Effect of neurotrophic factors on regeneration of denervated recurrent laryngeal nerve

神经营养因子对失神经喉返神经再生的影响

• 批准号: 14370547
• 负责人: YUMOTO Eiji
• 金额: $ 7.42万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370547/
• 关键词: Paralysis of the recurrent laryngeal nerve; Nerve growth factor; Ciliary neurotrophic factor; Basic fibroblast growth factor; Regeneration of nerve fibers

Three kinds of neurotrophic factors were evaluated in adult rats for their effects on regeneration of the denervated recurrent laryngeal nerve (RLN). After transection of the left RLN, a silicone tube was placed between the nerve stumps, and the gap of the nerves was filled with one of the following solutions ; nerve growth factor (NGF), ciliary neurotrophic factor (CNTF), basic fibroblast growth factor (bFGF) and phosphate buffer (PBS) for control. Electromyographical and histological evaluations were conducted either 3 weeks or 5 weeks after the manipulation.Local administration of NGF or bFGF facilitated recovery of the evoked electromyographic response at 3 weeks. CNTF and bFGF increased the total number of regerating nerve fibers at 3 weeks. Recovery of the myelinated nerve fibers were facilitated by application of bFGF. NGF and bFGF were effective in the prevention of the decrease in the size of the motor neuron in the nucleus ambiguus due to transection.

在成年大鼠上观察了三种神经营养因子对失神经喉返神经(RLN)再生的影响。左侧RLN切断后，在神经残端之间放置硅胶管，神经间隙填充神经生长因子(NGF)、睫状神经营养因子(CNTF)、碱性成纤维细胞生长因子(BFGF)和磷酸盐缓冲液(PBS)作为对照。术后3周或5周进行肌电和组织学检查，局部应用NGF或bFGF可促进术后3周诱发肌电反应的恢复。CNTF和bFGF3周时可使再生神经纤维总数增加。应用碱性成纤维细胞生长因子可促进有髓神经纤维的恢复。神经生长因子和碱性成纤维细胞生长因子对延髓疑核运动神经元体积缩小有明显的预防作用。

项目成果

Yumoto E, et al.: "Three-dimensional characteristics of the larynx with immobile vocal fold"Archives of Otolaryngology-Head and Neck Surgery. (In press).

Yumoto E 等人：“声带不动的喉部的三维特征”耳鼻咽喉头颈外科档案。

Yumoto E, et al.: "Causes of recurrent laryngeal nerve paralysis"Auris Nasus Larynx. 29:1. 41-45 (2002)

Yumoto E 等人：“喉返神经麻痹的原因”Auris Nasus Larynx。

河北 誠二, 他: "挿管性反回神経麻痺の臨床的検討"耳鼻咽喉科臨床. 95:9. 945-949 (2002)

Seiji Kawakita 等：“插管引起的喉返神经麻痹的临床回顾”Otolaryngology Clinic 95:9 (2002)。

Yumoto E, et al.: "Causes of recurrent laryngeal nerve palsy"Auris Nasus Larynx. 29. 41-45 (2002)

Yumoto E 等：“喉返神经麻痹的原因”Auris Nasus Larynx。

小山田幸夫, 他: "一側声帯麻痺患者における発声時軟骨間距離の定量的解析"喉頭. 14. 5-10 (2002)

Yukio Oyamada 等人：“单侧声带麻痹患者发声时软骨间距离的定量分析”Laryngeal，14. 5-10 (2002)。