Molecule genetic and molecule immunologic investigations on the mechanisms of viral ocular inflammation

腺病毒科乳腺腺病毒属中的人腺病毒形成包括51个血清型的一组病毒。根据其生物学、免疫学和致癌特性，已将其分为六种，即人AdV-A至人AdV-F。人AdV具有不同的组织嗜性。以及塔-尿道感染、出血性膀胱炎和流行性角结膜炎。人腺病毒4型（AdV-4）是从流行性角结膜炎患者中分离的主要血清型之一。2001年，我们在日本北方札幌的一家医院的眼科病房遇到了流行性角结膜炎的医院感染。从该医院感染的患者中分离腺病毒，用腺病毒型特异性抗血清进行中和试验，鉴定为腺病毒4型。然而，分离株，显示不同的切割模式时，他们通过聚合酶链反应-限制性长度片段多态性方法分析，针对部分六邻体区域的平行比较与腺病毒-4原型株。当使用全病毒基因组与BamH I和Sma I比较时，分离物的切割模式不同于AdV-4p和其它先前已知的AdV-4变体的切割模式。在GenBank中已获得的核苷酸序列中，其纤维蛋白基因的核苷酸序列与AdV-4的同源性高达94.3%，并与AdV-4形成了沿着的单系群。结论：2001年的医院感染是由一种新的腺病毒4型引起的，命名为腺病毒4c。

Human adenoviruses in the genus Mastadenovirus of the family Adenoviridae form a group of viruses including 51 serotypes. They have been clssified into six species, human AdV-A to -F, according to their biologic, immunologic, and oncogenic properties. A diverse tissue tropism emits within human AdV. The human AdVs have been shown to cause enteric, upper. and tower-respiratry tract infections, hemorrhagic cystitis and epidemic keratoconjunctivitis. Human adenovirus type 4 (AdV-4) was one of the major serotypes isolated from patients with epidemic keratoconjunctivitis. In 2001, we encountered nosocomial infections with epidemic keratoconjunctivitis in the ophthalmology ward of one hospital in Sapporo, in the northern part of Japan. Adenoviruses were isolated from the patients with this nosocomial infection, and they were identified as AdV-4 by neutralization test using serotype-specific antiserum. The isolates, however, showed different cleavage patterns when they were analyzed by polymerase chain reaction-restriction length fragment polymorphism method targeting partial hexon region in parallel comparison with those of AdV-4 prototype strain. When the cleavage patterns of the isolates were compared using full viral genome with Bam HI and Sma I, they are different from hose of AdV-4p and other previously known AdV-4 variants. The nucleotide sequences of the fiber gene of the isolates showed higheat homologies (94.3%) with AdV-4 among the available nucleotide sequences from GenBank, and formed a monophyletic cluster along with the prototype strain AdV-4. The isolates, however were located as the different lineage of AdV-4 from sporadic infectious We concluded that the nosocomial infection in 2001 was caused by a new genome type of AdV-4, which was designated as AdV-4c.