Systematization of Non-invasive Evaluation of Atherosclerosis in Clinical Laboratory Medicine

临床检验医学中动脉粥样硬化无创评估的系统化

• 批准号: 15390177
• 负责人: OSHIMA Tetsuya
• 金额: $ 7.74万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15390177/
• 关键词: atherosclerosis; endothelium; reactive hyperemia; platelets; shear stress; hypertension; artery; inflammation

1.The purpose of the present study was to systematize the non-invasive evaluation of atherosclerosis in clinical laboratory medicine. We establishied the normal ranges of several evaluations and compared these values amang several atherosclerotic diseases.2.We determined high-sensitive C reactive protein(CRP) and IgG antibodies to Helicobacter pylori, Chlamydia pneumoniae and cytomegalovirus. We measured endothelium-dependent and-independent vasodilations in brachial artery and the intima-media thickness in common carotid artery by high frequency ultrasonographic imagings. Pulse wave velocity was obtained by automatic device. Measurements were performed in healthy subjects (normal group), patients with hypertension, diabetes or hyperlipidemia (mild atherosclerotic group) and patients with overt cardiovascular diseases (severe atherosclerotic group).3.Flow-mediated vasodilation was decreased in patients with mild or severe atherosclerosis. Endothelium-independent vasodilation was similar in the three groups and negatively correlated with age. Pulse wave velocity and carotid intima-media thickness were increased in patients with mild-and severe atherosclerosis. Thus, atherosclerosis risk factors are associated with endothelial dysfunction and thickness and stiffuess of arteries.4.CRP was increased in patients with severe atherosclerosis in comparison with healthy subjects. IgG antibodies to three pathogens were similar in the three groups. CRP was increased and flow-mediated vasodilation was athenuated in subjects seropositive to Helicobacter pylori.5.Index of endothelial function, vascular function, platelet function and serum CRP have been reproducible in our study period.6.In conclusion, atherosclerosis can be evaluated by non-invasive evaluations of vascular function and structure and systemic inflammatory responses.

1.本研究的目的是系统化的非侵入性评价动脉粥样硬化的临床实验室医学。我们建立了几种评价指标的正常范围，并比较了这些指标在几种动脉粥样硬化疾病中的价值。2.检测了高敏C反应蛋白（CRP）和幽门螺杆菌、肺炎衣原体和巨细胞病毒的IgG抗体。应用高频超声检测肱动脉内皮依赖性和非内皮依赖性舒张功能及颈总动脉内膜中层厚度。脉搏波传导速度由自动装置测定。分别对健康人（正常组）、高血压、糖尿病、高脂血症患者（轻度动脉粥样硬化组）和明显心血管疾病患者（重度动脉粥样硬化组）进行检测。内皮非依赖性血管舒张在三组中相似，并与年龄呈负相关。轻、重度动脉粥样硬化患者脉搏波传导速度和颈动脉内膜中层厚度均增加。因此，动脉粥样硬化的危险因素与内皮功能障碍、动脉的厚度和僵硬度有关。4.与健康人相比，严重动脉粥样硬化患者的CRP升高。三组中三种病原体的IgG抗体相似。结论：动脉粥样硬化可以通过无创性的血管功能和结构以及全身炎症反应来评估。

项目成果

Effect of amlodipine and cilazapril treatment on platelet Ca^<2+> handing in spontaneously hypertensive rats

氨氯地平和西拉普利治疗对自发性高血压大鼠血小板Ca^2处理的影响

• 发表时间: 2003
• 期刊: Hypertension Research 26
• 作者: Yamasaki;S.;Tetsuya Oshima
• 通讯作者: Tetsuya Oshima

Tetsuya Oshima: "Effect of amlodipine and cilazapril treatment on platelet Ca^<2+> handing in spontaneously hypertensive rats"Hypertension Research. 26. 901-906 (2003)

Tetsuya Oshima：“氨氯地平和西拉普利治疗对自发性高血压大鼠血小板Ca^2>的影响”高血压研究。

Cardiac angiotensin II type 2 receptor activates the kinin/NO system and inhibits fibrosis

• DOI: 10.1161/01.hyp.0000050101.90932.14
• 发表时间: 2003-01-01
• 期刊: HYPERTENSION
• 影响因子: 8.3
• 作者: Kurisu, S;Ozono, R;Matsubara, H
• 通讯作者: Matsubara, H

Relation between C reactive protein concentrations and coronary microvascular endothelial function

• DOI: 10.1136/hrt.2003.022269
• 发表时间: 2004-06
• 期刊: Heart
• 影响因子: 5.7
• 作者: H. Teragawa;Y. Fukuda;K. Matsuda;Keiko Ueda;Y. Higashi;Tetsuya Oshima;Masao Yoshizumi;K. Chayama

Smoking activates Rho-kinase in smooth muscle cells of forearm vasculature in humans

• DOI: 10.1161/01.hyp.0000067062.92836.9e
• 发表时间: 2003-05-01
• 期刊: HYPERTENSION
• 影响因子: 8.3
• 作者: Noma, K;Higashi, Y;Chayama, K
• 通讯作者: Chayama, K