Is stem cell present in human endometrium?

人类子宫内膜中存在干细胞吗？

• 批准号: 14370522
• 负责人: MURAKAMI Takashi
• 金额: $ 4.42万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370522/
• 关键词: endometrium; endometriosis; clonality; 子宮平滑筋; 幹細胞; クロナリティ; レーザーマイクロダイゼクション

Objective : To investigate the clonality of ectopic gland cells in peritoneal endometriosisDesign : Prospective studySetting : University hospitalPatient(s) : Seven-teen women with surgically diagnosed endometriosis.Intervention(s) : Samples of peritoneal endometriotic lesions were obtained from patients during laparoscopic surgery.Main Outcome Measure(s) : Clonality analysis using the laser microdissection technique, a phosphoglycerate kinase (PGK) gene polymorphism assay, and an androgen receptor (AR) gene polymorphism assay following digestion of the DNA with methylation-sensitiveendonuclease were used.Result(s) : Each ectopic gland of the peritoneal endometriotic lesion showed a monoclonal pattern in both the PGK gene and AR gene assays, but the methylation pattern of the PGK gene and/or AR gene was divergent among adjacent glands in the lesion. These data indicate that the peritoneal endometriotic lesions are multicellular in origin, although individual glands of the lesion are derived from single precursor cells.Conclusion(s) : The colored peritoneal endometriotic lesion in the present study was multicellular in origin. Peritoneal endometriotic lesions may thus be initiated by transplantation of a cluster of eutopic endometrial tissues into the pelvis.

目的：探讨异位腺细胞在腹膜异位内膜中的克隆性。设计：前瞻性研究背景：大学附属医院患者(S)：7例经手术证实的子宫内膜异位症患者。干预(S)：腹膜异位内膜病变标本取自腹腔镜手术患者。主要观察指标(S)：采用激光显微解剖技术进行克隆性分析、磷酸甘油酸激酶(PGK)基因多态分析、雄激素受体(AR)基因多态分析。结果(S)但PGK基因和/或AR基因的甲基化模式在病变的相邻腺体之间存在差异。这些数据表明，尽管单个腺体起源于单个前体细胞，但腹膜子宫内膜异位病变的起源是多细胞的。结论：本研究的彩色腹膜子宫内膜异位病变是多细胞起源的。因此，腹膜异位内膜病变可以通过将在位内膜组织簇移植到骨盆内而开始。

项目成果

Analysis of the clonality of ectopic glands in peritoneal endometriosis using laser microdissection.

• DOI: 10.1016/s0015-0282(03)01181-6
• 发表时间: 2003-11
• 期刊: Fertility and sterility
• 影响因子: 6.7
• 作者: H. Nabeshima;T. Murakami;K. Yoshinaga;Kazuyo Sato;Y. Terada;K. Okamura

Nabeshima H, Murakami T, Yoshinaga K, Sato K, Terada Y, Okamura K: "Analysis of the clonality of ectopic glands in peritoneal endometriosis using laser microdissection"Fertility and Sterility. 80(5). 1144-1150 (2003)

Nabeshima H、Murakami T、Yoshinaga K、Sato K、Terada Y、Okamura K：“使用激光显微切割分析腹膜子宫内膜异位症异位腺体的克隆性”生育力和不育。