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Effect of particle size on biocompatibility and biochemical evaluation of properties for various materials

粒径对各种材料生物相容性和生化性能评价的影响

基本信息

批准号：
14370623
负责人：
WATARI Fumio
金额：
$ 7.74万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

项目摘要

[Purpose]In dental material the powder/liquid method is abundantly used and abrasion powder arises in the sliding part of artificial joint. To study the effect of powders on health the size dependence of fine particles on biocompatibility was investigated by cell functional test and implantation test.[Method] Monodispersed particles were extracted by sedimentation for the size 0.5, 3, 10μm and by ultrafiltration for less than 300nm. The particles were thus prepared with the same size for different materials.[Size dependence] In cell functional test using the human neutrophil, periodontal ligament cell and rat macrophage, the cell survival rate was lowered and the emission of LDH, oxygen radical, cytokines TNF-α, IL-1β, IL-8, M-CSF increased as particle size became smaller, for Ti, Fe, Ni(each 500nm ; 3,10, 50, 150μm), TiO_2(30, 60, 300, 500nm ; 2μm), polylactic acid (1, 13μm). Especially, for the case of smaller than 10μm where phagocytosis was induced, the effect was remarkably pronou … More nced. The stimulatibility of these effects is lower by about 1/100〜1/1000 than the intracellular toxicity.[Material dependence]Both Ti whose ionic dissolution was negligible, confirmed by the ICP elemental analysis, and Fe, much more dissolvable, showed the similar size dependency. In Ni, cell survival rate was lower, emission of oxygen radical and cytokine was larger, the cell destruction was observed by microscopy, which was due to the stronger cytotoxicity of Ni.[Conclusion]In animal implantation test, Ti particles showed biocompatibility for the size larger than about 100μm, and inflammation for smaller than 50μm. For less than 10μm phagocytosis was induced and inflammation was continued for the long term. For the biocompatibility in the material with a macroscopic size the material-dependent, chemical effect through dissolved ions is generally dominant. For the range of μm〜nm, the material-independent, physical size effect origined from the relative size relation between particles and cell/tissue becomes tangible, even for the bioactive and bioinert materials. Less

[目的]在牙科材料中，粉液法应用较多，人工关节滑动部分产生磨损粉。为了研究粉末对健康的影响，通过细胞功能试验和植入试验研究了细颗粒对生物相容性的尺寸依赖性。[方法]采用沉降法提取粒径为0.5、3、10μm的单分散微粒，超滤法提取粒径小于300 nm的单分散微粒。因此，对于不同的材料，制备具有相同尺寸的颗粒。[Size在人中性粒细胞、牙周膜细胞和大鼠巨噬细胞的细胞功能试验中，随着粒径的减小，细胞存活率降低，LDH、氧自由基、细胞因子TNF-α、IL-1β、IL-8、M-CSF的释放增加，Ti、Fe、Ni TiO_2（30、60、300、500 nm; 2μm）;聚乳酸（1、13μm）。特别是对于直径小于10μm的噬菌体，诱导吞噬作用的效果更明显。 ...更多信息 nced。这些效应的刺激性比细胞内毒性低约1/100 ~ 1/1000。[材料依赖性]通过ICP元素分析确认的离子溶解可忽略的Ti和更易溶解的Fe都显示出类似的尺寸依赖性。镍的细胞存活率较低，氧自由基和细胞因子的释放量较大，显微镜下可见细胞破坏，这是由于镍的细胞毒性较强。[结论]在动物植入实验中，直径大于100μm的钛颗粒具有生物相容性，直径小于50μm的钛颗粒具有炎症反应。对于小于10μm，诱导吞噬作用，并长期持续炎症。对于具有宏观尺寸的材料的生物相容性，通过溶解离子的材料依赖性化学效应通常占主导地位。在μm ~ nm的范围内，源于颗粒与细胞/组织之间相对尺寸关系的与材料无关的物理尺寸效应变得切实可见，即使对于生物活性和生物惰性材料也是如此。少