Immune defense mechanisms of the dentin/pulp complex: Immunohistochemical analysis on the heterogeneity and kinetics of dendritic cells

牙本质/牙髓复合物的免疫防御机制：树突状细胞异质性和动力学的免疫组织化学分析

• 批准号: 17390508
• 负责人: OKIJI Takashi
• 金额: $ 9.18万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17390508/
• 关键词: dendritic cell; dentin; pulp complex; pulpitis; apical periodontitis; immunohistochemistry; ultrastructure; MHC class II molecule; co-stimulatory molecule; CD83; CD86; CD11c; 半導体レーザー

This study investigated dendritic cell (DC) subpopulations and co-stimulatory molecule-expressing cells in pulpitis and apical periodontitis by means of immunohistochemistry and transmission electron microscopy.(1) Pulpitis and apical periodontitis were induced in rat molars by making unsealed pulp exposures, and kinetics of DCs was investigated by means of immunoelectron microscopy for MHC class II molecules, CD11c, CD86 and OX62 (a marker for rat DC subpopulation). Results demonstrated that DCs in normal periodontal ligament and periapical lesions consisted of two subpopulations: the subpopulations differently expressed CD11c and OX62 and might differ in lineage, state of maturation and function. In the induced periapical lesions, CD86-expressing cells, comprising approximately 10% of MHC class II molecule-expressing cells, were frequently distributed in the vicinity of nerve fibers, suggesting the involvement of mature DC-nerve interaction in the development of periapical lesions. Moreover, kinetics of DCs during wound healing process of exposed rat molar pulps was investigated after 1VITA-capping, which constantly induced pulp healing with dentin bridge formation. It was demonstrated that DC-like cells showed an accumulation subjacent to the wound surface during initial healing process of the exposed pulps.(2) Immunohistochemistry and electron microscopy for human apical periodontitis revealed that DCs expressing BLA-DR were mainly distributed in lymphocyte-rich areas. By means of laser microdisection and RT-PCR analysis, it was demonstrated that upregulation of CD83 and CD86 on DCs and CD28 on T lymphocytes occurred in the lymphocyte-rich areas. These findings may indicate that DCs act as antigen presenting cells that stimulate T lymphocyte activation.

本研究采用免疫组织化学和透射电镜技术对牙髓炎和根尖牙周炎患者的树突状细胞亚群和共刺激分子表达细胞进行了研究。(1)采用非封闭牙髓暴露法诱导大鼠磨牙牙髓炎和根尖牙周炎，通过免疫电镜观察MHCⅱ类分子、CD11c、CD86和OX62（大鼠DC亚群标记物）对DC的影响。结果表明，正常牙周韧带和根尖周病变中的dc由两个亚群组成：这两个亚群表达CD11c和OX62不同，并且可能在谱系、成熟状态和功能上存在差异。在诱导的根尖周围病变中，cd86表达细胞（约占MHC II类分子表达细胞的10%）经常分布在神经纤维附近，提示成熟的dc -神经相互作用参与了根尖周围病变的发展。此外，我们还研究了暴露大鼠磨牙髓在1vita封盖后伤口愈合过程中树突状细胞的动力学，1vita封盖持续诱导牙本质桥形成的牙髓愈合。结果表明，在暴露髓体的初始愈合过程中，dc样细胞在创面附近聚集。(2)人根尖牙周炎的免疫组织化学和电镜显示，表达BLA-DR的树突状细胞主要分布在淋巴细胞富集区。通过激光显微切片和RT-PCR分析，我们发现dc上CD83和CD86的上调以及T淋巴细胞上CD28的上调发生在淋巴细胞富集区。这些发现可能表明dc作为抗原提呈细胞刺激T淋巴细胞活化。

项目成果

MTAに対するヒト歯髄反応に関する免疫組織化学的研究?直接覆髄後の細胞外基質の変化と細胞分化-

人牙髓对MTA反应的免疫组织化学研究？直接盖髓后细胞外基质和细胞分化的变化-

• 发表时间: 2007
• 作者: 吉羽永子,吉羽邦彦,重谷佳見;他
• 通讯作者: 他

Characteristics of resident dendritic cells in different regions of the rat periodontal ligament

大鼠牙周膜不同区域常驻树突状细胞的特征

• 发表时间: 2008
• 期刊: Cell Tissue Research 331
• 作者: Kaneko T;Kaneko T;Kaneko T
• 通讯作者: Kaneko T

Temporospatial expression of TIMPs during mouse incisor tooth development

小鼠门牙发育过程中TIMPs的时空表达

• 发表时间: 2006
• 作者: Okiji;T;et. al.;Yoshiba N;鞍立桃子;吉羽永子;Yoshiba N
• 通讯作者: Yoshiba N

「研究成果報告書概要(和文)」より

摘自《研究结果报告摘要（日文）》

• 发表时间: 2005
• 作者: Kawauchi;et. al.;Nishimura et al.;Dezawa et al.;Yoshizawa et al.;星野 幹雄;星野 幹雄
• 通讯作者: 星野 幹雄

Immunoelectron microscopic analysis of CD11c-positive dendritic cells in the periapical region of the rat periodontal ligament

大鼠牙周膜根尖周区CD11c阳性树突状细胞的免疫电镜分析

• 发表时间: 2006
• 作者: Okiji;T;et. al.;Yoshiba N;鞍立桃子;吉羽永子;Yoshiba N;Kaneko T
• 通讯作者: Kaneko T