Molecule genetic and molecule immunologic investigations on the mechanisms of viral ocular inflammation

人类腺病毒是腺病毒科乳突腺病毒属的一组病毒，包括51种血清型。根据它们的生物学、免疫学和致癌特性，它们被分为6种，人类adva到-F。多元化组织趋向性发出人类放置在人类之已被证明导致肠,上层。塔呼吸道感染、出血性膀胱炎和流行性角膜结膜炎。人腺病毒4型（AdV-4）是流行性角膜结膜炎患者分离到的主要血清型之一。2001年，我们在日本北部札幌一家医院的眼科病房遇到了流行性角膜结膜炎的院内感染。从医院感染患者中分离腺病毒，血清型特异性抗血清中和试验鉴定为AdV-4。然而，采用聚合酶链反应-限制长度片段多态性方法对其进行分析，发现其与AdV-4原型菌株的切割模式不同。当使用全病毒基因组与Bam HI和Sma I进行比较时，分离物的切割模式与AdV-4p和其他已知的AdV-4变体的切割模式不同。分离株纤维基因的核苷酸序列在GenBank中与AdV-4具有较高的同源性（94.3%），并与原型菌株AdV-4形成单系集群。我们认为，2001年的医院感染是由一种新的AdV-4基因组型引起的，该基因组型被命名为AdV-4c。

Human adenoviruses in the genus Mastadenovirus of the family Adenoviridae form a group of viruses including 51 serotypes. They have been clssified into six species, human AdV-A to -F, according to their biologic, immunologic, and oncogenic properties. A diverse tissue tropism emits within human AdV. The human AdVs have been shown to cause enteric, upper. and tower-respiratry tract infections, hemorrhagic cystitis and epidemic keratoconjunctivitis. Human adenovirus type 4 (AdV-4) was one of the major serotypes isolated from patients with epidemic keratoconjunctivitis. In 2001, we encountered nosocomial infections with epidemic keratoconjunctivitis in the ophthalmology ward of one hospital in Sapporo, in the northern part of Japan. Adenoviruses were isolated from the patients with this nosocomial infection, and they were identified as AdV-4 by neutralization test using serotype-specific antiserum. The isolates, however, showed different cleavage patterns when they were analyzed by polymerase chain reaction-restriction length fragment polymorphism method targeting partial hexon region in parallel comparison with those of AdV-4 prototype strain. When the cleavage patterns of the isolates were compared using full viral genome with Bam HI and Sma I, they are different from hose of AdV-4p and other previously known AdV-4 variants. The nucleotide sequences of the fiber gene of the isolates showed higheat homologies (94.3%) with AdV-4 among the available nucleotide sequences from GenBank, and formed a monophyletic cluster along with the prototype strain AdV-4. The isolates, however were located as the different lineage of AdV-4 from sporadic infectious We concluded that the nosocomial infection in 2001 was caused by a new genome type of AdV-4, which was designated as AdV-4c.