Is stem cell present in human endometrium?

目的：目的：研究腹腔镜手术中异位腺细胞的克隆性设计：前瞻性研究地点：大学医院患者：17名经手术诊断为子宫内膜异位症的女性。干预措施：腹腔镜手术中从患者身上获取腹膜异位病变样本。主要结果测量：使用激光显微切割技术进行克隆性分析，磷酸甘油酸激酶（PGK）基因多态性分析，雄激素受体（AR）基因多态性分析采用甲基化敏感性核酸内切酶酶切法。在PGK基因和AR基因检测中，腹膜增生性病变的每个异位腺体均显示单克隆模式，但PGK基因和/或AR基因甲基化模式在病变中相邻腺体之间存在差异。这些数据表明，腹膜增生性病变是多细胞的起源，虽然个别腺体的病变是来自单一的前体cells. Conclusions（s）：彩色腹膜增生性病变在本研究中是多细胞的起源。因此，腹膜子宫内膜异位病变可能是通过将一簇在位子宫内膜组织移植到骨盆中而引发的。

Objective : To investigate the clonality of ectopic gland cells in peritoneal endometriosisDesign : Prospective studySetting : University hospitalPatient(s) : Seven-teen women with surgically diagnosed endometriosis.Intervention(s) : Samples of peritoneal endometriotic lesions were obtained from patients during laparoscopic surgery.Main Outcome Measure(s) : Clonality analysis using the laser microdissection technique, a phosphoglycerate kinase (PGK) gene polymorphism assay, and an androgen receptor (AR) gene polymorphism assay following digestion of the DNA with methylation-sensitiveendonuclease were used.Result(s) : Each ectopic gland of the peritoneal endometriotic lesion showed a monoclonal pattern in both the PGK gene and AR gene assays, but the methylation pattern of the PGK gene and/or AR gene was divergent among adjacent glands in the lesion. These data indicate that the peritoneal endometriotic lesions are multicellular in origin, although individual glands of the lesion are derived from single precursor cells.Conclusion(s) : The colored peritoneal endometriotic lesion in the present study was multicellular in origin. Peritoneal endometriotic lesions may thus be initiated by transplantation of a cluster of eutopic endometrial tissues into the pelvis.