Molecular biological and morphological analysis of signal transduction mechanism from membrane to nuchreos

从膜到核的信号转导机制的分子生物学和形态学分析

基本信息

批准号：
07307027
负责人：
KONDO Hisatake
金额：
$ 2.75万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07307027/
关键词：
membranous caveolae CD-38 membranous undercoat nuclear import factor immediate early gene diacylglycerol kinase CaM kinase IV protein kinase C コネクシン CD-38 カベオラプロティンキナーゼC カルモジュリンキナーゼIV 核膜孔ターゲッチング複合体 gap-junction 細胞骨格プロティンキ

项目摘要

The expression and regulation of signal-related molecules were analyzed at levels of genes and proteins in cells in-vitro and in-vivo with regard to their relation to the plasma wembrane, cytoskeletons, cytoplasmic matrix and nucleus. Targeted molecules and structural elements analyzed in this study were the membranous caveolae, connexins. CD-38 molecule, membranous undercoats in the plasma membrane ; Ca/calmodulin-dependent protein kinase IV (CaMK-IV), diacylglycerol kinase (DGK), protein kinase C (PKC) and vesicle-associated small G-proteinin in the cytoplasmic matrix ; nuclear import factors and immediate early genes in the nucleus.The sequestration of GPI-anchored proteins, glycosphingolipids and sphingomyelin in the caveolae were shown to be results of cross-linking, although the localization of caveolin there were constitutive. CD-38, a cell surface-antigen having an activity of cyclic ADP-ribose synthase, was localized in glia, but not neurons, suggesting a new way of functional … More exertion of cyclic ADP-ribose synthase in the neuroanl signal transduction. The localization of dystrophin in the membranous undercoat of muscle cells were clarified in details and its topographical relation to cytoskeletons and exoplasmic surface structures were shown in ultrastructure. The heterogeneity in molecular structure and localization was clarified for DGK,CaMK-IV and PKC,and the nuclear localization of DGK-IV and CaMK-IV was revealed. The identification of novel cytoplasmic components and their importance in the nuclear protein-import were clarified in cultured cells. In the nucleus in vivo, changes in the expression of several immediate early genes occurred after various exogenous stimuli such as pain. A novel immediately early gene was identified from the hippocampus, implicating its functional involvement in synaptic plasticity. A small G-protein, rab 12 is shown to be involved in the secretory vesicle transport in atrial muscle cells. In conclusion, all these findings are of extreme importance in understanding the signal transduction mechanism in relation to cellular ultrastructure. Less

关于信号相关分子的表达和调节在细胞中的基因和蛋白质水平和体内的蛋白质水平上与血浆Wembrane的关系，细胞骨架，细胞质基质和细胞核的关系。在这项研究中分析的靶向分子和结构元素是膜状小窝，连接蛋白。 CD-38分子，质膜中的膜不足； Ca/CA/钙调蛋白依赖性蛋白激酶IV（CAMK-IV），二酰基甘油激酶（DGK），蛋白激酶C（PKC）和小囊泡相关的小G蛋白质中的小G蛋白素在细胞质基质中；核进口因子和核中的早期基因在核进口因子和早期基因的序列。尽管小窝蛋白，糖磷脂和鞘磷脂在小窝中的序列被证明是交叉链接的结果，尽管小窝蛋白的定位是组成型的。 CD-38是具有环状ADP-核糖合酶活性的细胞表面抗原，它位于神经胶质中，但不定位于神经元中，这表明一种新的功能性……更多地施加了神经ANLOANL信号转导中环状ADP-ribose Synthase的施加。详细阐明了肌营养不良蛋白在肌肉细胞膜不底漆中的定位，并在超微结构中显示了其与细胞骨架的地形关系和外部表面结构。对于DGK，CAMK-IV和PKC，阐明了分子结构和定位的异质性，并且揭示了DGK-IV和CAMK-IV的核定位。在培养的细胞中阐明了新型细胞质成分及其在核蛋白象征中的重要性。在核心体内，几种直接早期基因的表达发生变化发生在各种外源刺激（例如疼痛）之后。从海马体中鉴定出一种新的早期基因，暗示其在突触可塑性方面的功能投资。一个小的G蛋白Rab 12被证明与心房肌肉细胞中的秘密场地运输有关。总之，所有这些发现对于了解与细胞超微结构有关的信号传递机制至关重要。较少的