, a cDNA encoding CaMKIV -subtype was isolated and sequenced from rat brain. Although addition of 28 amino acids in its amino terminal region was the only difference of the -subtype from the *, the diffirential gene expression fo
本研究对几种与细胞内钙信号机制初步相关的蛋白质进行了基因克隆、测序和表达定位。这些蛋白质包括钙结合蛋白钙网蛋白、钙/钙调蛋白依赖性蛋白激酶(CaMK)II和IV、二酰基甘油激酶(DGK)、钙蛋白酶、钙蛋白酶抑制剂、14-3-3蛋白(钙依赖性蛋白激酶C的假定调节剂);以及其他蛋白磷酸酶(PP-2A、2B、2C)、β-肾上腺素能受体激酶以及神经元和非神经元烯醇化酶。通过原位杂交组织化学,使用特定的cDNA探针,通过PCR扩增获得的每个蛋白质,空间和时间的异质性的表达强度显着的发育和成熟的大鼠大脑。例如,小脑颗粒细胞强烈表达CaMK II和IV和PP亚基和亚型,而在这些细胞中检测到钙结合蛋白和钙网蛋白没有显著表达。这种异质性表明这些Ca信号相关蛋白在不同神经元群体中的功能异质性,并进一步表明其中一些蛋白存在多种亚型。后一种可能性是通过使用DGK和CaMKIV作为目标来实现的。结果,通过基因克隆鉴定了80 kDa和90 kDa的DGKs。它们之间的同源性为58%,含有锌指样序列、E-F手结构域和ATP结合位点。80 kDa-DGK的基因表达仅限于少突胶质细胞,表明这种亚型参与与髓鞘形成相关的Ca-信号。90 kDa-DGK基因主要在尾壳核神经元中表达,提示其参与多巴胺能传递相关的Ca ~(2+)信号。此外,从大鼠脑中分离并测序了编码CaMKIV -亚型的cDNA。虽然-亚型与 * 亚型的差异仅在于其氨基末端增加了28个氨基酸,但其差异基因表达与 * 亚型的差异基因表达有关。
项目成果
期刊论文数量(10)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
M.WATANABE,T.NAGAMINE K.SAKIMURA,Y.TAKAHASHI H.KONDO: "Developmental study of the gene expression for X and B subumits of enolase in the rat brain by in situ hybridization histochemistry" Journal Comparative Newology. 326. 1-9 (1992)
M.WATANABE、T.NAGAMINE K.SAKIMURA、Y.TAKAHASHI H.KONDO:“通过原位杂交组织化学对大鼠脑中烯醇酶 X 和 B 亚基的基因表达进行发育研究”杂志比较新闻学。
- DOI:
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- 影响因子:0
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阪上洋行,近藤尚武: "Differential expression of mRNAs encoding γ and δ subunits of Ca^<2+>/calmodulin-dependent protein kinase typeII(CaM kinaseII)in the mature and postnatally developing rat brain" Molecular Brain Research. 20. 51-63 (1993)
Hiroyuki Sakagami、Naotake Kondo:“成熟和出生后发育的大鼠脑中编码 Ca^2+/钙调蛋白依赖性蛋白激酶 II 型 (CaM 激酶 II) 的 γ 和 δ 亚基的 mRNA 的差异表达”《分子脑研究》20. 51。 -63 (1993)
- DOI:
- 发表时间:
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- 影响因子:0
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M.WATANBE,T.ISOBE T.Ichimura,R.KUWANO Y.TAKAHASHI,H.KONDO: "Molecular cloning of pat cDNAs for B and subtypes of 14-3-3 protein and developmental changes in expression of their mRNAs in the nervous system" Molecilar Brain Resedrch. (1992)
M.WATANBE、T.ISOBE T.Ichimura、R.KUWANO Y.TAKAHASHI、H.KONDO:“14-3-3 蛋白 B 和亚型的 pat cDNA 的分子克隆及其 mRNA 在神经细胞中表达的发育变化
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- 影响因子:0
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阪上洋行,近藤尚武: "Cloning and sequencing of a gene encoding the β polypeptide of Ca^<2+>/calmodulin-dependent protein kinaseIV and its expression confined to the mature cerebellar granule cells" Molecular Brain Research. 19. 215-218 (1993)
Hiroyuki Sakagami、Naotake Kondo:“编码Ca 2+ /钙调蛋白依赖性蛋白激酶IV的β多肽的基因的克隆和测序及其在成熟小脑颗粒细胞中的表达”《分子脑研究》19。215-218。 (1993)
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- 影响因子:0
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渡辺雅彦 他4名そして近藤尚武: "Molecular cloning of rat cDNAs for β and γ subtypes of 14-3-3 protein and developmental changes in expression of their mRNAs in the nervous system" Molecular Brain Research. 17. 135-146 (1993)
Masahiko Watanabe 等 4 人以及 Naotake Kondo:“14-3-3 蛋白 β 和 γ 亚型的大鼠 cDNA 的分子克隆及其 mRNA 在神经系统中表达的发育变化”《分子脑研究》17. 135-146( 1993)
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KONDO Hisatake其他文献
KONDO Hisatake的其他文献
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{{ truncateString('KONDO Hisatake', 18)}}的其他基金
Analysis of Novel Cellular Functions of Fatty Acid Binding Proteins
脂肪酸结合蛋白的新细胞功能分析
- 批准号:
18390056 - 财政年份:2006
- 资助金额:
$ 16.64万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Functional analysis of fatty acid binding proteins in immune and neural tissues.
免疫和神经组织中脂肪酸结合蛋白的功能分析。
- 批准号:
14370002 - 财政年份:2002
- 资助金额:
$ 16.64万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular and Celluler Biological Analysis of the functional Significance of Phosphoinositide Metabolism
磷酸肌醇代谢功能意义的分子和细胞生物学分析
- 批准号:
11694235 - 财政年份:1999
- 资助金额:
$ 16.64万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Molecular and Cell Biological Analysis of lipid kinases and phosphatases involved in phosphoinosilide signaling
参与磷酸肌醇信号转导的脂质激酶和磷酸酶的分子和细胞生物学分析
- 批准号:
11470001 - 财政年份:1999
- 资助金额:
$ 16.64万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
The regulation mechanism of lipid kinase in the signal transduction
脂质激酶在信号转导中的调控机制
- 批准号:
09044248 - 财政年份:1997
- 资助金额:
$ 16.64万 - 项目类别:
Grant-in-Aid for international Scientific Research
Molecular and Cell Biological Analysis of Lipid Kinases in Relation to Signaling and Vesicle Traffic.
与信号传导和囊泡运输相关的脂质激酶的分子和细胞生物学分析。
- 批准号:
09470001 - 财政年份:1997
- 资助金额:
$ 16.64万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Moleculap biological and Pustochemical study on phospholipid me tabolic enzymes inviolved in signal transduction
参与信号转导的磷脂代谢酶的分子生物学和化学研究
- 批准号:
07457001 - 财政年份:1995
- 资助金额:
$ 16.64万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular biological and morphological analysis of signal transduction mechanism from membrane to nuchreos
从膜到核的信号转导机制的分子生物学和形态学分析
- 批准号:
07307027 - 财政年份:1995
- 资助金额:
$ 16.64万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Regulation of inositol phospholipid-pelated 2nd messengers
肌醇磷脂包裹的第二信使的调节
- 批准号:
07044216 - 财政年份:1995
- 资助金额:
$ 16.64万 - 项目类别:
Grant-in-Aid for international Scientific Research
Establishment of embedment-free electron microscopy and analysis of the nature of cytoplasmic matrix by this methodology
免包埋电子显微镜的建立及利用该方法分析细胞质基质的性质
- 批准号:
62480092 - 财政年份:1987
- 资助金额:
$ 16.64万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
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