Reg-mediatedproliferative effect of gastrin on gastric epithelial cell and cancer cell

本研究旨在验证胃泌素通过增加肠嗜铬细胞（ECL细胞）中Reg蛋白的产生间接刺激胃上皮细胞增殖的假说。为此目的，在作为实验模型的大鼠上进行了体内和体外研究。慢性和急性血浆胃泌素浓度升高可显著增加胃ECL细胞Reg mRNA和Reg蛋白的含量。胃泌素的这种作用几乎完全被胃泌素受体拮抗剂的给药阻断。刺激Reg基因表达，可促进腺颈增殖区胃上皮细胞的轮廓比例。重组reg蛋白加入大鼠胃增殖上皮细胞培养物中，剂量依赖性地刺激细胞增殖。这些数据清楚地表明，胃泌素通过ECL细胞增加Reg蛋白的产生来刺激胃上皮细胞增殖。

The research was performed to test the hypothesis that gastrin Indirectly stimulates the proliferation of gastric epithelial cells through the increased production of Reg protein by entero chromaff in likecells (ECL cells) . For this purpose, in vivo and in vitro studies were done on rats as an experimental model. The chronic and acute elevation of plasma gastrin concentration in rats remarkably dugmented the Reg mRNA and Reg protein contents in gastric ECL cell. This effect of gastrin was almost completely blocked by the administration of a gastrin receptor antagonist. When the gene expression of Reg was stimulated, the profile ration of gastric epithelial cell in gland neck proliferation zone was also stimulated. Recombinant reg protein, when added in the cell culture of rat gastric proliferating epithelial cells, dose-dependently stimulated the proliferation of the cells. These data clearly showed that gastrin stimulates the gastric epithelial proliferation through the increased production of Reg protein by ECL cells.