Effect of neurotrophic factors on regeneration of denervated recurrent laryngeal nerve

研究了三种神经营养因子对成年大鼠失神经喉返神经再生的影响。横断左侧RLN后，在神经残端之间放置硅胶管，用以下溶液之一填充神经间隙；神经生长因子（NGF）、睫状神经营养因子（CNTF）、碱性成纤维细胞生长因子（bFGF）和磷酸盐缓冲液（PBS）作为对照。操作后3周或5周分别进行肌电图和组织学评价。局部给予NGF或bFGF有助于3周时诱发肌电反应的恢复。CNTF和bFGF在3周时增加了再生神经纤维的总数。应用bFGF可促进有髓神经纤维的恢复。NGF和bFGF均能有效预防歧义核中运动神经元因横断而减小。

Three kinds of neurotrophic factors were evaluated in adult rats for their effects on regeneration of the denervated recurrent laryngeal nerve (RLN). After transection of the left RLN, a silicone tube was placed between the nerve stumps, and the gap of the nerves was filled with one of the following solutions ; nerve growth factor (NGF), ciliary neurotrophic factor (CNTF), basic fibroblast growth factor (bFGF) and phosphate buffer (PBS) for control. Electromyographical and histological evaluations were conducted either 3 weeks or 5 weeks after the manipulation.Local administration of NGF or bFGF facilitated recovery of the evoked electromyographic response at 3 weeks. CNTF and bFGF increased the total number of regerating nerve fibers at 3 weeks. Recovery of the myelinated nerve fibers were facilitated by application of bFGF. NGF and bFGF were effective in the prevention of the decrease in the size of the motor neuron in the nucleus ambiguus due to transection.