Comparative genomic studies on the evolution of cyanobacteria and plastids

蓝藻和质体进化的比较基因组研究

• 批准号: 15370017
• 负责人: SATO Naoki
• 金额: $ 9.86万
• 依托单位: The University of Tokyo (2004)Saitama University (2003)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15370017/
• 关键词: Photosynthesis; Comparative genomics; Genome evolution; DNA polymerase; Visualization of nucleoids; プラスチド; シアノバクテリア; 光合成関連遺伝子; DNA結合タンパク質; 核様体

The aim of this study is to analyze the evolution of cyanobacterial endosymbiont to plastids using comparative genomic data based on prokaryotic and eukaryotic organisms. We identified some key components of the genomic machinery of plastids, and performed functional analysis of the components. Major results were the followings :1.We developed a new version (3.5) of the GCLUST software, which clusters predicted proteins of prokaryotic and eukaryotic organisms. This increased performance of clustering of large-scale genomic data.2.The 40 cyanobacterial genes that are included in the 37 clusters of functionally unknown genes, which are conserved in photosynthetic organisms, were disrupted. Fluorescence induction kinetic analysis indicated that many of them were affected in photosystems. Most of the Arabidopsis genes in the 37 gene clusters were light-induced, and the protein product of these genes were targeted to plastids. In addition, tag-lines that are disrupted in the genes in 20 of the clusters were analyzed. Three lines that showed visible phenotypes were found. There results demonstrate that the computationally extracted 37 gene clusters encode, in fact, plastid proteins of endosymbiont origin, which are novel factors involved in photosynthesis.3.Two DNA polymerases that are localized in the organelles of the unicellular red alga, Cyanidioschyzon merolae.4.Sulfite reductases in cyanobacteria, red alga, and green plants were analyzed with respect to DNA-binding activity and intracellular localization.5.A new technique was developed to visualize plastid nucleoids within live plant materials. Dynamism of plastid nucleoids in non-photosynthetic cells is now clearly demonstrated. During the division of plastid, nucleoids formed a network structure, which permitted even partition of nucleoids to daughter plastids.

本研究的目的是利用基于原核生物和真核生物的比较基因组数据分析蓝藻内共生体向质体的进化。我们确定了质体基因组机器的一些关键组件，并进行功能分析的组件。主要结果如下：1.我们开发了一个新版本的GCLUST软件（3.5），它可以对原核生物和真核生物的蛋白质进行聚类预测。这提高了大规模基因组数据聚类的性能。2.在光合生物中保守的37个功能未知基因簇中包含的40个蓝藻基因被破坏。荧光诱导动力学分析表明，它们中的许多在光系统中受到影响。在这37个基因簇中，大多数拟南芥基因是光诱导的，并且这些基因的蛋白质产物是靶向质体的。此外，分析了在20个簇中的基因中被破坏的标记线。结果表明，计算提取的37个基因簇实际上编码的是内共生体来源的质体蛋白，它们是参与光合作用的新因子。和绿色植物的DNA结合活性和细胞内定位进行了分析。5.发展了一种新的技术来显示活植物材料中的质体类核。非光合作用细胞中质体类核的动力学现在已被清楚地证明。在质体分裂过程中，类核形成了一个网状结构，使得类核可以均匀地分配到子质体中。

项目成果

Conserved temperature-dependent expression of RNA-binding proteins in cyanobacteria with different temperature optima

• DOI: 10.1016/s0378-1097(03)00503-2
• 发表时间: 2003-08-08
• 期刊: FEMS MICROBIOLOGY LETTERS
• 影响因子: 2.1
• 作者: Ehira, S;Hamano, T;Sato, N
• 通讯作者: Sato, N

T.Nomata, Y.Kabeya, N.Sato: "Cloning and characterization of glycine-rich RNA-binding protein cDNAs in the moss Physcomitrella patens."Plant Cell Physiol.. 44. 48-56 (2004)

T.Nomata、Y.Kabeya、N.Sato：“小立碗藓中富含甘氨酸的 RNA 结合蛋白 cDNA 的克隆和表征。”植物细胞生理学.. 44. 48-56 (2004)

Genome sequence of the ultrasmall unicellular red alga Cyanidioschyzon merolae 10D

• DOI: 10.1038/nature02398
• 发表时间: 2004-04-08
• 期刊: NATURE
• 影响因子: 64.8
• 作者: Matsuzaki, M;Misumi, O;Kuroiwa, T
• 通讯作者: Kuroiwa, T

Use of segment-based microarray in the analysis of global gene expression in response to various environmental stresses in the cyanobacterium Anabaena sp PCC 7120

• DOI: 10.2323/jgam.50.1
• 发表时间: 2004-02-01
• 期刊: JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
• 影响因子: 1.2
• 作者: Sato, N;Ohmori, M;Kuhara, S
• 通讯作者: Kuhara, S

Organization, developmental dynamics and evolution of the plastid nucleoid.

质体核的组织、发育动力学和进化。

• 发表时间: 2003
• 期刊: Int.Rev.Cytol. 232
• 作者: N.Sato;K.Terasawa;K.Miyajima;Y.Kabeya
• 通讯作者: Y.Kabeya