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Molecular and Cell Biological Analysis of lipid kinases and phosphatases involved in phosphoinosilide signaling

参与磷酸肌醇信号转导的脂质激酶和磷酸酶的分子和细胞生物学分析

基本信息

批准号：
11470001
负责人：
KONDO Hisatake
金额：
$ 9.22万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11470001/
关键词：
in situ hybridization brain white matter SHIP2 PLD PKC PLO リピドキナーゼリピドホスファターゼ PI4キナーゼトランスジェニックマウス SHIP synaptojanin 脳内遺伝子発現

项目摘要

In an in situ hybridization histochemical study on the localization of mRNA for SHIP2, SH2-domain containing inositol 5-phosphatase SHIP isozyme in the brain of developing and mature rats. It was detected in the ventricular germinal zone at embryonic stages. As the postnatal development proceeded, the expression was evident in cells of the white matters, presumptive oligodendrocytes, without significant expression in neurons throughout the development. In another study on the localization of PLD mRNAs, the expression of PLD1 mRNA was detected in presumptive oligodendrocytes, while PLD2 mRNA was expressed mainly in presumptive astrocytes. In addition, the gene expression for PLDs were detected in neuroepithelial cells of the ventricular/ependymal zones and the gene for PLD2 was expressed transiently in early postnatal gray matters, presumptive neurons. In the other study the localization of mRNAs for 10 isoforms of protein kinase C (PKC) in the rat brain was studied at embryonic and postnatal stages. In the embryonic brain, the gene expression was positive only for PKCε, μ, λ, ζ with the former three more evident ; the expression for PKCμ, I in the ventricular germinal zone and that for PKCε, ζ, λ in the mantle zone. In the postnatal brain, the expression for PKCζ, η, θ was detected differentially in a few circumscribed loci such as the thalamus, habenula, septum and cerebellar granule cells, whereas that for the other isoforms was seen widely in various loci of the gray matter with different intensity. The expression in the cerebellar external granule cell layer was positive only for PKCβ, μ, λ with that for PKCβ confined to its inner zone. There is a general tendency for all PKC isoforms that the expression levels reach at peaks in carly postnatal brain and decreases more or less in adult specimens.

用原位杂交组织化学方法研究了SHIP 2、SH 2-结构域的肌醇5-磷酸酶SHIP同工酶在发育和成熟大鼠脑中的mRNA定位。在胚胎期的脑室生发区检测到。随着出生后发育的进行，表达在白色物质的细胞中是明显的，推测为少突胶质细胞，在整个发育过程中在神经元中没有显著表达。在另一项PLD mRNA定位的研究中，PLD 1 mRNA在推定的少突胶质细胞中表达，而PLD 2 mRNA主要在推定的星形胶质细胞中表达。此外，在脑室/室管膜区的神经上皮细胞中检测到PLD的基因表达，并且PLD 2的基因在出生后早期灰质中瞬时表达，推定神经元。在另一项研究中，蛋白激酶C（PKC）的10种亚型的mRNA在胚胎和出生后阶段的大鼠脑中的定位进行了研究。在胚胎脑中，仅PKCε、μ、λ、λ基因表达阳性，前三者表达较明显，PKCμ、I在脑室胚带表达，PKCε、λ、λ在外套带表达。在出生后的脑中，PKC β、η、θ在丘脑、缰核、隔核和小脑颗粒细胞等少数几个特定部位有不同程度的表达，而其他亚型则广泛分布于灰质的不同部位，表达强度不同。小脑外颗粒细胞层仅PKCβ、μ、λ表达阳性，PKCβ仅表达于内带。所有PKC亚型的表达水平在出生后早期均达到高峰，而在成人标本中或多或少地下降。