Evaluation of influences of air-borne particulates by molecular and cellular biological methods

通过分子和细胞生物学方法评估空气中颗粒物的影响

• 批准号: 14380257
• 负责人: YAGI Takashi
• 金额: $ 9.47万
• 依托单位: Osaka Prefecture University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14380257/
• 关键词: SPM; PM2.5; Air-borne particulates; Mutagenicity; Endocrine disruptors; Carcinogenicity; Aryl hydrocarbon; 芳香族炭化水素; リスク評価; 内分泌撹乱性

Air-borne particulates are major air pollutants in these decades. Especially, the particulate smaller than 2.5 μm diameter (PM2.5) is originated from diesel exhaust gas and considered to be a harmful substance to human health. Concentration of the PM2.5 in air has been shown positive correlation to induction of lung cancer. We obtained the air-borne particulates in Osaka, Higashi-osaka and Sakai Cities and analyzed their distribution of the particulate sizes and chemical composition. We also measured mutagenicity and endocrine disturbing ability of the particulates by bioassays and molecular biological methods.In Osaka and Higashi-osaka Cities (urban area), the distribution of the particulate size showed two peaks that were divided at approximately 2μm diameter. The mutagenicity and endocrine-disturbing activity were agree to the distribution of the small size peak (smaller than 2 μm diameter). In Sakai City (residential area), the endocrine-disturbing activity is stronger in the winter particulate sample than the summer one. The concentration of the particulate in air has gradually decreased year by year.As a result of molecular experiment, 3-nitrobenzanthrone(NBA), a strong mutagen contained in the particulate, caused G:C to T:A base change mutations in human and Escherichia coli cells. The NBA adducts in DNA was miscoded by the error-prone DNA polymerase V (umuDC), and they induced mutations.These results are valuable for prevention of lung cancer and for risk estimation of human population of the air-borne particulates.

近几十年来，空气中的颗粒物是主要的空气污染物。特别是直径小于2.5 μm的颗粒物（PM2.5）来源于柴油机尾气，被认为是对人体健康有害的物质。空气中PM2.5的浓度与肺癌的诱发呈正相关。我们获得了大坂，东大坂和酒井城市的空气中的颗粒物，并分析了它们的颗粒物的大小和化学成分的分布。在大坂市和东大坂市（市区），大气颗粒物的粒径分布呈现两个峰值，两个峰值的直径约为2μm。其致突变性和内分泌干扰活性与小峰（小于2 μm）的分布一致。在坂井市（居民区），内分泌干扰活动在冬季比夏季的颗粒物样品更强。大气颗粒物浓度逐年下降，分子生物学实验结果表明，大气颗粒物中含有的强诱变剂3-硝基苯蒽酮（NBA）可引起人和大肠杆菌细胞G：C → T：A碱基突变。DNA中的NBA加合物被易错DNA聚合酶V（umuDC）错误编码并诱发突变，这一结果对肺癌的预防和大气颗粒物对人群的风险评估有重要意义。

项目成果

大気浮遊粒子状物質の粒径分布と呼吸器沈着

空气中颗粒物的粒径分布和呼吸沉降

• 发表时间: 2002
• 期刊: 混相流 16(1)
• 作者: Guo.C.;森田昌行;溝畑朗
• 通讯作者: 溝畑朗

大気汚染物質3-ニトロベンズアントロン由来DNA付加体の損傷乗り越えDNA合成

DNA 合成克服了源自空气污染物 3-硝基苯并蒽酮的 DNA 加合物的损害

• 发表时间: 2004
• 期刊: 放射線生物研究 39・4
• 作者: 川藤優喜

Polycyclic aromatic hydrocarbons of diesel and gasoline exhaust and DNA aduct detection in calf thymus DNA and lymphocyte DNA of workers exposed to diesel exhaust

柴油、汽油尾气中多环芳烃及接触柴油尾气工人小牛胸腺DNA和淋巴细胞DNA中DNA加合物检测

• 发表时间: 2004
• 期刊: Polycyclic Aromatic Compounds 24
• 作者: Nakashima M;et al.;Kirsti Savela
• 通讯作者: Kirsti Savela

遺伝子組換え細胞,それを用いた多環芳香族化合物の分析方法および多環芳香族化合物の分析キット

转基因细胞、使用其的多环芳香族化合物的分析方法以及多环芳香族化合物的分析试剂盒

• 发表时间: 2003

Polycyclic aromatic hydrocarbons of diesel and gasoline exhaust and DNA adduct detection in calf thymus DNA and lymphocyte DNA of workers exposed to diesel exhaust.

柴油和汽油尾气中的多环芳烃以及接触柴油尾气的工人的小牛胸腺DNA和淋巴细胞DNA中的DNA加合物检测。

• 发表时间: 2004
• 期刊: Polycyclic Aromatic Compounds 24(4-5)
• 作者: Savela;K. et al.
• 通讯作者: K. et al.