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in situ Tissue Engineering on digestive systems.

消化系统的原位组织工程。

基本信息

批准号：
14370383
负责人：
NAKAMURA Tatsuo
金额：
$ 9.54万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

Research for the regeneration of the gastro-intestinal tract with in situ Tissue Engineering.In this research program, we tried to regenerate the whole layers of digestive system using in situ Tissue Engineering technique. Targeted gastro-intestinal tract is composed of four layers ; the mucous epithelium, the sub-mucosal connective tissue, the muscle, and the serosa. The glands which produce digestive juice and the autosomal nerves are situated in these four layers. As a scaffold, collagen sponge and the PGA unwoven fabric were applied to the site of the regeneration. We tried repair of the defect of the stomach, the small intestine, the colon, the common bile duct of canine experimental model. We have already observed the early regeneration of the defective mucous epithelium, the connective tissue, and the glands in this system. However, the muscle layers (mainly the smooth muscle) failed to be regenerated. To solve this problem, we had added the growth factors (FGE,TGF β) but the effect was not obvious.This may because of absence of the stem cell as well as poor blood supply. Therefore, in the last year of this project, the progenitor cells were isolated from the host muscle tissue and consequently proliferated in vitro. Then, we tried to apply these proliferated cells with vascular inductive growth factor such as VEGF to promote the regeneration of muscle tissue. Using this technique we confirmed that the muscle-like tissue could be produced in the lower extremities of rabbits.We bridged the nerve defect of the intraabdominal autosomal nerve with artificial biodegradable nerve guide tube, in which the feasibility of the PGA-collagen tube as a nerve guide were strongly indicated. Thus, apparently, even in large mammalians such as beagle dog, it seems highly promizing to make a digestive tissue with a technique of in situ Tissue Engineering.

原位组织工程胃肠道再生的研究本研究尝试利用原位组织工程技术进行消化系统全层再生。靶向胃肠道由四层组成：粘膜上皮、粘膜下结缔组织、肌肉和浆膜。产生消化液的腺体和常染色体神经位于这四层。作为支架，胶原海绵和PGA无纺布应用于再生部位。本实验采用犬胃、小肠、结肠、胆总管缺损的实验模型进行修复。我们已经观察到在这个系统中有缺陷的粘膜上皮、结缔组织和腺体的早期再生。然而，肌肉层（主要是平滑肌）未能再生。为解决这一问题，我们曾在移植物中加入生长因子（FGE、TGF β），但效果不明显，这可能是由于缺乏干细胞和血液供应不足的原因。因此，在该项目的最后一年，从宿主肌肉组织中分离出祖细胞，并在体外增殖。然后，我们尝试将这些增殖的细胞与血管诱导生长因子如VEGF一起应用，以促进肌肉组织的再生。本实验证实了在家兔下肢可产生肌肉样组织，并以生物可降解神经导管桥接腹内常染色体神经缺损，证明了PGA-胶原管作为神经导管的可行性。因此，显然，即使在大型动物如比格犬中，用原位组织工程技术制造消化组织似乎也是非常有前途的。

项目成果

期刊论文数量（240）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Capillary Endothelial Thrombomodulin Expression and Fibrin Deposition in Rats with Continuous and Bolus Lipopolysaccharide Administration

DOI：
10.1097/01.lab.0000080606.96797.a5
发表时间：
2003-08
期刊：
Laboratory Investigation
影响因子：
5
作者：
Y. Terada;Y. Eguchi;S. Nosaka;T. Toba;Tatsuo Nakamura;Y. Shimizu
通讯作者：
Y. Terada;Y. Eguchi;S. Nosaka;T. Toba;Tatsuo Nakamura;Y. Shimizu

気道系の再生

呼吸系统的再生

DOI：
发表时间：
2002
期刊：
気管支学 24
影响因子：
0
作者：
清水慶彦
通讯作者：
清水慶彦

In situ Tissue Engineeringによる歯周組織の再生

原位组织工程牙周组织再生

DOI：
发表时间：
2003
期刊：
Inflammation and Regeneration 23
影响因子：
0
作者：
Sudo R;et al.;中原貴
通讯作者：
中原貴

気管気管支の再生治療

气管支气管再生治疗

DOI：
发表时间：
2003
期刊：
THE LUNG perspectives 11(1)
影响因子：
0
作者：
Mizuguchi T;Katsuramaki K;Nobuoka T;Kawamoto M;Oshima H;Kawasaki H;Kikuchi H;Shibata C;Hirata K.;中村達雄
通讯作者：
中村達雄

Experimental research on peripheral nerve regeneration using PGA-collagen nerve conduit filled with collagen sponge.

胶原海绵填充PGA-胶原神经导管周围神经再生的实验研究