Development of canine iPS cells and new in situ Tissue Engineering for airway reconstruction

犬 iPS 细胞的开发和用于气道重建的新原位组织工程

• 批准号: 22659250
• 负责人: NAKAMURA Tatsuo
• 金额: $ 2.04万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22659250/
• 关键词: iPS細胞; 人工気管; コラーゲン; 人工臓器; 生体内再生; ステント; 再生医学; 気管; 気道; 再生; 組織工学; 胸部外科; 呼吸器; 肺癌; iPS; 組織幹細胞; 機能再建

We challenged to make an iPS cell from a somatic cell of beagle dog using 4 Yamanaka factors(Oct3, Sox2, Klf4, c-Myc) that were induced by retrovirus, however constant silencing has not yet been recognized.With regard to the regeneration scaffold for airway, new tracheal prosthesis was manufactured with bioabsorbable vicryl mesh reinforced with polypropylene stent with a diameter of 1 mm. In animal experiment in canine, it was determined that conquer of dehiscence between mesh and stent is of importance after the implantation.

我们挑战使用4种Yamanaka因子从比格犬的体细胞制备iPS细胞（Oct 3，Sox 2，Klf 4，c-Myc）的基因沉默，但持续沉默尚未被认识。在气道再生支架方面，采用直径为1 mm的聚丙烯支架增强可吸收薇乔网片，制成新型人工气管。已经确定，在植入后克服网片和支架之间的开裂是重要的。

项目成果

Expression of side population cells during wound healing of rat vocal folds

大鼠声带伤口愈合过程中侧群细胞的表达

• 发表时间: 2010
• 作者: Kojima T

Investigation of optimized conditions for RNA-based cellular reprogramming

基于 RNA 的细胞重编程优化条件的研究

• 发表时间: 2011
• 作者: Shimada;H.;Hashimoto;Y.;Nakada;A.;Shigeno;K.;Nakamura;T
• 通讯作者: T

脂肪由来幹細胞を用いた食道ESD後狭窄の予防

使用脂肪干细胞预防 ESD 后食管狭窄

• 发表时间: 2010
• 作者: 本多通孝;堀義生;中村達雄;山本一道;中田顕;塩谷伊毅;小林丈士;島田英徳;木田直樹
• 通讯作者: 木田直樹

Transplantation of adipose-derived stromal cells prevent esophageal stricture after endoscopic mucosal resection in canine model

脂肪源性基质细胞移植可预防犬模型内镜下粘膜切除术后食管狭窄

• 发表时间: 2010
• 作者: Honda;M.;Hori;Y.;Nakada;A.;Uji;M.;Nishizawa;Y.;Yamamoto;K.;Kobayashi;T.;Shimada;H.;Kida;N.;Sato;T.;Nakamura;T.
• 通讯作者: T.

Potential of Induced Pluripotent Stem Cells for the Regeneration of the Tracheal Wall

• DOI: 10.1177/000348941011901009
• 发表时间: 2010-10-01
• 期刊: ANNALS OF OTOLOGY RHINOLOGY AND LARYNGOLOGY
• 影响因子: 1.4
• 作者: Imaizumi, Mitsuyoshi;Nomoto, Yukio;Omori, Koichi
• 通讯作者: Omori, Koichi