Pathogenic mechanisms of apical periodontal diseases : Kinetics of dendritic cells and immure functional molecules

根尖周病的发病机制：树突状细胞和免疫功能分子的动力学

• 批准号: 14370616
• 负责人: OKIJI Takashi
• 金额: $ 5.95万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370616/
• 关键词: apical periodontitis; dendritic cell; immunohistochemistry; macrophage; immune functional molecules; periodontal ligament; ultrastructure; MHCクラスII分子

This study aimed to investigate the role of dendritic cells in the development of apical periodontitis. Experimental periapical lesions were induced in rat molars by making unsealed pulp exposures, and ultrastructure, distributional density and immune functional molecule expression of dendritic cells were investigated by means of inmunoelectron microscopy. During the period of active lesion expansion (expanding stage), macrophages were dominant and only a small number of dendritic cells were detected. Following lesion stabilization (chronic stage), however, most of MHC class II molecule-expressing cells were identified as dendritic cells. Cell-to-cell contact between dendritic cells and lymphocytes was sometimes seen in the chronic stage. Morphological change of dendritic cells was also noted : cells with thin and short cytoplasmic processes and poorly developed organelles were predominant in the expanding stage, whereas large cells with long cytoplasmic processes were predominant in the chronic stage. Moreover, dendritic cells in the chronic stage were divided into two subpopulations according to the immunoreactivity to CD11c and OX62 : although most dendritic cells expressed CD11c, there were minor but definite subpopulation which expressed OX62 and had a small and round cell body with a small number of short cytoplasmic processes. These findings suggested that dendritic cells, composed of different subpopulations according to the stage of maturation/activation, are involved in lesion chronicity by acting as antigen presenting cells in response to chronic antigenic challenge from infected root canals.

这项研究旨在研究树突状细胞在根尖牙周炎发展中的作用。通过进行未密封的纸浆暴露，通过持续性核心显微镜研究了树突状细胞的超微结构，分布密度和免疫功能分子表达，从而在大鼠磨牙中诱导了实验性根尖细胞病变。在活动病变扩张（扩展阶段）的期间，巨噬细胞占主导地位，仅检测到少量的树突状细胞。但是，在病变稳定（慢性阶段）之后，大多数表达MHC II类分子的细胞被鉴定为树突状细胞。在慢性阶段，有时会发现树突状细胞和淋巴细胞之间的细胞对细胞接触。还注意到树突状细胞的形态变化：在膨胀阶段，具有较薄和短的细胞质过程和较短的细胞质过程和发育不良的细胞器的细胞主要是主导的，而在慢性阶段，具有较长细胞质过程的大细胞占主导地位。此外，根据对CD11C和OX62的免疫反应性，将慢性阶段的树突状细胞分为两个亚群：尽管大多数树突状细胞表达了CD11C，但有较小但明确的亚群表达OX62，并且具有小细胞体和少量的短细胞质过程。这些发现表明，根据成熟/激活的阶段，由不同亚群组成的树突状细胞通过充当抗原呈现细胞而响应感染根管的慢性抗原攻击而参与病变慢性。

项目成果

新編口腔組織発生学

新版口腔组织胚胎学

• 发表时间: 2005
• 作者: Chowdhury SA;Kishino K;Satoh R et al.;興地隆史
• 通讯作者: 興地隆史

根尖歯周組織の生体防御・修復機構

根尖牙周组织的生物防御和修复机制

• 发表时间: 2005
• 期刊: The Quintessence 24(印刷中)
• 作者: 高山直士;田島雅道;菊地寛高;西川博文;坂上宏ら;興地隆史
• 通讯作者: 興地隆史

興地隆史: "結合素子としての歯髄"The Quintessence. 22. 135-144 (2003)

Takashi Kochi：“牙髓作为连接元素”The Quintessence 22. 135-144 (2003)。

Aging phenomenon of the dentin and dental pulp

牙本质和牙髓的老化现象

• 发表时间: 2004
• 期刊: Clinical Calcium Vol.14
• 作者: Ikeda H;Suda H.;Hayashi M. et al.;坂上宏;Ikeda H;T.Okiji
• 通讯作者: T.Okiji

ラット実験的根尖性歯周炎における樹状細胞およびマクロファージの多様性に関する電顕免疫組織化学的研究

大鼠实验性根尖周炎树突状细胞和巨噬细胞多样性的电镜免疫组化研究

• 发表时间: 2004
• 期刊: 日本歯科保存学雑誌 47
• 作者: Lee;J.;Adachi;K.;Gionhaku;N.;Fujita;S.;Uchida;T.;Gerstner;G.E.;Koshikawa;N.;金子友厚
• 通讯作者: 金子友厚