Functions of Myc and c-Myc-binding proteins

Myc 和 c-Myc 结合蛋白的功能

基本信息

  • 批准号:
    14370736
  • 负责人:
  • 金额:
    $ 8.83万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    2002
  • 资助国家:
    日本
  • 起止时间:
    2002 至 2003
  • 项目状态:
    已结题

项目摘要

1).Identification of a novel transrepression pathway of c-Myc and its target geneWe have found that MM-1 bound to TIF13, a transcriptional corepressor, and that MM-1 recruited a corepressor complex, including HDAC1 and mSin3, to c-Myc, thereby leading to repressing c-Myc transcription activity. To identify target genes to this pathway, a MycER cell line harboring a dominant-negative form of TIFiβ, in which the corepressor complex was not recruited to c-Myc, was first established. DNA-microarray method was then applied to identify genes whose expressions were upregulated in this line compared to those in MycER cells. By this system we identified c-fms oncogene as the c-Myc-MM-1 repressed gene. It was then found that third E-box present m c-fins promoter was essential to be repressed by c-Myc.2). Identification of a novel degradation pathway of c-Myc.We have also identified Elongin B, 26Sproteasome subunits Rpt3 and Rpn12 as MM-1-associated proteins. Since these proteins functions for ub … More iquitination and degradation of proteins, we then tested a possibility that MM-1 plays a role in c-Myc degradation. The results indicate that MM-1 stimulated c-Myc degradation through the novel E3 ubiquitin ligase complex, including Spk2, Cullin 1, Elongon BThese findings indicate that MM-1 is a key protein that negatively regulates c-Myc function and that lost of these functions of MM-1 by mutations leads to tumor formation by c-Myc.3) AMY-1 was found to bind to the protein kinase A (P1(A) regulatory subunit type 2 (RII)-binding domains of several PKA-anchoring proteins, AKAPs, including AKAP149, S-AKAP84 and AKAP95, and to be localized in the cytoplasm or nuclei depending on associated AKAPs AMY-1 was also localized in the Golgi apparatus. AMY-1 bound to the first RII-binding domains of the brefeldin A-inhibited guanine nucleotide-exchange proteins BIG2 and BIG1 and colocalized with BIG2 even in the presence of brefeldin A, which inhibits guanine nucleotide-exchange activity of BIG2. AMY-1 was also found to be localized in the trans-Golgi apparatus. These results suggest that AMY-1 as a cofactor plays a role in guanine nucleotide-exchange reaction of BIG2 or BIG1. Less
1)识别C-MYC及其目标Genewe的新型变形途径已发现,MM-1与TIF13结合了转录核心压力器TIF13,并且MM-1募集了包括HDAC1和MSIN3的Corepressor络合物,包括HDAC1和MSIN3,包括C-MYC,从而导致反映C-MYC转录活性。为了识别该途径的靶基因,首先建立了含有主要阴性形式的TIFIβ的mycer细胞系,其中未募集Corepressor复合物为C-MYC。然后,将DNA微阵列方法应用于识别与脱粒细胞相比,其表达式更新的基因。通过该系统,我们将C-FMS癌基因确定为C-MYC-MM-1复制基因。然后发现第三个电子框呈现m c-fins启动子至关重要,必须由c-myc.2复制)。 C-Myc的新型降解途径的鉴定。我们还鉴定出Elongin B,26sproteasome unitiTs subunits RPT3和RPN12为MM-1相关蛋白。由于这些蛋白质在UB中的功能……更多的蛋白质降解和降解,因此我们测试了MM-1在C-MYC降解中起作用的可能性。结果表明,MM-1通过新型的E3泛素连接酶复合物刺激了C-MYC降解,包括SPK2,Cullin 1,Cullin 1,Elongon btheSe发现表明,MM-1是一种关键蛋白,负责调节C-Myc的功能,使MM-1通过突变构建tumor tumer tume tume tume tume tume tume tume tume tume tume tume tume tume tume tume tum a am amy-am a amcy.3) (P1(A) regulatory subunit type 2 (RII)-binding domains of several PKA-anchoring proteins, AKAPs, including AKAP149, S-AKAP84 and AKAP95, and to be localized in the cytoplasm or nuclei depending on associated AKAPs AMY-1 was also AMY-1 bound to the first RII-binding domains of the brefeldin A-inhibited guanine核苷酸交换蛋白BIG2和BIG1与BIG2共定位,即Brefeldin A抑制了鸟嘌呤的核苷酸 - 交换性AMY-1的活性,也发现了这些结果在Trans-golgi的结果中。较少的

项目成果

期刊论文数量(35)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Furusawa et al.: "Molecular cloning of the mouse AMY-1 gene and identification of the synergistic activation of the AMY-1 promoter by GATA-1 and Sp1"Genomics. 81. 221-233 (2003)
Furusawa 等人:“小鼠 AMY-1 基因的分子克隆以及 GATA-1 和 Sp1 对 AMY-1 启动子协同激活的鉴定”Genomics。
  • DOI:
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  • 期刊:
  • 影响因子:
    0
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  • 通讯作者:
Niki, et al.: "DJBP, a novel DJ-1-binding protein, negatively regulates the androgen receptor by recruiting histone deacetylase complex, and DJ-1 antagonizes this inhibition by abrogation of this complex."Mol.Cancer Res.. 1. 247-261 (2003)
Niki 等人:“DJBP 是一种新型 DJ-1 结合蛋白,通过募集组蛋白脱乙酰酶复合物对雄激素受体进行负调节,而 DJ-1 通过废除该复合物来拮抗这种抑制作用。”Mol.Cancer Res.. 1
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Taira, et al.: "DJ-1 plays a role in anti-oxidative stress to prevent cell death"EMBO Rep.. 5. 213-218 (2004)
Taira 等人:“DJ-1 在抗氧化应激中发挥作用,防止细胞死亡”EMBO Rep.. 5. 213-218 (2004)
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Yukitake et al.: "AAT-1, a novel testis-specific AMY-1-binding protein, forms a quarterly complex between AMY-1, A-kinase anchor protein 84 and a regulatory subunit of cAMP-dependent kinase, and is phosphorylated by its kinase."J.Biol.Chem.. 27. 45480-454
Yukitake 等人:“AAT-1 是一种新型睾丸特异性 AMY-1 结合蛋白,在 AMY-1、A-激酶锚蛋白 84 和 cAMP 依赖性激酶的调节亚基之间形成季度复合物,并被磷酸化
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  • 影响因子:
    0
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Okada et al.: "DJ-1, a target protein for an endocrine disrupter, participates in the fertilization in mice"Biol. Pharm. Bull.. 25. 853-856 (2002)
Okada 等人:“DJ-1 是一种内分泌干扰物的靶蛋白,参与小鼠的受精”Biol.
  • DOI:
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    0
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ARIGA Hiroyoshi其他文献

ARIGA Hiroyoshi的其他文献

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{{ truncateString('ARIGA Hiroyoshi', 18)}}的其他基金

Functional analysis of DJ-1, a causative gene for familial Parkinson's disease, and its pharmaceutical application
家族性帕金森病致病基因DJ-1的功能分析及其药物应用
  • 批准号:
    21390014
  • 财政年份:
    2009
  • 资助金额:
    $ 8.83万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Function of DJ-1, a causative gene for familial Parkinson's disease PARK7 and oncogene
家族性帕金森病致病基因 PARK7 和癌基因 DJ-1 的功能
  • 批准号:
    18390018
  • 财政年份:
    2006
  • 资助金额:
    $ 8.83万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Regulation of cell-cycle movement and cell transformation by c-Myc and its binding proteins
c-Myc 及其结合蛋白对细胞周期运动和细胞转化的调节
  • 批准号:
    12470490
  • 财政年份:
    2000
  • 资助金额:
    $ 8.83万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Regulation of cell-cycle movement and cell transformation by nuclear oncogenes.
核癌基因对细胞周期运动和细胞转化的调节。
  • 批准号:
    10470477
  • 财政年份:
    1998
  • 资助金额:
    $ 8.83万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Molecular mechanism of cell proliferation and appoptosis by the actions of transcription/replication factors in mammalian cells.
哺乳动物细胞中转录/复制因子作用的细胞增殖和凋亡的分子机制。
  • 批准号:
    08457599
  • 财政年份:
    1996
  • 资助金额:
    $ 8.83万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Establishment of gene therapy vector by using DNA replication origin and virus vector
利用DNA复制起点和病毒载体建立基因治疗载体
  • 批准号:
    07557147
  • 财政年份:
    1995
  • 资助金额:
    $ 8.83万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Conservative regulation of DNA replication and transcription in mammalian cells
哺乳动物细胞中 DNA 复制和转录的保守调控
  • 批准号:
    06454591
  • 财政年份:
    1994
  • 资助金额:
    $ 8.83万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Construction of expression vectors using initiation sites of DNA replication and their application to gene therapy
利用DNA复制起始位点构建表达载体及其在基因治疗中的应用
  • 批准号:
    04557105
  • 财政年份:
    1992
  • 资助金额:
    $ 8.83万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research (B)
Concerted mechanism of DNA replication initiation and transcription in mammalian cells
哺乳动物细胞中 DNA 复制起始和转录的协同机制
  • 批准号:
    03454489
  • 财政年份:
    1991
  • 资助金额:
    $ 8.83万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)

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Can diagnostic biomarkers for parkinsonian syndromes be measured in postmortem blood samples?
可以在死后血液样本中测量帕金森综合症的诊断生物标志物吗?
  • 批准号:
    10572535
  • 财政年份:
    2023
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用于 1 GHz 微线圈 NMR 光谱的台式无冷冻剂 23.5-T/25-mm-RT 孔径磁铁
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    10503243
  • 财政年份:
    2022
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A Benchtop Cryogen-Free 23.5-T/25-mm-RT-Bore Magnet for 1-GHz microcoil NMR Spectroscopy
用于 1 GHz 微线圈 NMR 光谱的台式无冷冻剂 23.5-T/25-mm-RT 孔径磁铁
  • 批准号:
    10708850
  • 财政年份:
    2022
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  • 项目类别:
Strategy to overcome the refractoriness of hematological malignancies through inhibition of intracellular signaling and immune enhancement
通过抑制细胞内信号传导和增强免疫来克服血液恶性肿瘤的难治性的策略
  • 批准号:
    20K08726
  • 财政年份:
    2020
  • 资助金额:
    $ 8.83万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
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