Research on the treatment of inflammatory bowel diseases using Reg gene modified mice

Reg蛋白是胰腺、肾脏和胃肠道粘膜中表达的生长因子。我们以前发现Reg蛋白刺激胃肠道上皮的生长。目前还没有关于Reg蛋白在体内对胃肠道粘膜的影响的资料。作为体内模型，我们使用了带有CMV启动子和PS2启动子的regg转基因小鼠。我们还使用了Reg敲除小鼠。通过对regg转基因小鼠的研究，我们发现regg蛋白在胃上皮细胞增殖和向胃底腺分化中起重要作用。此外，研究发现Reg蛋白在胃粘膜损伤后的胃上皮修复中起关键作用。通过对regg -knuckout小鼠的分析，我们发现Reg蛋白对肠绒毛的发育和维持起着重要的作用。重组Reg蛋白经肛门给药于实验性结肠炎模型动物，发现Reg蛋白能增强肠上皮的增殖，加速实验性结肠炎模型动物上皮损伤的修复。这些结果提示Reg蛋白对炎症性肠病可能有治疗作用。

Reg protein is a growth factor expressed in pancreas, kidney, and in gastrointestinal mucosa. We have previonoly found that Reg protein stimulates the growth of gastrointestinal epithelium. There was no data available concerning the in vivo effect of Reg protein on the gastrointestinal mucosa. As in vivo models, we have employed Reg-transgenic mice with CMV promoter and with PS2 promoter. We also employed Reg knockout mice. From the studies using Reg-transgenic mice, we have found that Reg protein is important for the proliferation of gastric epithelium and for the differentiation to gastric fundic glands. In addition, Reg protein was found to have pivotal role in the gastric epithelial repair from gastric mucosal injury. From the avolysis of reg-knuckout mice, we have found that Reg protein is important for the development and maintenance of intestinal villi. When recombinant Reg protein was trans-anally administered to model animals with experimental colitis, Reg protein was found to augment the proliferation of intestinal epithelium and accelerate the repairment of experimentally-induced epithelial damage.All of these results suggested the possible therapeutic effect of Reg protein for the inflammatory bowel diseases.