Analysis of molecular mechanisms of inner ear development by characterizing a secreted molecule, OC29, isolated from rat otocyst.

通过表征从大鼠耳囊中分离的分泌分子 OC29 来分析内耳发育的分子机制。

• 批准号: 15390105
• 负责人: TASHIRO Kei
• 金额: $ 9.79万
• 依托单位: Kyoto Prefectural University of Medicine (2004)Kyoto University (2003)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15390105/
• 关键词: stem cell; inner ear; secreted molucule; signal-sequence trap; otocyst; soluble factor; rat; development; 内耳幹細泡; 分泌タンンパク質; RO109

The mammalian inner ear consists of six distinct sensory organs : three cristae of the semicircular canals, two maculae of the saccule and utricle, and the organ of Corti of the cochlea. Despite its complexity, the inner ear derives from a simple cyctic structure known as an otocyst. However, molecular mechanisms underlying inner ear development are largely unknown. To identify molecule(s) involved in the formation and/or further differentiation of the otocyst, we isolated a secreted molecule, OC29, from a rat otocyst cDNA library by the signal sequence trap method. OC29 was revealed to be a rat homologue of human WFIKKN. OC29 is preferentially expressed in the developing inner ear and dorsal neural tube. In the inner ear, the expression of OC29 is first detectable at embryonic day 11.5 (E11.5), broadly in the dorsolateral region of the otocyst, which gives rise to the vestibular organ. At E12.5, the expression of OC29 becomes restricted to the presumptive sensory organ, mainly to the BMP4-positive presumptive cristae, and expression becomes reduced at later stages. These results suggested that OC29 may have a role in the early development of the inner ear sensory organ, particularly in the formation of the cristae of the semicircular canals.

哺乳动物的内耳由六个不同的感觉器官组成：三个半规管嵴，两个囊状和耳室斑，以及耳蜗的Corti器官。尽管内耳很复杂，但它起源于一个简单的循环结构，即耳囊肿。然而，内耳发育的分子机制在很大程度上是未知的。为了确定参与耳囊肿形成和/或进一步分化的分子，我们用信号序列陷阱法从大鼠耳囊肿cDNA文库中分离出一个分泌分子OC29。OC29被发现是人类WFIKKN的大鼠同源物。OC29在发育中的内耳和背神经管中优先表达。在内耳中，OC29的表达在胚胎第11.5天（E11.5）首次被检测到，广泛存在于前庭器官形成的耳囊肿背外侧区域。在E12.5时，OC29的表达仅限于推定感觉器官，主要是bmp4阳性的推定嵴，并且在后期表达减少。这些结果表明，OC29可能在内耳感觉器官的早期发育中起作用，特别是在半规管嵴的形成中。

项目成果

Molecular cloning of human dectin-2.

• DOI: 10.1111/j.0022-202x.2004.22602.x
• 发表时间: 2004-06
• 期刊: The Journal of investigative dermatology
• 作者: N. Kanazawa;K. Tashiro;K. Inaba;M. Lutz;Y. Miyachi

Expression of Btc12, a novel member of Btc1 family, during development of the central nervous system.

Btc12（Btc1 家族的新成员）在中枢神经系统发育过程中的表达。

• 发表时间: 2004
• 期刊: Brain Res.Dev.Brain Res. 153
• 作者: Michishita;M. et al.
• 通讯作者: M. et al.

Expression of Btcl2,a novel member of Btcl family, during development of the central nervous system.

Btcl2（Btcl家族的新成员）在中枢神经系统发育过程中的表达。

• 发表时间: 2004
• 期刊: Brain Res.Dev. Brain Res. 153・1
• 作者: Michishita;M. et al.
• 通讯作者: M. et al.

Estrogen promotes differentiation and survival of dopaminergic neurons derived from human neural stem cells

• DOI: 10.1002/jnr.20362
• 发表时间: 2005-02-01
• 期刊: JOURNAL OF NEUROSCIENCE RESEARCH
• 影响因子: 4.2
• 作者: Kishi, Y;Takahashi, J;Hashimoto, N
• 通讯作者: Hashimoto, N

Signaling and immune regulatory role of the dendritic cell immunoreceptor (DCIR) family lectins: DCIR, DCAR, dectin-2 and BDCA-2.

• DOI: 10.1016/j.imbio.2004.03.004
• 发表时间: 2004-08
• 期刊: Immunobiology
• 影响因子: 2.8
• 作者: N. Kanazawa;K. Tashiro;Y. Miyachi