Structure and function of plastid nucleoid

质体核的结构和功能

• 批准号: 11440232
• 负责人: SATO Naoki
• 金额: $ 10.11万
• 依托单位: SAITAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11440232/
• 关键词: Plastid nucleoid; RNA polymerase; in vitro transcription; DNA-binding protein; sulfite reductase; HUタンパク質; ヘパリン; 真核・原核ハイブリッド

Plastid nucleoid, which is a functional unit of plastid DNA, is considered as a complex or hybrid of prokaryotic DNA with eukaryotic DNA-binding proteins. Based on this standpoint, we tried to identify the components of plastid nucleoids and to analyze their function in the replication and transcription of plastid DNA. The publication of the complete nucleotide sequence of the genome of Arabidopsis thaliana made us conclude that many of the prokaryotic nucleoid proteins are not present in higher plants. Examples are the HU protein and Dps protein that are ubiquitously present in prokaryotes but these are not present in the chloroplast of A. thaliana.Many of the nucleoid proteins must have been acquired during the evolution of land plants by duplication or modification of the nuclear genes encoding eukaryotic DNA-binding proteins. These results prompted us to put forward a hypothesis on the discontinuous evolution of plastid genomic machinery.

类叶绿体是原核生物DNA与真核DNA结合蛋白的复合体或杂交体，是叶绿体DNA的功能单位。基于这一观点，我们试图鉴定叶绿体类核的成分，并分析它们在叶绿体DNA复制和转录中的功能。拟南芥基因组全序列的公布使我们得出结论：许多原核类核蛋白在高等植物中并不存在。例如HU蛋白和DPS蛋白，它们普遍存在于原核生物中，但在拟南芥的叶绿体中不存在。许多类核蛋白肯定是在陆地植物进化过程中通过复制或修饰编码真核DNA结合蛋白的核基因而获得的。这些结果促使我们提出了关于叶绿体基因组机制不连续进化的假说。

项目成果

Hashimoto,K.and Sato,N.: "Characterization of the mitochondrial nad7 gene in Physcomitrella patens : Similarity with angiosperm nad7 genes."Plant Science. (印刷中). (2001)

Hashimoto, K. 和 Sato, N.：“小立碗藓中线粒体 nad7 基因的特征：与被子植物 nad7 基因的相似性”（出版中）。

N.Sato., N.Rolland., M.A.Block, J.Joyard: "Do plastid envelope membranes play a role in the expression of the plastid genome?"Biochimie. 81. 619-629 (1999)

N.Sato.、N.Rolland.、M.A.Block、J.Joyard：“质体包膜在质体基因组的表达中发挥作用吗？”Biochimie。

N. Sato, N. Rolland, M. A. Block, J. Joyard: "Do plastid envelope membranes play a role in the expression of the plastid genome?"Biochimie. 81. 619-629 (1999)

N. Sato、N. Rolland、M. A. Block、J. Joyyard：“质体包膜在质体基因组的表达中发挥作用吗？”Biochimie。

N.Sato, N.Ohta: "DNA-binding specificity and dimerization of the DNA-binding domain of the PEND protein in the chloroplast envelope membrane"Nucleic Acids. Res.. 29. 2244-2250 (2002)

N.Sato，N.Ohta：“叶绿体包膜中 PEND 蛋白 DNA 结合域的 DNA 结合特异性和二聚化”核酸。

得平茂樹,佐藤直樹: "窒素固定能をもつAnabaena属のラン藻の遺伝子操作"化学と生物. 39. 121-126 (2001)

Shigeki Tokuhira、Naoki Sato：“具有固氮能力的鱼腥藻属蓝绿藻的基因操纵”，化学与生物学，39. 121-126 (2001)。