Molecular and Cell Biological Analysis of lipid kinases and phosphatases involved in phosphoinosilide signaling
参与磷酸肌醇信号转导的脂质激酶和磷酸酶的分子和细胞生物学分析
基本信息
- 批准号:11470001
- 负责人:
- 金额:$ 9.22万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B).
- 财政年份:1999
- 资助国家:日本
- 起止时间:1999 至 2000
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
In an in situ hybridization histochemical study on the localization of mRNA for SHIP2, SH2-domain containing inositol 5-phosphatase SHIP isozyme in the brain of developing and mature rats. It was detected in the ventricular germinal zone at embryonic stages. As the postnatal development proceeded, the expression was evident in cells of the white matters, presumptive oligodendrocytes, without significant expression in neurons throughout the development. In another study on the localization of PLD mRNAs, the expression of PLD1 mRNA was detected in presumptive oligodendrocytes, while PLD2 mRNA was expressed mainly in presumptive astrocytes. In addition, the gene expression for PLDs were detected in neuroepithelial cells of the ventricular/ependymal zones and the gene for PLD2 was expressed transiently in early postnatal gray matters, presumptive neurons. In the other study the localization of mRNAs for 10 isoforms of protein kinase C (PKC) in the rat brain was studied at embryonic and postnatal stages. In the embryonic brain, the gene expression was positive only for PKCε, μ, λ, ζ with the former three more evident ; the expression for PKCμ, I in the ventricular germinal zone and that for PKCε, ζ, λ in the mantle zone. In the postnatal brain, the expression for PKCζ, η, θ was detected differentially in a few circumscribed loci such as the thalamus, habenula, septum and cerebellar granule cells, whereas that for the other isoforms was seen widely in various loci of the gray matter with different intensity. The expression in the cerebellar external granule cell layer was positive only for PKCβ, μ, λ with that for PKCβ confined to its inner zone. There is a general tendency for all PKC isoforms that the expression levels reach at peaks in carly postnatal brain and decreases more or less in adult specimens.
用原位杂交组织化学方法研究了SHIP 2、SH 2-结构域的肌醇5-磷酸酶SHIP同工酶在发育和成熟大鼠脑中的mRNA定位。在胚胎期的脑室生发区检测到。随着出生后发育的进行,表达在白色物质的细胞中是明显的,推测为少突胶质细胞,在整个发育过程中在神经元中没有显著表达。在另一项PLD mRNA定位的研究中,PLD 1 mRNA在推定的少突胶质细胞中表达,而PLD 2 mRNA主要在推定的星形胶质细胞中表达。此外,在脑室/室管膜区的神经上皮细胞中检测到PLD的基因表达,并且PLD 2的基因在出生后早期灰质中瞬时表达,推定神经元。在另一项研究中,蛋白激酶C(PKC)的10种亚型的mRNA在胚胎和出生后阶段的大鼠脑中的定位进行了研究。在胚胎脑中,仅PKCε、μ、λ、λ基因表达阳性,前三者表达较明显,PKCμ、I在脑室胚带表达,PKCε、λ、λ在外套带表达。在出生后的脑中,PKC β、η、θ在丘脑、缰核、隔核和小脑颗粒细胞等少数几个特定部位有不同程度的表达,而其他亚型则广泛分布于灰质的不同部位,表达强度不同。小脑外颗粒细胞层仅PKCβ、μ、λ表达阳性,PKCβ仅表达于内带。所有PKC亚型的表达水平在出生后早期均达到高峰,而在成人标本中或多或少地下降。
项目成果
期刊论文数量(30)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
H.Sakagami,M.Umemiya,S.Saito & H.Kondo: "Distinct immunohisto chemical localization of two isoforms of Ca/calmodulin dependent protein kinase kinases in the adult brain"Eur.Journal of Neuroscience. 12(1). 89-99 (2000)
H.坂上,M.梅宫,S.斋藤
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- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
D.H.Jones,J.B.Morris,C.P.Morgan,H.Kondo,R.F.Ivine S.Cockcroft: "Type 1 phosphatidylinositol 4-phosphate 5-kinase directly interacts with ADP-ribosylation factor 1 and is responsible for phosphatidylinositol,4,5-bisphosphate"J.Biological Chemistry. 275(18)
D.H.Jones、J.B.Morris、C.P.Morgan、H.Kondo、R.F.Ivine S.Cockcroft:“1 型磷脂酰肌醇 4-磷酸 5-激酶直接与 ADP-核糖基化因子 1 相互作用,并负责磷脂酰肌醇 4,5-二磷酸”J
- DOI:
- 发表时间:
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- 影响因子:0
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S.Yoshida,H.Sakagami,Y.Owada,H.Kondo: "Localization of PDK-1 mRNA in the brain of developing and adult rats"Tohoku J. Exp. Med. 187-2. 249-255 (1999)
S.Yoshida、H.Sakagami、Y.Owada、H.Kondo:“发育中和成年大鼠大脑中 PDK-1 mRNA 的定位”Tohoku J. Exp。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
H.Kondo: "Molecular biological and histochemical analysis on the functional significance of phosphoinositide metabolism in the nervous system"Genetic Influences on neural and behavioral function, CRC Press. D.W.Pfaff et al. 261-276 (1999)
H.Kondo:“神经系统中磷酸肌醇代谢功能意义的分子生物学和组织化学分析”《遗传对神经和行为功能的影响》,CRC Press。
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- 期刊:
- 影响因子:0
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- 通讯作者:
M.Pokorski,H.Sakagamiki H.Kondo: "Classical protein kinase C and its hypoxic stimulus-induced translocation in the cat and rat carotid body"Eur.Respiratory Journal. 16(2). 459-463 (2000)
M.Pokorski、H.Sakagamiki H.Kondo:“经典蛋白激酶 C 及其缺氧刺激诱导的猫和大鼠颈动脉体易位”Eur.Respiratory Journal。
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- 影响因子:0
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KONDO Hisatake其他文献
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{{ truncateString('KONDO Hisatake', 18)}}的其他基金
Analysis of Novel Cellular Functions of Fatty Acid Binding Proteins
脂肪酸结合蛋白的新细胞功能分析
- 批准号:
18390056 - 财政年份:2006
- 资助金额:
$ 9.22万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Functional analysis of fatty acid binding proteins in immune and neural tissues.
免疫和神经组织中脂肪酸结合蛋白的功能分析。
- 批准号:
14370002 - 财政年份:2002
- 资助金额:
$ 9.22万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular and Celluler Biological Analysis of the functional Significance of Phosphoinositide Metabolism
磷酸肌醇代谢功能意义的分子和细胞生物学分析
- 批准号:
11694235 - 财政年份:1999
- 资助金额:
$ 9.22万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
The regulation mechanism of lipid kinase in the signal transduction
脂质激酶在信号转导中的调控机制
- 批准号:
09044248 - 财政年份:1997
- 资助金额:
$ 9.22万 - 项目类别:
Grant-in-Aid for international Scientific Research
Molecular and Cell Biological Analysis of Lipid Kinases in Relation to Signaling and Vesicle Traffic.
与信号传导和囊泡运输相关的脂质激酶的分子和细胞生物学分析。
- 批准号:
09470001 - 财政年份:1997
- 资助金额:
$ 9.22万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Moleculap biological and Pustochemical study on phospholipid me tabolic enzymes inviolved in signal transduction
参与信号转导的磷脂代谢酶的分子生物学和化学研究
- 批准号:
07457001 - 财政年份:1995
- 资助金额:
$ 9.22万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular biological and morphological analysis of signal transduction mechanism from membrane to nuchreos
从膜到核的信号转导机制的分子生物学和形态学分析
- 批准号:
07307027 - 财政年份:1995
- 资助金额:
$ 9.22万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Regulation of inositol phospholipid-pelated 2nd messengers
肌醇磷脂包裹的第二信使的调节
- 批准号:
07044216 - 财政年份:1995
- 资助金额:
$ 9.22万 - 项目类别:
Grant-in-Aid for international Scientific Research
Histological study on the gene expression of several proteins related to the intracellular Ca-signals.
与细胞内 Ca 信号相关的几种蛋白质的基因表达的组织学研究。
- 批准号:
04404020 - 财政年份:1992
- 资助金额:
$ 9.22万 - 项目类别:
Grant-in-Aid for General Scientific Research (A)
Establishment of embedment-free electron microscopy and analysis of the nature of cytoplasmic matrix by this methodology
免包埋电子显微镜的建立及利用该方法分析细胞质基质的性质
- 批准号:
62480092 - 财政年份:1987
- 资助金额:
$ 9.22万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
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