Neuro-immune connection in deseased dental pulp-an immunohistochemical study

病变牙髓中的神经免疫联系——免疫组织化学研究

• 批准号: 07672070
• 负责人: OKIJI Takashi
• 金额: $ 1.47万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07672070/
• 关键词: dental pulp; intradental nerves; class II MHC antigen-expressing cells; dental caries; pulpitis; neuropeptides; immunohistochemistry; dendritic cell; クラスII抗原陽性細胞; マクロファージ; う蝕; 神経免疫相互作用

This sutdy focused on peptidergic nerve fibers and class II major histocompatibility compolex (MHC) antigen-expresing cells of the dental pulp and examined whether there are some spatial relationships in the arrangement of these structures under physiological and pathological conditions. Effects of neuropeptides on functional properties of pulpally-derived class II MHC antigen-expressing cells were also analyzed in vitro. The results obtained were as follows :1. Immunohistochemistrty and confocal laser scanninb microscopy revealed that class II MHC antigen-expressing cells of the normal humah and rat dental pulp are frequently distributed in close proximity to blood vessels and nerve fibers.2. In human teeth bearing dentinal caries and rat molars subjected to experimental caries induction, a localized accumulation of class II MHC antigen-expressing cells was markedly observed along the pulp-dentin border subjacent to the pulpal end of the dentinal tubules communicating with the carious lesion. A further analysis on the human carious teeth demonstrated that neuropeptide-containing nerve fibers extensively sprouted and thus markedly increased their density in the area where the accumulation of class II MHC antigen-expressing cells was evident.3. Substance P stimulated, but CGRP inhibited the proliferation of mitogenstimulated T lymphocytes when class II MHC antigen-expressing cells isolated from rat pulps were used as accessory cells.These findings suggest that sensory nerve fibers of the dental pulp influence upon the function of class II NHC antigen-expressing cells through release of neuropeptides from nerve endings. Such a neuro-immune interaction may play a part in the initiation of the immunodefense of the dental pulp.

本研究对牙髓的肽能神经纤维和第II类主要组织相容性复合体(MHC)抗原表达细胞进行了研究，并探讨了在生理和病理条件下这些结构的排列是否存在一定的空间关系。此外，还分析了神经肽对牙髓来源的II型MHC抗原表达细胞功能特性的影响。结果如下：1.免疫组织化学和激光共聚焦扫描显微镜显示，正常人和大鼠牙髓中表达MHCⅡ类抗原的细胞多分布于血管和神经纤维附近。在人牙本质龋齿和实验性龋病诱发的大鼠磨牙中，在与龋损相联系的牙本质小管的牙髓端附近的牙髓-牙本质边界上，观察到明显的II类MHC抗原表达细胞的局限性聚集。对人类龋齿的进一步分析表明，神经肽类神经纤维在MHCⅡ类抗原表达细胞聚集明显的区域广泛萌发，从而显著增加其密度。从大鼠牙髓分离的II类MHC抗原表达细胞作为辅助细胞时，P物质被刺激，而CGRP则抑制有丝分裂刺激的T淋巴细胞的增殖，提示牙髓感觉神经纤维通过神经末梢释放神经肽影响II类NHC抗原表达细胞的功能。这种神经免疫相互作用可能在启动牙髓的免疫防御中发挥作用。

项目成果

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Takashi Kochi（合著者）：“炎症和抗炎策略”Iyaku Journalsha，831（1997）

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Okiji T 等人：“实验诱导的大鼠磨牙根尖周炎症中神经和巨噬细胞之间的结构关系。”

Okiji T,et al.: "Phenotypic characteristics of class II molecule expressing cells in human dental pulp." Proceedings of the International Conference on Dentin/Pulp Complex 1995. 313-315 (1996)

Okiji T 等人：“人牙髓中 II 类分子表达细胞的表型特征。”

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Okiji,T.et al.: "Age-related charges in the immunoreactivity of the monocyte/macrophage system in rat molar pulp." Archires of Oral Biology. 41(5). 453-460 (1996)

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